Comparison of surface free energy between reconstructed human epidermis and in situ human skin

2000 ◽  
Vol 6 (4) ◽  
pp. 245-249 ◽  
Author(s):  
G. Lerebour ◽  
S. Cupferman ◽  
C. Cohen ◽  
M. N. Bellon-Fontaine
Keyword(s):  
Free Energy ◽  
Surface Free Energy ◽  
Human Skin ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

scholarly journals In Situ Alkylation of Reconstructed Human Epidermis by Methyl Methanesulfonate: A Quantitative HRMAS NMR Chemical Reactivity Mapping

2020 ◽  
Vol 33 (12) ◽  
pp. 3023-3030
Author(s):  
Hassan Srour ◽  
François-Marie Moussallieh ◽  
Karim Elbayed ◽  
Elena Giménez-Arnau ◽  
Jean-Pierre Lepoittevin
Keyword(s):  
Chemical Reactivity ◽  
Human Epidermis ◽  
Methyl Methanesulfonate ◽  
Reconstructed Human Epidermis ◽  
Hrmas Nmr

Influence of Nanocomposite Surface Coating on Biofilm Formation In Situ

2007 ◽  
Vol 7 (12) ◽  
pp. 4642-4648 ◽  
Author(s):  
M. Hannig ◽  
L. Kriener ◽  
W. Hoth-Hannig ◽  
C. Becker-Willinger ◽  
H. Schmidt
Keyword(s):  
Free Energy ◽  
Composite Coating ◽  
Surface Free Energy ◽  
Biofilm Formation ◽  
Tooth Surface ◽  
Dense Layer ◽  
Electron Microscopic ◽  
Nano Composite ◽  
Nano Composite Coating

Caries and periodontitis, the most wide-spread oral diseases around the world, are caused by bacterial adherence and biofilm formation onto the natural as well as restored tooth surface. One possible way to prevent the pathogenic consequences of intraoral biofilm formation might be the modification of the tooth surface by application of an anti-adhesive coating that interferes with the bacterial attachment and subsequent bacterial accumulation. The objective of this study was to investigate the effect of an experimental, low surface free energy nano-composite coating material on biofilm formation in situ. For this purpose, an organic/inorganic nano-composite coating (NANOMER®, INM, Saarbrücken, Germany) with a surface free energy of 18–20 mJ/m2 was applied to enamel as well as titanium specimens. The nano-composite coated specimens and un-coated controls were attached to removable intraoral splints and carried by volunteers over 24 h in the oral cavity. After intraoral exposure, specimens were processed for transmission electron microscopic analysis. On non-coated enamel and titanium control samples a multi-layer of adherent bacteria was found. In contrast, on nano-composite coated specimens strongly reduced biofilm formation was observed. In most areas of the surface-coated specimens only a 10–20 nm thick electron dense layer of adsorbed salivary proteins with adherent protein agglomerates of 20–80 nm diameter could be detected. In addition, detachment of the adsorbed biofilm from the nano-composite coated surfaces was evident in electron microscopic micrographs. The present investigation provides ultrastructural evidence that it is possible to cover enamel as well as titanium with a nano-composite coating revealing easy-to-clean surface properties that cause reduced biofilm formation and accelerated removal of adherent biofilms under oral conditions.

Permeability of the reconstructed human epidermis model Episkin® in comparison to various human skin preparations☆

2007 ◽  
Vol 66 (1) ◽  
pp. 127-134 ◽  
Author(s):  
Frank Netzlaff ◽  
Monika Kaca ◽  
Udo Bock ◽  
Eleonore Haltner-Ukomadu ◽  
Peter Meiers ◽  
...  
Keyword(s):  
Human Skin ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

Epitaxial BaF2-CaF2 Stacks on Si(111) and Si(100)

1988 ◽  
Vol 116 ◽  
Author(s):  
S. Blunier ◽  
H. Zogg ◽  
H. Weibel
Keyword(s):  
Free Energy ◽  
Surface Free Energy ◽  
Lattice Mismatch ◽  
Lattice Orientation ◽  
Stages Of Growth ◽  
First Time ◽  
Lattice Matched

AbstractStacks of non lattice matched epitaxial CaF2, SrF2 and BaF2 layers have been grown by MBE onto Si(lll), and, for the first time, onto Si(l00). On CaF2 covered Si(lll) surfaces, BaF2 grows in a 2—d way after formation of the first monolayers despite a lattice mismatch of 14%. On Si(l00), BaF2 grows with the same (100)—lattice orientation as the underlying substrateif at least a thin (=100 Å) intermediate CaF2 layer is deposited first. Growth is 3—d on (100)—surfaces because of the large (100)—surface free energy of the group IIa—fluorides. By applying in situ short anneal cyclesat the first stages of growth, the crystallographic quality increases for (111)— as well as for (100)—orientation.

scholarly journals Thyroid-Stimulating Hormone, a Novel, Locally Produced Modulator of Human Epidermal Functions, Is Regulated by Thyrotropin-Releasing Hormone and Thyroid Hormones

Endocrinology ◽  
2010 ◽  
Vol 151 (4) ◽  
pp. 1633-1642 ◽  
Author(s):  
Enikő Bodó ◽  
Benedikt Kany ◽  
Erzsébet Gáspár ◽  
Jana Knüver ◽  
Arno Kromminga ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Human Skin ◽  
Thyroid Stimulating Hormone ◽  
Hair Follicles ◽  
Human Epidermis ◽  
Normal Human Skin ◽  
Skin Cell ◽  
Normal Human ◽  
Scalp Skin

Several elements of the hypothalamic-pituitary-thyroid axis (HPT) reportedly are transcribed by human skin cell populations, and human hair follicles express functional receptors for TSH. Therefore, we asked whether the epidermis of normal human skin is yet another extrathyroidal target of TSH and whether epidermis even produces TSH. If so, we wanted to clarify whether intraepidermal TSH expression is regulated by TRH and/or thyroid hormones and whether TSH alters selected functions of normal human epidermis in situ. TSH and TSH receptor (TSH-R) expression were analyzed in the epidermis of normal human scalp skin by immunohistochemistry and PCR. In addition, full-thickness scalp skin was organ cultured and treated with TSH, TRH, or thyroid hormones, and the effect of TSH treatment on the expression of selected genes was measured by quantitative PCR and/or quantitative immunohistochemistry. Here we show that normal human epidermis expresses TSH at the mRNA and protein levels in situ and transcribes TSH-R. It also contains thyrostimulin transcripts. Intraepidermal TSH immunoreactivity is up-regulated by TRH and down-regulated by thyroid hormones. Although TSH-R immunoreactivity in situ could not be documented within the epidermis, but in the immediately adjacent dermis, TSH treatment of organ-cultured human skin strongly up-regulated epidermal expression of involucrin, loricrin, and keratins 5 and 14. Thus, normal human epidermis in situ is both an extrapituitary source and (possibly an indirect) target of TSH signaling, which regulates defined epidermal parameters. Intraepidermal TSH expression appears to be regulated by the classical endocrine controls that determine the systemic HPT axis.

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