scholarly journals The role of primary tumour sidedness, EGFR gene copy number and EGFR promoter methylation in RAS/BRAF wild-type colorectal cancer patients receiving irinotecan/cetuximab

2017 ◽  
Vol 117 (3) ◽  
pp. 315-321 ◽  
Author(s):  
Laura Demurtas ◽  
Marco Puzzoni ◽  
Riccardo Giampieri ◽  
Pina Ziranu ◽  
Valeria Pusceddu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Copy Number ◽  
Primary Tumour ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Wild Type ◽  
Egfr Gene ◽  
Colorectal Cancer Patients

Epidermal growth factor gene amplification is not frequent and cannot account for antitumor activity of cetuximab plus chemotherapy in advanced colorectal cancer patients

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 3561-3561 ◽  
Author(s):  
C. Garufi ◽  
M. Mottolese ◽  
A. Cianciulli ◽  
M. Zeuli ◽  
S. Buglioni ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Antitumor Activity ◽  
Cancer Patients ◽  
Copy Number ◽  
Advanced Colorectal Cancer ◽  
Gene Copy Number ◽  
Chromosome 7 ◽  
Gene Copy ◽  
Egfr Gene ◽  
Colorectal Cancer Patients

3561 Cetuximab has been shown to be active in the metastatic colorectal cancer, but EGFR detection by immunohistochemistry is not predictive for tumor response. Moroni et al (Lancet Oncology 2005) showed that, in patients responsive to Cetuximab, EGFR gene copy number, assessed by FISH, was increased. On this basis, copy number and protein status of EGFR were investigated in 70 primary and/or metastatic colorectal carcinomas. Protein expression was assessed by immunohistochemistry (IHC) using DAKO EGFRPharmDX kit. Gene and chromosome 7 copy numbers were identified by fluorescent in situ hybridization (FISH). Dual-target, dual-color FISH assays were performed using the LSI EGFR SpectrumOrange/CEP 7 Spectrum Green probe. EGFR gene copy number, chromosome 7 copy number and the average EGFR gene to chromosome 7 signal ratio were reported as FISH genetic variables. Chromosome 7 was polysomic when cancer cells showed multiple centromere signals: low polysomy (2 to 5 signals), high polysomy (>5 signals). Samples with a ratio value ≥ 2.0 were considered to be amplified. EGFR protein was overexpressed in 57 out of 70 patients (81%). In the group of 58 patients evaluated as polysomic, 48 (82,7%) had a low polysomy level, whereas 20 (12.7%) had a high polysomy level. Gene amplification was seen only in 3/70 patients. High polysomy was evidenced only in the group of patients displaying an EGFR IHC score 2+/3+. Forty-six pretreated patients received a cetuximab-based treatment. Response to treatment has been already evaluated in 26 FISH-negative patients while for the other 20 it is still too early. We observed 7 PR (27%), 9 SD (35%) and 10 PD (38%). This study shows that in this series of advanced colorectal cancer patients, EGFR amplification, measured by FISH, is a rare event (4%) and could be considered together with chromosome 7 polysomy for the antitumor activity. No significant financial relationships to disclose.

Cetuximab Shows Activity in Colorectal Cancer Patients With Tumors for Which FISH Analysis Does Not Detect an Increase in EGFR Gene Copy Number

2007 ◽  
Vol 15 (2) ◽  
pp. 649-654 ◽  
Author(s):  
Antoine Italiano ◽  
Philippe Follana ◽  
François-Xavier Caroli ◽  
Jean-Luc Badetti ◽  
Daniel Benchimol ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Fish Analysis ◽  
Egfr Gene ◽  
Colorectal Cancer Patients

Association between EGFR gene copy number and KRAS status and impact on outcome prediction in colorectal cancer patients treated with cetuximab

2008 ◽  
Vol 26 (15_suppl) ◽  
pp. 11093-11093 ◽  
Author(s):  
N. Personeni ◽  
H. Piessevaux ◽  
S. Fieuws ◽  
F. Di Fiore ◽  
W. De Roock ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Copy Number ◽  
Outcome Prediction ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Egfr Gene ◽  
Kras Status ◽  
Colorectal Cancer Patients

scholarly journals Topoisomerase 1(TOP1 ) gene copy number in stage III colorectal cancer patients and its relation to prognosis

2012 ◽  
Vol 7 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Maria Unni Rømer ◽  
Sune Boris Nygård ◽  
Ib Jarle Christensen ◽  
Signe Lykke Nielsen ◽  
Kirsten Vang Nielsen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Copy Number ◽  
Gene Copy Number ◽  
Stage Iii ◽  
Gene Copy ◽  
Topoisomerase 1 ◽  
Colorectal Cancer Patients

EGFR gene copy number to predict response to anti-EGFR treatment and survival in RAS wild type and RAS/BRAF/PIK3CA wild type metastatic colorectal cancer.

2016 ◽  
Vol 34 (15_suppl) ◽  
pp. e15108-e15108
Author(s):  
Olli Carpen ◽  
Annika Ålgars ◽  
Jari Sundström ◽  
Soili Kytölä ◽  
Pia Österlund ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Wild Type ◽  
Egfr Gene
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