scholarly journals New Scanning Electron Microscopic Method for Determination of Adipocyte Size in Humans and Mice*

Obesity ◽  
2007 ◽  
Vol 15 (7) ◽  
pp. 1657-1665 ◽  
Author(s):  
Wen Guo ◽  
Sherman Bigornia ◽  
Ilit Leizerman ◽  
Weisheng Xie ◽  
Marie McDonnell ◽  
...  
Author(s):  
Betty I. Tarnowski ◽  
Gregory R. Schonbaum

Neither light microscopy nor transmission electron microscopy lend themselves to an accurate assessment of focal changes of epithelium: both techniques are limited by sampling procedures. The same limitation, however, does not apply to scanning electron microscopy (SEM) (1,2) which permits statistically meaningful analyses on a larger number of samples. The usefulness of such an approach was explored in our studies on the induction of damage to rat urothelium by cyclophosphamide (3,4) and its prevention by adjunct therapy with 2,3-dimercaptopropane sulfonate (DMPS). Portions of the bladder were sampled from the dome, central region and trigone and the tissue was prepared for SEM by dehydration in acetone, followed by critical point drying and gold coating. SEM analyses were performed in a two-step procedure using a Novascan scanning electron microscope at low (20X) and high (100X) magnifications.


2013 ◽  
Vol 468 ◽  
pp. 32-35
Author(s):  
Xiao Long Li ◽  
Guo Zhong Li

In the experiment, polystyrene particles, cement, fly ash, activator and fibers were used as raw material to prepare new cement-based composite insulation board by compression molding. Through the determination of best content of fly ash, the effects of fly ash on strength of composite insulation board were studied. Through the determination of the optimal content of activators, the effects of activators on the mechanical properties of composite insulation board were studied. Through the determination of the optimal dosage of fibers, the effects of fibers on performance of composite insulation board were studied. The morphology in the samples was observed by scanning electron microscopic, and the action mechanism of each component was studied.


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