scholarly journals Net decrease in spine-surface GluA1-containing AMPA receptors after post-learning sleep in the adult mouse cortex

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Daisuke Miyamoto ◽  
William Marshall ◽  
Giulio Tononi ◽  
Chiara Cirelli
Keyword(s):  
Ampa Receptors ◽  
Motor Task ◽  
Relative Advantage ◽  
Two Photon ◽  
Adult Mice ◽  
Mature Brain ◽  
Mouse Cortex ◽  
Before And After ◽  
Receptor Plasticity

AbstractThe mechanisms by which sleep benefits learning and memory remain unclear. Sleep may further strengthen the synapses potentiated by learning or promote broad synaptic weakening while protecting the newly potentiated synapses. We tested these ideas by combining a motor task whose consolidation is sleep-dependent, a marker of synaptic AMPA receptor plasticity, and repeated two-photon imaging to track hundreds of spines in vivo with single spine resolution. In mouse motor cortex, sleep leads to an overall net decrease in spine-surface GluA1-containing AMPA receptors, both before and after learning. Molecular changes in single spines during post-learning sleep are correlated with changes in performance after sleep. The spines in which learning leads to the largest increase in GluA1 expression have a relative advantage after post-learning sleep compared to sleep deprivation, because sleep weakens all remaining spines. These results are obtained in adult mice, showing that sleep-dependent synaptic down-selection also benefits the mature brain.

scholarly journals Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging

2021 ◽  
pp. 0271678X2110685
Author(s):  
Stephanie K Bonney ◽  
Liam T Sullivan ◽  
Timothy J Cherry ◽  
Richard Daneman ◽  
Andy Y Shih
Keyword(s):  
Cell Types ◽  
Structural Features ◽  
Main Trunk ◽  
Perivascular Niche ◽  
Two Photon ◽  
Mural Cells ◽  
Adult Mice ◽  
Photon Imaging ◽  
Two Photon Imaging

Perivascular fibroblasts (PVFs) are recognized for their pro-fibrotic role in many central nervous system disorders. Like mural cells, PVFs surround blood vessels and express Pdgfrβ. However, these shared attributes hinder the ability to distinguish PVFs from mural cells. We used in vivo two-photon imaging and transgenic mice with PVF-targeting promoters (Col1a1 or Col1a2) to compare the structure and distribution of PVFs and mural cells in cerebral cortex of healthy, adult mice. We show that PVFs localize to all cortical penetrating arterioles and their offshoots (arteriole-capillary transition zone), as well as the main trunk of only larger ascending venules. However, the capillary zone is devoid of PVF coverage. PVFs display short-range mobility along the vessel wall and exhibit distinct structural features (flattened somata and thin ruffled processes) not seen with smooth muscle cells or pericytes. These findings clarify that PVFs and mural cells are distinct cell types coexisting in a similar perivascular niche.

In vivo two-photon uncaging of glutamate revealing the structure–function relationships of dendritic spines in the neocortex of adult mice

2011 ◽  
Vol 71 ◽  
pp. e206
Author(s):  
Jun Noguchi ◽  
Akira Nagaoka ◽  
Satoshi Watanabe ◽  
Graham C.R. Ellis-Davies ◽  
Kazuo Kitamura ◽  
...  
Keyword(s):  
Structure Function ◽  
Dendritic Spines ◽  
Two Photon ◽  
Adult Mice

scholarly journals Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Austin R Graves ◽  
Richard H Roth ◽  
Han L Tan ◽  
Qianwen Zhu ◽  
Alexei M Bygrave ◽  
...  
Keyword(s):  
Ampa Receptors ◽  
Large Scale ◽  
Optical Probe ◽  
Neuronal Communication ◽  
Two Photon ◽  
Dynamic Expression ◽  
Behavioral Paradigm ◽  
Technological Barriers ◽  
Synapse Detection

