scholarly journals Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mitchell Clough ◽  
Ichun Anderson Chen ◽  
Seong-Wook Park ◽  
Allison M. Ahrens ◽  
Jeffrey N. Stirman ◽  
...  
Keyword(s):  
Brain Function ◽  
Brain Regions ◽  
Two Photon ◽  
High Speeds ◽  
Trade Offs ◽  
Acquisition Speed ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Global Brain ◽  
The Brain

AbstractUnderstanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

scholarly journals Flexible Simultaneous Mesoscale Two-Photon Imaging of Neural Activity at High Speeds

2021 ◽  
Author(s):  
Mitchell Clough ◽  
Ichen Anderson Chen ◽  
Seong-Wook Park ◽  
Allison M Ahrens ◽  
Jeffrey N Stirman ◽  
...  
Keyword(s):  
Brain Function ◽  
Brain Regions ◽  
Two Photon ◽  
High Speeds ◽  
Trade Offs ◽  
Acquisition Speed ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Global Brain ◽  
The Brain

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

Automated long-term two-photon imaging in head-fixed walking Drosophila

2021 ◽  
Author(s):  
Andres Flores Valle ◽  
Rolf Honnef ◽  
Johannes D. Seelig
Keyword(s):  
Structural Changes ◽  
Long Term Memory ◽  
Head Direction ◽  
Multiple Timescales ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Millisecond Range ◽  
The Brain

The brain of Drosophila shows dynamics at multiple timescales, from the millisecond range of fast voltage or calcium transients to functional and structural changes occurring over multiple days. To relate such dynamics to behavior requires monitoring neural circuits across these multiple timescales in behaving animals. Here, we develop a technique for automated long-term two-photon imaging in fruit flies, during wakefulness and sleep, navigating in virtual reality over up to seven days. The method is enabled by laser surgery, a microrobotic arm for controlling forceps for dissection assistance, an automated feeding robot, as well as volumetric, simultaneous multiplane imaging. The approach is validated in the fly's head direction system. Imaging in behaving flies over multiple timescales will be useful for understanding circadian activity, learning and long-term memory, or sleep.

scholarly journals Transparent arrays of bilayer-nanomesh microelectrodes for simultaneous electrophysiology and two-photon imaging in the brain

2018 ◽  
Vol 4 (9) ◽  
pp. eaat0626 ◽  
Author(s):  
Yi Qiang ◽  
Pietro Artoni ◽  
Kyung Jin Seo ◽  
Stanislav Culaclii ◽  
Victoria Hogan ◽  
...  
Keyword(s):  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
The Brain

scholarly journals Synaptic Structure and Function in the Mouse Somatosensory Cortex during Chronic Pain:In VivoTwo-Photon Imaging

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Sun Kwang Kim ◽  
Kei Eto ◽  
Junichi Nabekura
Keyword(s):  
Somatosensory Cortex ◽  
Structure And Function ◽  
Imaging Studies ◽  
Two Photon ◽  
Synaptic Structure ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
And Function ◽  
The Brain

Recent advances in two-photon microscopy and fluorescence labeling techniques have enabled us to directly see the structural and functional changes in neurons and glia, and even at synapses, in the brain of living animals. Long-termin vivotwo-photon imaging studies have shown that some postsynaptic dendritic spines in the adult cortex are rapidly eliminated or newly generated, in response to altered sensory input or synaptic activity, resulting in experience/activity-dependent rewiring of neuronal circuits.In vivoCa2+imaging studies have revealed the distinct, input-specific response patterns of excitatory neurons in the brain. These updatedin vivoapproaches are just beginning to be used for the study of pathophysiological mechanisms of chronic diseases. In this paper, we introduce recentin vivotwo-photon imaging studies demonstrating how plastic changes in synaptic structure and function of the mouse somatosensory cortex, following peripheral injury, contribute to chronic pain conditions, like neuropathic and inflammatory pain.

scholarly journals Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Simon Chamberland ◽  
Helen H Yang ◽  
Michael M Pan ◽  
Stephen W Evans ◽  
Sihui Guan ◽  
...  
Keyword(s):  
Random Access ◽  
Two Photon ◽  
Organotypic Slice Cultures ◽  
Voltage Transients ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Low Sensitivity ◽  
Voltage Dynamics ◽  
The Brain ◽  
Voltage Indicator

Monitoring voltage dynamics in defined neurons deep in the brain is critical for unraveling the function of neuronal circuits but is challenging due to the limited performance of existing tools. In particular, while genetically encoded voltage indicators have shown promise for optical detection of voltage transients, many indicators exhibit low sensitivity when imaged under two-photon illumination. Previous studies thus fell short of visualizing voltage dynamics in individual neurons in single trials. Here, we report ASAP2s, a novel voltage indicator with improved sensitivity. By imaging ASAP2s using random-access multi-photon microscopy, we demonstrate robust single-trial detection of action potentials in organotypic slice cultures. We also show that ASAP2s enables two-photon imaging of graded potentials in organotypic slice cultures and in Drosophila. These results demonstrate that the combination of ASAP2s and fast two-photon imaging methods enables detection of neural electrical activity with subcellular spatial resolution and millisecond-timescale precision.

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