Bullet for periodontal disease in future: Chemically modified tetracyclines

2021 ◽  
pp. 1-7
Author(s):  
Shivani Sachdeva ◽  
Ameet Mani ◽  
Harish Saluja
Keyword(s):  
Chronic Periodontitis ◽  
Periodontal Ligament ◽  
Host Response ◽  
Alveolar Bone ◽  
Gastrointestinal Toxicity ◽  
Collagenolytic Activity ◽  
Periodontal Pathogens ◽  
Oral Biofilm ◽  
Chemically Modified ◽  
Chemically Modified Tetracyclines

Chronic periodontitis is nowadays popularly regarded as Dysbiosis, [1] which causes destruction of tissues rich in collagen like periodontal ligament, alveolar bone and gingival connective tissue. The oral biofilm comprises many periodontal pathogens better regarded as ‘triggers’ in causing chronic periodontitis. Since, not everyone will be affected in the same manner due to periodontal pathogens. Some might not elicit a host response while, the others might have exaggerated response. So, host modulation therapy came into existence to counteract the exaggerated host response. The chemically modified tetracyclines (CMTs) have emerged to inhibit the inflammatory response or to reduce the collagenolytic activity of host. Though a derivative of tetracyclines, it still lacks an antimicrobial action and hence, can be used for periodontitis for longer duration with no adverse effects of gastrointestinal toxicity which parent tetracyclines have.

scholarly journals Role of sea buckthorn oil in management of chronic periodontitis: follow-up study

2016 ◽  
Vol 4 (2) ◽  
pp. 33 ◽  
Author(s):  
Anamika Sharma ◽  
Shatakshi Sharma ◽  
Shivi Khattri ◽  
Harshita Garg
Keyword(s):  
Chronic Periodontitis ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Sea Buckthorn ◽  
Long Term Results ◽  
Treatment Modalities ◽  
Adjunctive Treatment ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Sea Buckthorn Oil

Periodontitis is an inflammatory condition resulting in loss of periodontal ligament and alveolar bone. Nonsurgical therapy remains the cornerstone of periodontal treatment. For sustained plaque control it plays an important role in achieving successful long-term results for the care of Chronic Periodontitis subjects. Proper maintenance and patient hygiene plays a vital role in suppression of re-colonization of micro-organism. Many adjunctive treatment modalities along with mechanical debridement have been clinically used and investigated for their efficacy. Systemic antioxidants in conjunction with scaling and root planing can offer additional effects and can be used as an adjunctive treatment. Patients with periodontal disease display increased PMN number and activity, resulting in high degree of free radical release culminating in heightened oxidative damage to gingival tissues, periodontal ligament and alveolar bone. Damage mediated by free radicals can be mitigated by "antioxidant defense system ".Sea buckthorn oil is an omega-7 fatty acid antioxidant which has unique botanical and nutritional properties thereby benefiting mucous membrane by reducing inflammation, improving wound healing and showing excellent antibacterial property. In this research patients were prescribed systemic antioxidant after the phase 1 therapy ie scaling and root planing to observe its effects on the various clinical parameters.

scholarly journals TheTreponema denticolaChymotrypsin-Like Protease Dentilisin Induces Matrix Metalloproteinase-2-Dependent Fibronectin Fragmentation in Periodontal Ligament Cells

2010 ◽  
Vol 79 (2) ◽  
pp. 806-811 ◽  
Author(s):  
Di Miao ◽  
J. Christopher Fenno ◽  
John C. Timm ◽  
Nam Eok Joo ◽  
Yvonne L. Kapila
Keyword(s):  
Periodontal Disease ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Inflammatory Responses ◽  
Tissue Homeostasis ◽  
Matrix Metalloproteinase 2 ◽  
Periodontal Ligament Cells ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Culture Supernatants

ABSTRACTPeriodontal disease is a bacterially mediated chronic inflammatory disease that results in destruction of the periodontal ligament (PDL) and alveolar bone that surround and support the dentition. While their precise roles are not well understood, periodontal pathogens, includingTreponema denticola, are believed to initiate the destructive inflammatory responses and dysregulation of tissue homeostasis that characterize the disease. These responses are believed to result from both proinflammatory effects of acylated bacterial membrane components (lipopolysaccharides and lipoproteins) and degradative effects of secreted bacterial proteases. Host-derived matrix metalloproteinases (MMPs) are key enzymes both in tissue homeostasis and tissue destruction. MMP expression is modulated in part by specific proteolytic fragments of fibronectin (FN), which are associated with periodontal disease. FN is a predominant extracellular matrix component in the periodontium. We examined the ability ofTreponema denticolaand its acylated outer membrane PrtP protease complex to induce both activation of MMP-2 and generation of FN fragments in human PDL cell culture supernatants.T. denticolaparent and isogenic mutant strains, as well as MMP-2 small interfering RNA and specific inhibitors of MMP-2 and PrtP activity, were used to examine protein expression, gelatinolytic activity, and FN fragmentation in culture supernatants.T. denticolaand its purified protease induced both MMP-2 activation and FN fragmentation. Here, we demonstrate that PrtP proteolytic activity induces the activation of MMP-2 and that active MMP-2 is required for FN fragmentation. These results suggest a specific mechanism by which theT. denticolaprotease may disrupt homeostatic processes required for the maintenance of periodontal health.

