scholarly journals 4D polycarbonates via stereolithography as scaffolds for soft tissue repair

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Andrew C. Weems ◽  
Maria C. Arno ◽  
Wei Yu ◽  
Robert T. R. Huckstepp ◽  
Andrew P. Dove
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Healing Process ◽  
Surface Erosion ◽  
Tissue Engineering Scaffolds ◽  
Adipose Tissue Engineering ◽  
Filling Materials ◽  
Aliphatic Polycarbonate ◽  
Soft Tissue Engineering

Abstract3D printing has emerged as one of the most promising tools to overcome the processing and morphological limitations of traditional tissue engineering scaffold design. However, there is a need for improved minimally invasive, void-filling materials to provide mechanical support, biocompatibility, and surface erosion characteristics to ensure consistent tissue support during the healing process. Herein, soft, elastomeric aliphatic polycarbonate-based materials were designed to undergo photopolymerization into supportive soft tissue engineering scaffolds. The 4D nature of the printed scaffolds is manifested in their shape memory properties, which allows them to fill model soft tissue voids without deforming the surrounding material. In vivo, adipocyte lobules were found to infiltrate the surface-eroding scaffold within 2 months, and neovascularization was observed over the same time. Notably, reduced collagen capsule thickness indicates that these scaffolds are highly promising for adipose tissue engineering and repair.

Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds

Biomaterials ◽  
2004 ◽  
Vol 25 (27) ◽  
pp. 5857-5866 ◽  
Author(s):  
Richard M. Day ◽  
Aldo R. Boccaccini ◽  
Sandra Shurey ◽  
Judith A. Roether ◽  
Alastair Forbes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Bioactive Glass ◽  
Polyglycolic Acid ◽  
Tissue Engineering Scaffolds ◽  
Polyglycolic Acid Mesh ◽  
Soft Tissue Engineering

Polymer structure-property requirements for stereolithographic 3D printing of soft tissue engineering scaffolds

Biomaterials ◽  
2017 ◽  
Vol 140 ◽  
pp. 170-188 ◽  
Author(s):  
Ryan J. Mondschein ◽  
Akanksha Kanitkar ◽  
Christopher B. Williams ◽  
Scott S. Verbridge ◽  
Timothy E. Long
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
3D Printing ◽  
Polymer Structure ◽  
Structure Property ◽  
Tissue Engineering Scaffolds ◽  
Soft Tissue Engineering

scholarly journals Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering

2017 ◽  
Vol 8 ◽  
pp. 204173141774415 ◽  
Author(s):  
Lukas Kessler ◽  
Sandra Gehrke ◽  
Marc Winnefeld ◽  
Birgit Huber ◽  
Eva Hoch ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Adipogenic Differentiation ◽  
Building Blocks ◽  
Fatty Acid Binding ◽  
Angiogenic Potential ◽  
Adipose Tissue Engineering ◽  
Angiogenesis Assay ◽  
Soft Tissue Engineering

In vitro–generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose–derived stem cells (hASCs). ASCs were incorporated into methacrylated gelatin/hyaluronan hydrogels. The gels were photocrosslinked with a lithium phenyl-2,4,6-trimethylbenzoylphosphinate photoinitiator and analyzed for cell viability and adipogenic differentiation of ASCs over a period of 30 days. Additionally, an angiogenesis assay was performed to assess their angiogenic potential. After 24 h, ASCs showed increased viability on composite hydrogels. These results were consistent over 21 days of culture. By induction of adipogenic differentiation, the mature adipocytes were observed after 7 days of culture, their number significantly increased until day 28 as well as expression of fatty acid binding protein 4 and adiponectin. Our scaffolds are promising as building blocks for adipose tissue engineering and allowed long viability, proliferation, and differentiation of ASCs.

Biodegradable polyurethane/graphene oxide scaffolds for soft tissue engineering: in vivo behavior assessment

2019 ◽  
Vol 69 (17) ◽  
pp. 1101-1111 ◽  
Author(s):  
Aleksandra Ivanoska-Dacikj ◽  
Gordana Bogoeva-Gaceva ◽  
Andres Krumme ◽  
Elvira Tarasova ◽  
Chiara Scalera ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Soft Tissue ◽  
Behavior Assessment ◽  
Biodegradable Polyurethane ◽  
Soft Tissue Engineering

3D-Printable Biodegradable Polyester Tissue Scaffolds for Cell Adhesion

2015 ◽  
Vol 68 (9) ◽  
pp. 1409 ◽  
Author(s):  
Justin M. Sirrine ◽  
Allison M. Pekkanen ◽  
Ashley M. Nelson ◽  
Nicholas A. Chartrain ◽  
Christopher B. Williams ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Glycol Adipate ◽  
Tissue Engineering Scaffolds ◽  
Ethylene Glycol Adipate ◽  
Biodegradable Poly ◽  
Soft Tissue Engineering ◽  
Tissue Scaffolding

Additive manufacturing, or three-dimensional (3D) printing, has emerged as a viable technique for the production of vascularized tissue engineering scaffolds. In this report, a biocompatible and biodegradable poly(tri(ethylene glycol) adipate) dimethacrylate was synthesized and characterized for suitability in soft-tissue scaffolding applications. The polyester dimethacrylate exhibited highly efficient photocuring, hydrolyzability, and 3D printability in a custom microstereolithography system. The photocured polyester film demonstrated significantly improved cell attachment and viability as compared with controls. These results indicate promise of novel, printable polyesters for 3D patterned, vascularized soft-tissue engineering scaffolds.

scholarly journals Apigenin Induces Browning in White Adipocytes Mediated by VEGF-PRDM16 Signaling.

2022 ◽  
Author(s):  
Sreelekshmi Sreeku ◽  
Vinu Vijayan ◽  
Fathe Singh ◽  
Manu Sudhakar ◽  
Kiran M S
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
De Novo ◽  
Metabolic Shift ◽  
Tissue Engineering Scaffolds ◽  
White Adipocyte ◽  
White Adipocytes ◽  
Soft Tissue Engineering ◽  
Treatment Of Obesity ◽  
Endothelial Growth Factor

Abstract The white adipose tissues are metabolically inert which results in deranged biological signalling disorders resulting in obesity. Lack of vascularisation in these tissues is mainly responsible to make them metabolically inert. Not much work has been done in this direction to understand the role of angiogenesis in white adipocytes metabolism. In the present study, we evaluated the effect of angiogenic modulator in modulating the metabolism in white adipocyte. Nutraceuticals apigenin (Apg) was employed as angiogenic modulator. The results indicated that promoting angiogenesis by Apg enhanced the de novo differentiation and trans-differentiation of white adipocyte into brown like phenotype by triggering vascular endothelial growth factor A. Cross talk between endothelial and adipocytes were observed in co-culture studies. The metabolic shift in white adipocytes was observed to be due to the upregulation of PRDM16 cascade. The study provides new insights for inducing metabolic shift in white adipocytes by modulation of angiogenesis in white adipocyte to trigger browning for the treatment of obesity. Further the study opens scopes for development of medical devices for obese subjects, an area that needs to be addressed specifically with reference to soft tissue engineering as commercial soft tissue engineering scaffolds does not suit the obese patients.

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