Closed Continuous-flow Centrifuge

Nature ◽  
1968 ◽  
Vol 217 (5131) ◽  
pp. 816-818 ◽  
Author(s):  
GEORGE JUDSON ◽  
ALAN JONES ◽  
ROBERT KELLOGG ◽  
DEAN BUCKNER ◽  
ROBERT EISEL ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Continuous Flow Centrifuge

The Role of Continuous Flow Centrifuge Leucapheresis in the Management of Chronic Lymphocytic Leukaemia

1976 ◽  
Vol 32 (4) ◽  
pp. 609-616 ◽  
Author(s):  
I. E. Fortuny ◽  
D. C. Hadlock ◽  
B. J. Kennedy ◽  
A. Theologides ◽  
J. McCullough
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Continuous Flow ◽  
Lymphocytic Leukaemia ◽  
Continuous Flow Centrifuge

scholarly journals Increased Efficiency of Leukocyte Collection by the Addition of Hydroxyethyl Starch to the Continuous Flow Centrifuge

Blood ◽  
1974 ◽  
Vol 44 (4) ◽  
pp. 571-581 ◽  
Author(s):  
John Milton Mishler ◽  
Daniel C. Hadlock ◽  
Ignacio E. Fortuny ◽  
Robert W. Nicora ◽  
Jeffrey J. McCullough
Keyword(s):  
Hydroxyethyl Starch ◽  
Continuous Flow ◽  
Malignant Disease ◽  
Supportive Therapy ◽  
Control Group ◽  
Input Line ◽  
Healthy Donors ◽  
And Control ◽  
Continuous Flow Centrifuge ◽  
Differential Counts

Abstract A total of 67 leukaphereses were performed with the continuous-flow centrifuge (CFC) on 27 healthy donors for the purpose of obtaining increased yields of granulocytes for infusion into septic patients with acute leukemia accompanied by severe granulocytopenia. The addition of hydroxyethyl starch (HES) to the input line of the CFC significantly (p < 0.005) increased the total number of leukocytes and/or granulocytes collected per donation. A mean yield of 9.72 x 109 and 4.65 x 109 total granulocytes were collected by the HES-treated and control-group donors, respectively. The efficiency of cell collection as evidenced by the total number of leukocytes and/or granulocytes harvested per liter of processed blood was also significantly (p < 0.005) improved by the addition of HES to the continuous-flow centrifuge. Significant alterations in hematologic parameters were not experienced by HES-treated donors undergoing initial and multiple procedures. Pre- and postdonation leukocyte and platelet counts, hemoglobin, prothrombin time, partial thromboplastin time, and leukocyte differential counts were no different whether or not HES was employed for granulocyte collection. The results of the present study demonstrate that increased yields of granulocytes can be harvested by the addition of HES to the continuous-flow centrifuge and made available for supportive therapy to patients experiencing granulocytopenia induced by malignant disease or its treatment.

Systematic Use Of Heparin In The Continuous Flow Centrifuge (CFC) For Blood Cell Separation

1981 ◽  
Author(s):  
M R Morales ◽  
J Pizzuto ◽  
Ma Reyna ◽  
G Castro
Keyword(s):  
Blood Cell ◽  
Continuous Flow ◽  
Cell Separation ◽  
Reliable Method ◽  
Dosage Level ◽  
Thrombin Time ◽  
Coagulation Disorders ◽  
Time Dilution ◽  
Continuous Flow Centrifuge ◽  
Simultaneous Assessment

To date the use of heparin in the CFC has not been adequately controlled, thus exposing donors and patients to coagulation disorders. For this reason, we decided to evaluate the use of heparin by continuous infusion in dosages that would be modified by a simultaneous assessment of its anticoagulant effect, as shown by the thrombin time dilution test (TTDT).The study was performed during 46 leukopher- esis and 27 plasmapheresis. It was ascertained that heparin is an efficient anticoagulant in the CFC, using the TTDT as an effective and reliable method for its control. The initial dose in leukopheresis is one unit per milliliter of blood during the first hour, then half the dose during the next hour, and then a quarter of the dose until the procedure is completed. A TTDT performed every hour will indicate whether the amount of heparin used should be modified. For plasmapheresis, it is neccesary to establish a specific dose in each instance, using the TTDT as described. In most of the subjects, the anticoagulant level was exactly right. There was no case of bleeding or extracorporeal coagulation of the blood.On the basis of these findings, we recommend the use of heparin in the CFC, applying the results of the TTDT as a guide for its dosage level.

