Organization of vesicular trafficking in epithelia

2005 ◽  
Vol 6 (3) ◽  
pp. 233-247 ◽  
Author(s):  
Enrique Rodriguez-Boulan ◽  
Geri Kreitzer ◽  
Anne Müsch
Keyword(s):  
Vesicular Trafficking

scholarly journals Rab1-AMPylation by Legionella DrrA is allosterically activated by Rab1

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jiqing Du ◽  
Marie-Kristin von Wrisberg ◽  
Burak Gulen ◽  
Matthias Stahl ◽  
Christian Pett ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Adenosine Monophosphate ◽  
Vesicular Trafficking ◽  
Bacterial Protein ◽  
Post Translational Modification ◽  
Allosteric Control ◽  
Rab Protein ◽  
Biochemical Characterisation ◽  
A Site ◽  
Insight Into

AbstractLegionella pneumophila infects eukaryotic cells by forming a replicative organelle – the Legionella containing vacuole. During this process, the bacterial protein DrrA/SidM is secreted and manipulates the activity and post-translational modification (PTM) states of the vesicular trafficking regulator Rab1. As a result, Rab1 is modified with an adenosine monophosphate (AMP), and this process is referred to as AMPylation. Here, we use a chemical approach to stabilise low-affinity Rab:DrrA complexes in a site-specific manner to gain insight into the molecular basis of the interaction between the Rab protein and the AMPylation domain of DrrA. The crystal structure of the Rab:DrrA complex reveals a previously unknown non-conventional Rab-binding site (NC-RBS). Biochemical characterisation demonstrates allosteric stimulation of the AMPylation activity of DrrA via Rab binding to the NC-RBS. We speculate that allosteric control of DrrA could in principle prevent random and potentially cytotoxic AMPylation in the host, thereby perhaps ensuring efficient infection by Legionella.

scholarly journals High Content Study of Vesicular Trafficking in Polarized Cells

2012 ◽  
Vol 102 (3) ◽  
pp. 194a
Author(s):  
Javier Mazzaferri ◽  
Stephane Lefrançois ◽  
Santiago Costantino
Keyword(s):  
Vesicular Trafficking ◽  
Polarized Cells

Analyses of Proteins Involved in Vesicular Trafficking in Platelets of Mouse Models of Hermansky Pudlak Syndrome

1999 ◽  
Vol 68 (1) ◽  
pp. 14-23 ◽  
Author(s):  
Beverly Richards-Smith ◽  
Edward K. Novak ◽  
Elliott K. Jang ◽  
Ping He ◽  
Richard J. Haslam ◽  
...  
Keyword(s):  
Mouse Models ◽  
Vesicular Trafficking ◽  
Hermansky Pudlak Syndrome

scholarly journals Vesicular trafficking in characean green algae and the possible involvement of a VAMP72-family protein

2014 ◽  
Vol 9 (4) ◽  
pp. e28466 ◽  
Author(s):  
Marion C Hoepflinger ◽  
Christina Hametner ◽  
Takashi Ueda ◽  
Ilse Foissner
Keyword(s):  
Green Algae ◽  
Vesicular Trafficking ◽  
Family Protein

scholarly journals Retromer has a selective function in cargo sorting via endosome transport carriers

2018 ◽  
Vol 218 (2) ◽  
pp. 615-631 ◽  
Author(s):  
Yi Cui ◽  
Julian M. Carosi ◽  
Zhe Yang ◽  
Nicholas Ariotti ◽  
Markus C. Kerr ◽  
...  
Keyword(s):  
Vesicular Trafficking ◽  
Lysosomal Hydrolases ◽  
Cell Level ◽  
Ultrastructural Alteration ◽  
Membrane Protein Complex ◽  
Lysosomal Function ◽  
Trafficking Pathway ◽  
Peripheral Membrane Protein ◽  
And Function ◽  
Proteolytic Capacity

Retromer is a peripheral membrane protein complex that coordinates multiple vesicular trafficking events within the endolysosomal system. Here, we demonstrate that retromer is required for the maintenance of normal lysosomal morphology and function. The knockout of retromer subunit Vps35 causes an ultrastructural alteration in lysosomal structure and aberrant lysosome function, leading to impaired autophagy. At the whole-cell level, knockout of retromer Vps35 subunit reduces lysosomal proteolytic capacity as a consequence of the improper processing of lysosomal hydrolases, which is dependent on the trafficking of the cation-independent mannose 6-phosphate receptor (CI-M6PR). Incorporation of CI-M6PR into endosome transport carriers via a retromer-dependent process is restricted to those tethered by GCC88 but not golgin-97 or golgin-245. Finally, we show that this retromer-dependent retrograde cargo trafficking pathway requires SNX3, but not other retromer-associated cargo binding proteins, such as SNX27 or SNX-BAR proteins. Therefore, retromer does contribute to the retrograde trafficking of CI-M6PR required for maturation of lysosomal hydrolases and lysosomal function.

