Periodontal pathogens in subgingival plaque of HIV-positive subjects with chronic periodontitis

Abstract Background Initial periodontal therapy (IPT) is cornerstone of periodontal therapy and the first step to control of periodontal risk factors. Scaling and root planing are used to treat root surface irregularities and remove virulent factors caused by periodontal pathogens. This procedure also incorporated into periodontal surgery. To elucidate the effects of IPT on prevalence of Epstein-Barr virus (EBV) DNA and Porphyromonas gingivalis, we used subgingival plaque samples from chronic periodontitis (CP) patients. Methods Seventeen CP patients were recruited and measured periodontal clinical parameters such as probing pocket depth (PD) and bleeding on probing (BOP), and subgingival plaque samples were collected from two periodontal sites with PD of <3 mm (healthy sites: HS) or >5 mm (periodontitis sites: PS) at first visit and after IPT. Plaque samples were subjected to a real-time PCR to detect EBV DNA and P. gingivalis. Results EBV DNA and P. gingivalis were detected 9 (52.9%) and 14 (82.3%) in the subgingival samples from HS, and 13 (76.5%) and 14 (82.3%) in the PS at first visit. After IPT, number of detections of EBV DNA and P. gingivalis were decreased to 5 (29.4%) and 13 (76.5%) in the HS, and 9 (52.9%) and 10 (58.8%) in the PS. Significant improvements in PD and BOP were observed after IPT in PS. Coexistence of EBV DNA and P. gingivalis in the subgingival samples from PS at first visit (12; 70.6%) were significantly decreased after IPT (6; 35.3%). Conclusion These results suggest that the IPT was effective in improvement of clinical parameters such as PD and BOP and reducing the coexistence of EBV and P. gingivalis in the subgingival plaque from PS. However, IPT could not eradicate the EBV and P. gingivalis. Further research would be necessary for improving the periodontal treatment strategy.

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Effects of initial periodontal therapy on the prevalence of Epstein-Barr virus DNA and Porphyromonas gingivalis in Japanese chronic periodontitis patients

10.21203/rs.2.16315/v1 ◽

2019 ◽

Author(s):

Ayako Kato ◽

Yorinobu Ikeda ◽

Kenichi Imai ◽

Yorimasa Ogata

Keyword(s):

Porphyromonas Gingivalis ◽

Chronic Periodontitis ◽

Epstein Barr Virus ◽

Periodontal Therapy ◽

Subgingival Plaque ◽

Clinical Parameters ◽

Periodontal Pathogens ◽

Barr Virus ◽

Ebv Dna ◽

Epstein Barr

Abstract Background Initial periodontal therapy (IPT) is cornerstone of periodontal therapy and the first step to control of periodontal risk factors. Scaling and root planing are used to treat root surface irregularities and remove virulent factors caused by periodontal pathogens. This procedure also incorporated into periodontal surgery. To elucidate the effects of IPT on prevalence of Epstein-Barr virus (EBV) DNA and Porphyromonas gingivalis , we used subgingival plaque samples from chronic periodontitis (CP) patients.Methods Seventeen CP patients were recruited and determined measured periodontal status clinical parameters such as by probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP) and an X-ray examination, and subgingival plaque samples were collected from two periodontal sites with PD of < 3 mm (healthy sites: HS) or > 5 mm (periodontitis sites: PS) at first visit and after IPT. Plaque samples were subjected to a real-time PCR to detect EBV DNA and P. gingivalis.Results EBV DNA and P. gingivalis were detected 9 (52.9%) and 14 (82.3%) sites within the subgingival samples from HS, and 13 (76.5%) and 14 (82.3%) sites within the PS at first visit. After IPT, number of detections of EBV DNA and P. gingivalis were decreased to 5 (29.4%) and 13 (76.5%) sites within the subgingival samples from HS, and 9 (52.9%) and 10 (58.8%) sites within the PS. Significant improvements in PD and BOP were observed after IPT in PS. Coexistence of EBV DNA and P. gingivalis in the subgingival samples from PS at first visit (12 sites; 70.6%) were significantly decreased after IPT (6 sites; 35.3%).Conclusion These results suggest that the IPT was effective in improvement of clinical parameters such as PD and BOP and reducing the coexistence of EBV and P. gingivalis in the subgingival plaque from PS. However, IPT could not eradicate the EBV and P. gingivalis . Further research would be necessary for improving the periodontal treatment strategy.

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Detection of Herpesviruses and Periodontal Pathogens in Subgingival Plaque of Patients With Chronic Periodontitis, Generalized Aggressive Periodontitis, or Gingivitis

Journal of Periodontology ◽

10.1902/jop.2008.070388 ◽

2008 ◽

Vol 79 (12) ◽

pp. 2313-2321 ◽

Cited By ~ 50

Author(s):

Ana Vitória Imbronito ◽

Osmar Shizuo Okuda ◽

Nivea Maria de Freitas ◽

Roberto Fraga Moreira Lotufo ◽

Fabio Daumas Nunes

Keyword(s):

Chronic Periodontitis ◽

Aggressive Periodontitis ◽

Subgingival Plaque ◽

Periodontal Pathogens

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Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens

Revista de Chimie ◽

10.37358/rc.17.12.5993 ◽

2018 ◽

Vol 68 (12) ◽

pp. 2853-2856 ◽

Cited By ~ 3

Author(s):

Igor Jelihovschi ◽

Cristian Drochioi ◽

Aida Corina Badescu ◽

Raoul Vasile Lupusoru ◽

Alexandra Elena Munteanu ◽

...