The Effect of a Single or Double Dose of Dexamethasone on Granulocyte Collection with the Continuous Flow Centrifuge

Vox Sanguinis ◽  
1975 ◽  
Vol 28 (3) ◽  
pp. 243-248 ◽  
Author(s):  
D. J. Higby ◽  
John Milton Mishler ◽  
W. Rhomberg ◽  
R. W. Nicora ◽  
J. F. Holland
Keyword(s):  
Continuous Flow ◽  
Double Dose ◽  
Continuous Flow Centrifuge

Design and construction of a ventilated cabinet for a continuous flow centrifuge

1974 ◽  
Vol 16 (12) ◽  
pp. 1681-1687 ◽  
Author(s):  
C. G. T. Evans ◽  
R. Harris-Smith ◽  
J. E. D. Stratton ◽  
J. Melling
Keyword(s):  
Continuous Flow ◽  
Continuous Flow Centrifuge ◽  
Design And Construction

Use of heparin for cytapheresis and plasmapheresis in a continuous flow centrifuge

Transfusion ◽  
1982 ◽  
Vol 22 (5) ◽  
pp. 384-387 ◽  
Author(s):  
M Morales ◽  
J Pizzuto ◽  
M Reyna ◽  
R Ambriz ◽  
A Aviles ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Continuous Flow Centrifuge

Fractional Cleanout in a Continuous-Flow Centrifuge

1970 ◽  
Vol 5 (2) ◽  
pp. 137-143 ◽  
Author(s):  
W. K. Sartory
Keyword(s):  
Continuous Flow ◽  
Continuous Flow Centrifuge

A finite difference simulation of a continuous flow centrifuge

1991 ◽  
Vol 93 (3) ◽  
pp. 401-414 ◽  
Author(s):  
J.W. Frederick ◽  
R.J. Ribando ◽  
H.G. Wood
Keyword(s):  
Finite Difference ◽  
Continuous Flow ◽  
Continuous Flow Centrifuge

scholarly journals Effects of temperature on granulocyte preservation

Blood ◽  
1978 ◽  
Vol 52 (2) ◽  
pp. 301-310
Author(s):  
J McCullough ◽  
BJ Wieblen ◽  
PK Peterson ◽  
PG Quie
Keyword(s):  
Continuous Flow ◽  
Storage Conditions ◽  
Chemotactic Response ◽  
Granulocyte Transfusion ◽  
Bacterial Killing ◽  
Effects Of Temperature ◽  
Continuous Flow Centrifuge

With the increasing use of granulocyte transfusion it is becoming important to determine if granulocytes can be preserved for a few days. If so, the optimum storage conditions must be identified. We studied the function in vitro of granulocytes collected as they would be for transfusion by continuous-flow centrifuge leukapheresis (CFCL) and filtration leukapheresis (FL). Granulocytes collected by CFCL maintained normal ability to phagocytose and kill bacteria after 48 hr and normal chemotaxis after 24 hr of storage at 20 degrees--24 degrees C. Neither 1 degrees--6 degrees C nor 37 degrees C were as effective in preserving chemotactic response. Agitation of the granulocyte suspension during storage caused reduced bacterial killing and chemotaxis. Granulocytes collected by FL functioned very poorly after 24 hr storage at all temperatures studied. These studies suggest that it may be possible to store CFCL granulocytes at 20 degrees--24 degrees C for 24 hr. FL granulocytes should not be stored at all.

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