Elucidating how synaptic molecules such as AMPA receptors mediate neuronal communication and tracking their dynamic expression during behavior is crucial to understand cognition and disease, but current technological barriers preclude large-scale exploration of molecular dynamics in vivo. We have developed a suite of innovative methodologies that break through these barriers: a new knockin mouse line with fluorescently tagged endogenous AMPA receptors, two-photon imaging of hundreds of thousands of labeled synapses in behaving mice, and computer-vision-based automatic synapse detection. Using these tools, we can longitudinally track how the strength of populations of synapses changes during behavior. We used this approach to generate an unprecedentedly detailed spatiotemporal map of synapses undergoing changes in strength following sensory experience. More generally, these tools can be used as an optical probe capable of measuring functional synapse strength across entire brain areas during any behavioral paradigm, describing complex system-wide changes with molecular precision.

scholarly journals Stringent structural plasticity of dendritic spines revealed by two-photon glutamate uncaging in adult mouse neocortex in vivo

2019 ◽  
Author(s):  
Jun Noguchi ◽  
Akira Nagaoka ◽  
Tatsuya Hayama ◽  
Hasan Ucar ◽  
Sho Yagishita ◽  
...  
Keyword(s):  
Time Course ◽  
Hippocampal Slices ◽  
Low Frequency ◽  
Structural Plasticity ◽  
Two Photon ◽  
Low Magnesium ◽  
Adult Mice ◽  
First Time

AbstractTwo-photon uncaging of glutamate is widely utilized to characterize structural plasticity in brain slice preparations in vitro. In this study, we investigated spine plasticity by using, for the first time, glutamate uncaging in the neocortex of adult mice in vivo. Spine enlargement was successfully induced in a smaller fraction of spines in the neocortex (22%) than in young hippocampal slices (95%), even under a low magnesium condition. Once induced, the time course and mean amplitudes of long-term enlargement were the same (81%) as those in vitro. However, low-frequency (1–2 Hz) glutamate uncaging caused spine shrinkage in a similar fraction (34%) of spines as in vitro, but spread to the neighboring spines less frequently than in vitro. Thus, we found that structural plasticity can occur similarly in the adult neocortex in vivo as in the hippocampus in vitro, although it happens stringently in a smaller subset of spines.

scholarly journals In vivo Two-Photon Imaging of Anesthesia-Specific Alterations in Microglial Surveillance and Photodamage-Directed Motility in Mouse Cortex

2019 ◽  
Vol 13 ◽  
Author(s):  
Weilun Sun ◽  
Kunimichi Suzuki ◽  
Dmytro Toptunov ◽  
Stoyan Stoyanov ◽  
Michisuke Yuzaki ◽  
...  
Keyword(s):  
Two Photon ◽  
Mouse Cortex ◽  
Directed Motility ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging

2021 ◽  
Author(s):  
Stephanie K Bonney ◽  
Liam T Sullivan ◽  
Timothy Joel Cherry ◽  
Richard Daneman ◽  
Andy Y Shih
Keyword(s):  
Cell Types ◽  
Structural Features ◽  
Main Trunk ◽  
Perivascular Niche ◽  
Two Photon ◽  
Mural Cells ◽  
Adult Mice ◽  
Photon Imaging ◽  
Two Photon Imaging

Perivascular fibroblasts (PVFs) are recognized for their pro-fibrotic role in many central nervous system disorders. Like mural cells, PVFs surround blood vessels and express Pdgfrβ. However, these shared attributes hinder the ability to distinguish PVFs from mural cells. We used in vivo two-photon imaging and transgenic mice with PVF-targeting promoters (Col1a1 or Col1a2) to compare the structure and distribution of PVFs and mural cells in cerebral cortex of healthy, adult mice. We show that PVFs localize to all cortical penetrating arterioles and their pre-capillary offshoots, as well as the main trunk of only larger ascending venules. However, the capillary zone is devoid of PVF coverage. PVFs display short-range mobility along the vessel wall and exhibit distinct structural features (flattened somata and thin ruffled processes) not seen with smooth muscle cells or pericytes. These findings clarify that PVFs and mural cells are distinct cell types coexisting in a similar perivascular niche.

Sign in / Sign up

Export Citation Format

Share Document