Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds

Additive manufacturing, or three-dimensional (3D) printing, has emerged as a viable technique for the production of vascularized tissue engineering scaffolds. In this report, a biocompatible and biodegradable poly(tri(ethylene glycol) adipate) dimethacrylate was synthesized and characterized for suitability in soft-tissue scaffolding applications. The polyester dimethacrylate exhibited highly efficient photocuring, hydrolyzability, and 3D printability in a custom microstereolithography system. The photocured polyester film demonstrated significantly improved cell attachment and viability as compared with controls. These results indicate promise of novel, printable polyesters for 3D patterned, vascularized soft-tissue engineering scaffolds.

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4D polycarbonates via stereolithography as scaffolds for soft tissue repair

Nature Communications ◽

10.1038/s41467-021-23956-6 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Andrew C. Weems ◽

Maria C. Arno ◽

Wei Yu ◽

Robert T. R. Huckstepp ◽

Andrew P. Dove

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Healing Process ◽

Surface Erosion ◽

Tissue Engineering Scaffolds ◽

Adipose Tissue Engineering ◽

Filling Materials ◽

Aliphatic Polycarbonate ◽

Soft Tissue Engineering

Abstract3D printing has emerged as one of the most promising tools to overcome the processing and morphological limitations of traditional tissue engineering scaffold design. However, there is a need for improved minimally invasive, void-filling materials to provide mechanical support, biocompatibility, and surface erosion characteristics to ensure consistent tissue support during the healing process. Herein, soft, elastomeric aliphatic polycarbonate-based materials were designed to undergo photopolymerization into supportive soft tissue engineering scaffolds. The 4D nature of the printed scaffolds is manifested in their shape memory properties, which allows them to fill model soft tissue voids without deforming the surrounding material. In vivo, adipocyte lobules were found to infiltrate the surface-eroding scaffold within 2 months, and neovascularization was observed over the same time. Notably, reduced collagen capsule thickness indicates that these scaffolds are highly promising for adipose tissue engineering and repair.

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Apigenin Induces Browning in White Adipocytes Mediated by VEGF-PRDM16 Signaling.

10.21203/rs.3.rs-1210533/v1 ◽

2022 ◽

Author(s):

Sreelekshmi Sreeku ◽

Vinu Vijayan ◽

Fathe Singh ◽

Manu Sudhakar ◽

Kiran M S

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

De Novo ◽

Metabolic Shift ◽

Tissue Engineering Scaffolds ◽

White Adipocyte ◽

White Adipocytes ◽

Soft Tissue Engineering ◽

Treatment Of Obesity ◽

Endothelial Growth Factor

Abstract The white adipose tissues are metabolically inert which results in deranged biological signalling disorders resulting in obesity. Lack of vascularisation in these tissues is mainly responsible to make them metabolically inert. Not much work has been done in this direction to understand the role of angiogenesis in white adipocytes metabolism. In the present study, we evaluated the effect of angiogenic modulator in modulating the metabolism in white adipocyte. Nutraceuticals apigenin (Apg) was employed as angiogenic modulator. The results indicated that promoting angiogenesis by Apg enhanced the de novo differentiation and trans-differentiation of white adipocyte into brown like phenotype by triggering vascular endothelial growth factor A. Cross talk between endothelial and adipocytes were observed in co-culture studies. The metabolic shift in white adipocytes was observed to be due to the upregulation of PRDM16 cascade. The study provides new insights for inducing metabolic shift in white adipocytes by modulation of angiogenesis in white adipocyte to trigger browning for the treatment of obesity. Further the study opens scopes for development of medical devices for obese subjects, an area that needs to be addressed specifically with reference to soft tissue engineering as commercial soft tissue engineering scaffolds does not suit the obese patients.

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Electrospun PCL/PGS Composite Fibers Incorporating Bioactive Glass Particles for Soft Tissue Engineering Applications

Nanomaterials ◽

10.3390/nano10050978 ◽

2020 ◽

Vol 10 (5) ◽

pp. 978 ◽

Cited By ~ 4

Author(s):

Marina Luginina ◽

Katharina Schuhladen ◽

Roberto Orrú ◽

Giacomo Cao ◽

Aldo R. Boccaccini ◽

...

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Bioactive Glass ◽

Fiber Diameter ◽

Diameter Distribution ◽

Electrospun Fibers ◽

Average Fiber Diameter ◽

Composite Fibers ◽

Engineering Applications ◽

Soft Tissue Engineering

Poly(glycerol-sebacate) (PGS) and poly(epsilon caprolactone) (PCL) have been widely investigated for biomedical applications in combination with the electrospinning process. Among others, one advantage of this blend is its suitability to be processed with benign solvents for electrospinning. In this work, the suitability of PGS/PCL polymers for the fabrication of composite fibers incorporating bioactive glass (BG) particles was investigated. Composite electrospun fibers containing silicate or borosilicate glass particles (13-93 and 13-93BS, respectively) were obtained and characterized. Neat PCL and PCL composite electrospun fibers were used as control to investigate the possible effect of the presence of PGS and the influence of the bioactive glass particles. In fact, with the addition of PGS an increase in the average fiber diameter was observed, while in all the composite fibers, the presence of BG particles induced an increase in the fiber diameter distribution, without changing significantly the average fiber diameter. Results confirmed that the blended fibers are hydrophilic, while the addition of BG particles does not affect fiber wettability. Degradation test and acellular bioactivity test highlight the release of the BG particles from all composite fibers, relevant for all applications related to therapeutic ion release, i.e., wound healing. Because of weak interface between the incorporated BG particles and the polymeric fibers, mechanical properties were not improved in the composite fibers. Promising results were obtained from preliminary biological tests for potential use of the developed mats for soft tissue engineering applications.

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