scholarly journals In Situ Anabolic Activity of Periodontal Pathogens Porphyromonas gingivalis and Filifactor alocis in Chronic Periodontitis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ralee Spooner ◽  
Kris M. Weigel ◽  
Peter L. Harrison ◽  
KyuLim Lee ◽  
Gerard A. Cangelosi ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Chronic Periodontitis ◽  
Periodontal Pathogens ◽  
Anabolic Activity ◽  
Filifactor Alocis

scholarly journals Oral biofilm challenge regulates the RANKL-OPG system in periodontal ligament and dental pulp cells

2011 ◽  
Vol 50 (1) ◽  
pp. 6-11 ◽  
Author(s):  
Georgios N. Belibasakis ◽  
Andre Meier ◽  
Bernhard Guggenheim ◽  
Nagihan Bostanci
Keyword(s):  
Dental Pulp ◽  
Periodontal Ligament ◽  
Dental Pulp Cells ◽  
Oral Biofilm ◽  
Pulp Cells

scholarly journals Osteoclastic effects of mBMMSCs under compressive pressure during orthodontic tooth movement

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jing Wang ◽  
Delong Jiao ◽  
Xiaofeng Huang ◽  
Yuxing Bai
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Normal Saline ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Mouse Bone Marrow ◽  
Micro Ct ◽  
Tail Vein

Abstract Background During orthodontic tooth movement (OTM), alveolar bone remodelling is closely related to mechanical force. It is unclear whether stem cells can affect osteoclastogenesis to promote OTM. This study aimed to investigate the role of mouse bone marrow mesenchymal stem cells (mBMMSCs) under compression load in OTM. Methods A mouse OTM model was established, and GFP-labelled mBMMSCs and normal saline were injected into different groups of mice by tail vein injection. OTM distance was measured using tissue specimens and micro-computed tomography (micro-CT). The locations of mBMMSCs were traced using GFP immunohistochemistry. Haematoxylin-eosin staining, tartrate-resistant acid phosphate (TRAP) staining and immunohistochemistry of Runx2 and lipoprotein lipase were used to assess changes in the periodontal ligament during OTM. mBMMSCs under compression were co-cultured with mouse bone marrow-derived macrophages (mBMMs), and the gene expression levels of Rankl, Mmp-9, TRAP, Ctsk, Alp, Runx2, Ocn and Osterix were determined by RT-PCR. Results Ten days after mBMMSCs were injected into the tail vein of mice, the OTM distance increased from 176 (normal saline) to 298.4 μm, as determined by tissue specimen observation, and 174.2 to 302.6 μm, as determined by micro-CT metrological analysis. GFP-labelled mBMMSCs were mostly located on the compressed side of the periodontal ligament. Compared to the saline group, the number of osteoclasts in the alveolar bone increased significantly (P < 0.01) on the compressed side in the mBMMSC group. Three days after mBMMSC injection, the number of Runx2-GFP double-positive cells on the tension side was significantly higher than that on the compression side. After applying compressive force on the mBMMSCs in vitro for 2 days, RANKL expression was significantly higher than in the non-compression cells, but expression of Alp, Runx2, Ocn and Osterix was significantly decreased (P < 0.05). The numbers of osteoclasts differentiated in response to mBMMs co-cultured with mBMMSCs under pressure load and expression of osteoclast differentiation marker genes (Mmp-9, TRAP and Ctsk) were significantly higher than those in mBMMs stimulated by M-CSF alone (P < 0.05). Conclusions mBMMSCs are not only recruited to the compressed side of the periodontal ligament but can also promote osteoclastogenesis by expressing Rankl, improving the efficiency of OTM.

Evaluation of Effect of Nd:YAG and Er:YAG Lasers on the Periodontal Pathogens: A Systematic Review

2020 ◽  
Author(s):  
Hosein Eslami
Keyword(s):  
Systematic Review ◽  
Chronic Periodontitis ◽  
Complementary Therapy ◽  
Root Surface ◽  
Laser Wavelength ◽  
Time Interval ◽  
Periodontal Pathogens ◽  
Probing Depth ◽  
Treatment Procedures ◽  
The Given

Introduction: By removing the periodontal pathogens and even the invasive bacteria attacking the tissues as well as the lack of normal periodontal treatment procedures, using a laser, as a complementary therapy, can have positive and useful impacts. The present study aims at investigating the impacts of Nd:YAG and Er:YAG laser on periodontal pathogens. Materials and Methods: In this systematic review, articles in the databases of Google Scholar, PubMed and Scopus were searched within 2000-2020. All information is classified into a table. Results: In searching the aforementioned databases and considering the given time interval, 78 articles were found in connection to the searched keywords and abstracts. Once the repeated titles and articles were deleted, 25 studies were selected which were decreased to 16 by the meticulous review by the assessment team. The full text of these 16 articles was reviewed. To make the final choice, 8 articles had the inclusion criteria which included the objectives of this study. Conclusion: To use the laser in treating the chronic periodontitis, an evidence-based study seems to be conducted. A few scientific pieces of evidence are indicating the advantage of a special laser wavelength in the common treatments.Based on most researches reviewed in this study, the results of the complementary application of Nd:YAG and Er:YAG lasers in treating the chronic periodontitis are the better than the ones obtained from the normal teeth scaling and planning the teeth root surface in terms of the reduced probing depth and subgingival bacteria population. The decision was made to use the laser in periodontal surgery based on the advantages such as hemostasis and less pain after the treatment. However, more studies are needed to apply the laser in periodontal treatments.

Sign in / Sign up

Export Citation Format

Share Document