scholarly journals MdATG18a overexpression improves basal thermotolerance in transgenic apple by decreasing damage to chloroplasts

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Liuqing Huo ◽  
Xun Sun ◽  
Zijian Guo ◽  
Xin Jia ◽  
Runmin Che ◽  
...  
Keyword(s):  
Basal Thermotolerance ◽  
Transgenic Apple

scholarly journals MdVQ37 overexpression reduces basal thermotolerance in transgenic apple by affecting transcription factor activity and salicylic acid homeostasis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Qinglong Dong ◽  
Dingyue Duan ◽  
Wenqian Zheng ◽  
Dong Huang ◽  
Qian Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salicylic Acid ◽  
Plant Growth ◽  
Stress Factors ◽  
Regulatory Function ◽  
Transcription Factor Activity ◽  
Factor Activity ◽  
Transgenic Lines ◽  
Basal Thermotolerance ◽  
Transgenic Apple

AbstractHigh temperature (HT) is one of the most important environmental stress factors and seriously threatens plant growth, development, and production. VQ motif-containing proteins are transcriptional regulators that have been reported to regulate plant growth and developmental processes, including responses to biotic and abiotic stresses. However, the relationships between VQ motif-containing proteins and HT stress have not been studied in depth in plants. In this study, transgenic apple (Malus domestica) plants overexpressing the apple VQ motif-containing protein-coding gene (MdVQ37) were exposed to HT stress, and the transgenic lines exhibited a heat-sensitive phenotype. In addition, physiological and biochemical studies revealed that, compared with WT plants, transgenic lines had lower enzymatic activity and photosynthetic capacity and lower amounts of nonenzymatic antioxidant system metabolites under HT stress. Transcriptome analysis revealed 1379 genes whose expression differed between the transgenic lines and WT plants. GO and KEGG pathway analyses showed that transcription factor activity and plant hormone signaling pathways were differentially influenced and enriched in the transgenic lines. Salicylic acid (SA) content analysis indicated that overexpression of MdVQ37 reduced the content of endogenous SA by regulating the expression of SA catabolism-related genes, which ultimately resulted in disruption of the SA-dependent signaling pathway under HT stress. The application of SA slightly increased the survival rate of the transgenic lines under HT stress. Taken together, our results indicate that apple MdVQ37 has a regulatory function in basal thermotolerance by modulating the activity of transcription factors and SA homeostasis. Overall, this study provides novel insights that improve our understanding of the various functions of VQ motif-containing proteins.

scholarly journals Chitinase activities, scab resistance, mycorrhization rates and biomass of own-rooted and grafted transgenic apple

2012 ◽  
Vol 35 (2) ◽  
pp. 466-473 ◽  
Author(s):  
Tina Schäfer ◽  
Magda-Viola Hanke ◽  
Henryk Flachowsky ◽  
Stephan König ◽  
Andreas Peil ◽  
...  
Keyword(s):  
Scab Resistance ◽  
Transgenic Apple

scholarly journals Melatonin promotes adventitious root formation in apple by promoting the function of MdWOX11

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jiangping Mao ◽  
Chundong Niu ◽  
Ke Li ◽  
Shiyue Chen ◽  
Muhammad Mobeen Tahir ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Zeatin Riboside ◽  
Adventitious Root Formation ◽  
Control Group ◽  
Apple Rootstocks ◽  
Phenotypic Analysis ◽  
Malus Prunifolia ◽  
Treatment Groups ◽  
Transgenic Lines ◽  
Transgenic Apple

Abstract Background Melatonin (MT) is important for plant growth and development; however, it is not known whether MT is involved in apple adventitious root (AR) development. In this study, we treated Malus prunifolia (MP) at four different stages of AR development, and analyzed the level of the endogenous hormones MT, auxin (IAA), zeatin-riboside (ZR), abscisic acid (ABA), and gibberellins (GA1 + 3) in all four treatment groups and the untreated control group. The expression of MT, IAA biosynthesis, transport and signal transduction, the cell cycle, and root development related genes were quantified by RT-qPCR. The function of MdWOX11 was analyzed in transgenic apple plants. Results The promotion of AR development by MT was dependent on the stage of AR induction between 0 and 2 d in apple rootstocks. MT-treatment increased the level of IAA and crosstalk existed between MT and IAA during AR formation. The expression of MdWOX11 was induced by MT treatment and positively regulated AR formation in apple. Furthermore, transgenic lines that overexpressed MdWOX11 lines produced more ARs than ‘GL3’. Phenotypic analysis indicated that MdWOX11 overexpression lines were more sensitive to exogenous MT treatment than ‘GL3’, suggesting that MdWOX11 regulates AR formation in response to MT in apple rootstock. Conclusions MT promotes AR formation mainly during the AR induction stage by inducing IAA levels and upregulating MdWOX11.

scholarly journals The Apple Autophagy-Related Gene MdATG9 Confers Tolerance to Low Nitrogen in Transgenic Apple Callus

2020 ◽  
Vol 11 ◽  
Author(s):  
Liuqing Huo ◽  
Zijian Guo ◽  
Zhijun Zhang ◽  
Xin Jia ◽  
Yiming Sun ◽  
...  
Keyword(s):  
Related Gene ◽  
Low Nitrogen ◽  
Transgenic Apple
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