scholarly journals Identification and Characterization of Genes Required for Cell-to-Cell Fusion in Neurospora crassa

2011 ◽  
Vol 10 (8) ◽  
pp. 1100-1109 ◽  
Author(s):  
Ci Fu ◽  
Priyadarshini Iyer ◽  
Amrita Herkal ◽  
Julia Abdullah ◽  
Angela Stout ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Transcription Factors ◽  
Neurospora Crassa ◽  
Cell Fusion ◽  
Single Gene ◽  
Vesicular Trafficking ◽  
Screening Procedure ◽  
Fusion Genes ◽  
Fusion Event ◽  
Screening Experiments

ABSTRACT A screening procedure was used to identify cell fusion (hyphal anastomosis) mutants in the Neurospora crassa single gene deletion library. Mutants with alterations in 24 cell fusion genes required for cell fusion between conidial anastomosis tubes (CATs) were identified and characterized. The cell fusion genes identified included 14 genes that are likely to function in signal transduction pathways needed for cell fusion to occur ( mik-1 , mek-1 , mak-1 , nrc-1 , mek-2 , mak-2 , rac-1 , pp2A , so/ham-1 , ham-2 , ham-3 , ham-5 , ham-9 , and mob3 ). The screening experiments also identified four transcription factors that are required for cell fusion ( adv-1 , ada-3 , rco-1 , and snf5 ). Three genes encoding proteins likely to be involved in the process of vesicular trafficking were also identified as needed for cell fusion during the screening ( amph-1 , ham-10 , pkr1 ). Three of the genes identified by the screening procedure, ham-6 , ham-7 , and ham-8 , encode proteins that might function in mediating the plasma membrane fusion event. Three of the putative signal transduction proteins, three of the transcription factors, the three putative vesicular trafficking proteins, and the three proteins that might function in mediating cell fusion had not been identified previously as required for cell fusion.

scholarly journals Structural basis for acyl chain control over glycosphingolipid sorting and vesicular trafficking

2021 ◽  
Author(s):  
Stefanie S. Schmieder ◽  
Raju Tatituri ◽  
Michael Anderson ◽  
Kate Kelly ◽  
Wayne I. Lencer
Keyword(s):  
Acyl Chain ◽  
Vesicular Trafficking ◽  
Structural Motif ◽  
Structural Basis ◽  
Membrane Organization ◽  
Endosomal Sorting ◽  
Lysosomal Sorting ◽  
Acyl Chains ◽  
Complex Sphingolipids ◽  
Endocytic Pathways

AbstractThe complex sphingolipids exhibit a diversity of ceramide acyl chain structures that influence their trafficking and intracellular distributions, but how the cell discerns among the different ceramides to affect such sorting remains unknown. To address mechanism, we synthesized a library of GM1 glycosphingolipids with naturally varied acyl chains and quantitatively assessed their sorting among different endocytic pathways. We found that a stretch of at least 14 saturated carbons extending from C1 at the water-bilayer interface dictated lysosomal sorting by exclusion from endosome sorting tubules. Sorting to the lysosome by the C14*-motif was cholesterol dependent. Perturbations of the C14*-motif by unsaturation enabled GM1 entry into endosomal sorting tubules of the recycling and retrograde pathways independently of cholesterol. Unsaturation occurring beyond the C14*-motif in very long acyl chains rescued lysosomal sorting. These results define a structural motif underlying membrane organization of sphingolipids and implicate cholesterol-sphingolipid nanodomain formation in sorting mechanisms.

scholarly journals Participation of intracellular vesicular trafficking in acute pancreatitis

Suizo ◽  
2018 ◽  
Vol 33 (4) ◽  
pp. 723-729
Author(s):  
Kenichi TAKAHASHI ◽  
Hirosato MASHIMA ◽  
Hirohide OHNISHI
Keyword(s):  
Acute Pancreatitis ◽  
Vesicular Trafficking

Protrudin and sphingolipid regulate neuronal function via vesicular trafficking

2009 ◽  
Vol 65 ◽  
pp. S38
Author(s):  
Michiko Shirane
Keyword(s):  
Vesicular Trafficking ◽  
Neuronal Function

Fibronectin impedes “myelin” sheet-directed flow in oligodendrocytes: A role for a beta 1 integrin-mediated PKC signaling pathway in vesicular trafficking

2006 ◽  
Vol 33 (2) ◽  
pp. 150-159 ◽  
Author(s):  
Zuzana Šišková ◽  
Wia Baron ◽  
Hans de Vries ◽  
Dick Hoekstra
Keyword(s):  
Signaling Pathway ◽  
Vesicular Trafficking ◽  
Directed Flow ◽  
Pkc Signaling ◽  
Beta 1 Integrin
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