Keyword(s):

Periodontal Disease ◽

Periodontal Diseases ◽

Pcr Analysis ◽

Treponema Denticola ◽

Subgingival Plaque ◽

Periodontal Pathogens ◽

Radiographic Evidence ◽

Gingival Bleeding ◽

As Species ◽

Qpcr Quantification

The diagnosis of periodontal disease is mainly based on use of clinical and radiographic evidence. In this study we employed a quantitative PCR analysis of Aggregatibacter actinomycetemcomitans and Treponema denticola as species strongly involved in periodontal diseases, burden in periodontal pockets to detect the main sampling factors that interfere with qPCR results. From 22 patients with advanced periodontal disease, subgingival plaque was comparatively collected by paper points and periodontal Gracey curettes. Samples were collected from the same situs in presence of gingival bleeding and absence of bleeding. The concordance and agreement of results between samples were assessed. The present study demonstrates that subgingival plaque sampling with sterile absorbable paper points is often accompanied by gingival bleeding resulting in quantification biases of periodontal pathogens.

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In Situ Anabolic Activity of Periodontal Pathogens Porphyromonas gingivalis and Filifactor alocis in Chronic Periodontitis

Scientific Reports ◽

10.1038/srep33638 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 14

Author(s):

Ralee Spooner ◽

Kris M. Weigel ◽

Peter L. Harrison ◽

KyuLim Lee ◽

Gerard A. Cangelosi ◽

...

Keyword(s):

Porphyromonas Gingivalis ◽

Chronic Periodontitis ◽

Periodontal Pathogens ◽

Anabolic Activity ◽

Filifactor Alocis

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Evaluation of Effect of Nd:YAG and Er:YAG Lasers on the Periodontal Pathogens: A Systematic Review

10.37191/mapsci-jdr-1(1)-003 ◽

2020 ◽

Author(s):

Hosein Eslami

Keyword(s):

Systematic Review ◽

Chronic Periodontitis ◽

Complementary Therapy ◽

Root Surface ◽

Laser Wavelength ◽

Time Interval ◽

Periodontal Pathogens ◽

Probing Depth ◽

Treatment Procedures ◽

The Given

Introduction: By removing the periodontal pathogens and even the invasive bacteria attacking the tissues as well as the lack of normal periodontal treatment procedures, using a laser, as a complementary therapy, can have positive and useful impacts. The present study aims at investigating the impacts of Nd:YAG and Er:YAG laser on periodontal pathogens. Materials and Methods: In this systematic review, articles in the databases of Google Scholar, PubMed and Scopus were searched within 2000-2020. All information is classified into a table. Results: In searching the aforementioned databases and considering the given time interval, 78 articles were found in connection to the searched keywords and abstracts. Once the repeated titles and articles were deleted, 25 studies were selected which were decreased to 16 by the meticulous review by the assessment team. The full text of these 16 articles was reviewed. To make the final choice, 8 articles had the inclusion criteria which included the objectives of this study. Conclusion: To use the laser in treating the chronic periodontitis, an evidence-based study seems to be conducted. A few scientific pieces of evidence are indicating the advantage of a special laser wavelength in the common treatments.Based on most researches reviewed in this study, the results of the complementary application of Nd:YAG and Er:YAG lasers in treating the chronic periodontitis are the better than the ones obtained from the normal teeth scaling and planning the teeth root surface in terms of the reduced probing depth and subgingival bacteria population. The decision was made to use the laser in periodontal surgery based on the advantages such as hemostasis and less pain after the treatment. However, more studies are needed to apply the laser in periodontal treatments.

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Bullet for periodontal disease in future: Chemically modified tetracyclines

Journal of Cellular Biotechnology ◽

10.3233/jcb-210040 ◽

2021 ◽

pp. 1-7

Author(s):

Shivani Sachdeva ◽

Ameet Mani ◽

Harish Saluja

Keyword(s):

Chronic Periodontitis ◽

Periodontal Ligament ◽

Host Response ◽

Alveolar Bone ◽

Gastrointestinal Toxicity ◽

Collagenolytic Activity ◽

Periodontal Pathogens ◽

Oral Biofilm ◽

Chemically Modified ◽

Chemically Modified Tetracyclines

Chronic periodontitis is nowadays popularly regarded as Dysbiosis, [1] which causes destruction of tissues rich in collagen like periodontal ligament, alveolar bone and gingival connective tissue. The oral biofilm comprises many periodontal pathogens better regarded as ‘triggers’ in causing chronic periodontitis. Since, not everyone will be affected in the same manner due to periodontal pathogens. Some might not elicit a host response while, the others might have exaggerated response. So, host modulation therapy came into existence to counteract the exaggerated host response. The chemically modified tetracyclines (CMTs) have emerged to inhibit the inflammatory response or to reduce the collagenolytic activity of host. Though a derivative of tetracyclines, it still lacks an antimicrobial action and hence, can be used for periodontitis for longer duration with no adverse effects of gastrointestinal toxicity which parent tetracyclines have.

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