Evaluation of a Double-Tracer Autoradiographic Technique for the Measurement of Both Local Cerebral Glucose Metabolism and Local Cerebral Blood Flow
1985 ◽
Vol 5
(2)
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pp. 335-337
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Keyword(s):
A solvent washout technique is evaluated that could be used to remove one 14C tracer after a combined autoradiographic CMRglu and CBF study using [14C]2-deoxyglucose (2DG) and [14C]iodoantipyrine (IAP). The technique permits the simultaneous measurement of local CBF and local CMRglu in the same animal without the use of the short-lived tracers of iodine, 123I and 131I, for iodoantipyrine labeling. This report shows that brain tissue sections did not retain 14C from [14C]2DG when chloroform was used as the washout solvent. Chloroform washing removes nearly all the 14C from [14C]IAP. For this study, careful attention was given to the control, pre-wash measurement of 14C concentration.
1984 ◽
Vol 4
(1)
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pp. 88-95
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1994 ◽
Vol 16
(4)
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pp. 289-296
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Keyword(s):
2020 ◽
Vol Publish Ahead of Print
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Keyword(s):
Keyword(s):