Analysis of gene expression following norepinephrine stimulation in the rat pineal gland using DNA microarray technique

Abstract Noncoding RNAs (ncRNAs), including miRNAs and circRNAs, which are expressed with a daily rhythm in the rat pineal gland, are associated with the regulation of melatonin secretion and other biological functions. However, the mechanisms of these molecules in the rat pineal gland are not yet fully understood. In this study, we found circR-WNK2 was highly expressed at night, which may be involved in the regulation of melatonin secretion through the ceRNA mechanism. By dual luciferase reporter, RNA pulldown, and FISH assays, we found that miR-328a-3p can target circR-WNK2 and the Aa-nat mRNA 3’UTR. Transfection experiments indicated that circR-WNK2 could competitively bind to miR-328a-3p, reduce miR-328a-3p expression, and promote Aa-nat gene expression and melatonin secretion. And by constructing an SCGx rat model, we found that ncRNAs expressed in the pineal gland was regulated by signals from the SCN. This finding supports the hypothesis that these noncoding RNAs may interact to shape the circadian rhythm through transcriptional processing in melatonin synthesis.

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Chronic treatment with dexamethasone alters clock gene expression and melatonin synthesis in rat pineal gland at night

Nature and Science of Sleep ◽

10.2147/nss.s158602 ◽

2018 ◽

Vol Volume 10 ◽

pp. 203-215 ◽

Cited By ~ 3

Author(s):

Daniela Meneses-Santos ◽

Daniella do Carmo Buonfiglio ◽

Rodrigo Antonio Peliciari-Garcia ◽

Angela Maria Ramos-Lobo ◽

Divanízia do Nascimento Souza ◽

...

Keyword(s):

Gene Expression ◽

Pineal Gland ◽

Clock Gene ◽

Chronic Treatment ◽

Melatonin Synthesis ◽

Clock Gene Expression ◽

Rat Pineal Gland

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Identification of circadian gene expression in the rat pineal gland and retina by mRNA differential display

Neuroscience Letters ◽

10.1016/0304-3940(95)11331-p ◽

1995 ◽

Vol 187 (1) ◽

pp. 69-73 ◽

Cited By ~ 10

Author(s):

François Gauer ◽

Wojciech Kedzierski ◽

Cheryl M. Craft

Keyword(s):

Gene Expression ◽

Pineal Gland ◽

Differential Display ◽

Mrna Differential Display ◽

Circadian Gene ◽

Rat Pineal Gland

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Ontogenesis of hydroxyindole-O-methyltransferase gene expression and activity in the rat pineal gland

Developmental Brain Research ◽

10.1016/s0165-3806(98)00114-x ◽

1998 ◽

Vol 110 (2) ◽

pp. 235-239 ◽

Cited By ~ 21

Author(s):

Christophe Ribelayga ◽

François Gauer ◽

Paul Pévet ◽

Valérie Simonneaux

Keyword(s):

Gene Expression ◽

Pineal Gland ◽

Methyltransferase Gene ◽

Rat Pineal Gland ◽

Expression And Activity

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Analysis of daily and circadian gene expression in the rat pineal gland

Neuroscience Research ◽

10.1016/j.neures.2007.10.011 ◽

2008 ◽

Vol 60 (2) ◽

pp. 192-198 ◽

Cited By ~ 14

Author(s):

Chiaki Fukuhara ◽

Gianluca Tosini

Keyword(s):

Gene Expression ◽

Pineal Gland ◽

Circadian Gene ◽

Rat Pineal Gland

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The Potential of 2-aza-8-Oxohypoxanthine as a Cosmetic Ingredient

Cosmetics ◽

10.3390/cosmetics8030060 ◽

2021 ◽

Vol 8 (3) ◽

pp. 60

Author(s):

Hisae Aoshima ◽

Masayuki Ito ◽

Rinta Ibuki ◽

Hirokazu Kawagishi

Keyword(s):

Gene Expression ◽

Cell Proliferation ◽

Microarray Analysis ◽

Dna Microarray ◽

Cell Activation ◽

Skin Barrier ◽

Efficacy Evaluation ◽

Activation Effect ◽

Expression Levels ◽

Dna Microarray Analysis

In this study, we verified the effects of 2-aza-8-oxohypoxanthine (AOH) on human epidermal cell proliferation by performing DNA microarray analysis. Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which measures mitochondrial respiration in normal human epidermal keratinocyte (NHEK) cells. Gene expression levels were determined by DNA microarray analysis of 177 genes involved in skin aging and disease. AOH showed a significant increase in cell viability at concentrations between 7.8 and 31.3 μg/mL and a significant decrease at concentrations above 250 μg/mL. DNA microarray analysis showed that AOH significantly increased the gene expression of CLDN1, DSC1, DSG1, and CDH1 (E-cadherin), which are involved in intercellular adhesion and skin barrier functioning. AOH also up-regulated the expression of KLK5, KLK7, and SPIMK5, which are proteases involved in stratum corneum detachment. Furthermore, AOH significantly stimulated the expression of KRT1, KRT10, TGM1, and IVL, which are considered general differentiation indicators, and that of SPRR1B, a cornified envelope component protein. AOH exerted a cell activation effect on human epidermal cells. Since AOH did not cause cytotoxicity, it was considered that the compound had no adverse effects on the skin. In addition, it was found that AOH stimulated the expression levels of genes involved in skin barrier functioning by DNA microarray analysis. Therefore, AOH has the potential for practical use as a cosmetic ingredient. This is the first report of efficacy evaluation tests performed for AOH.

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