In vivo two-photon imaging of neuronal circuit activity using transgenic mice that express fluorescent calcium sensor proteins in the brain

2011 ◽  
Vol 71 ◽  
pp. e309
Author(s):  
Masaaki Sato ◽  
Masako Kawamo ◽  
Tanvir Islam ◽  
Yoko Yamaguchi ◽  
Masamichi Ohkura ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Calcium Sensor ◽  
Neuronal Circuit ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Sensor Proteins ◽  
The Brain ◽  
Calcium Sensor Proteins

scholarly journals In Vivo Two-Photon Imaging of Astrocytes in GFAP-GFP Transgenic Mice

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0170005 ◽  
Author(s):  
Dongjun Guo ◽  
Jia Zou ◽  
Nicholas Rensing ◽  
Michael Wong
Keyword(s):  
Transgenic Mice ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Synaptic Structure and Function in the Mouse Somatosensory Cortex during Chronic Pain:In VivoTwo-Photon Imaging

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Sun Kwang Kim ◽  
Kei Eto ◽  
Junichi Nabekura
Keyword(s):  
Somatosensory Cortex ◽  
Structure And Function ◽  
Imaging Studies ◽  
Two Photon ◽  
Synaptic Structure ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
And Function ◽  
The Brain

Recent advances in two-photon microscopy and fluorescence labeling techniques have enabled us to directly see the structural and functional changes in neurons and glia, and even at synapses, in the brain of living animals. Long-termin vivotwo-photon imaging studies have shown that some postsynaptic dendritic spines in the adult cortex are rapidly eliminated or newly generated, in response to altered sensory input or synaptic activity, resulting in experience/activity-dependent rewiring of neuronal circuits.In vivoCa2+imaging studies have revealed the distinct, input-specific response patterns of excitatory neurons in the brain. These updatedin vivoapproaches are just beginning to be used for the study of pathophysiological mechanisms of chronic diseases. In this paper, we introduce recentin vivotwo-photon imaging studies demonstrating how plastic changes in synaptic structure and function of the mouse somatosensory cortex, following peripheral injury, contribute to chronic pain conditions, like neuropathic and inflammatory pain.

scholarly journals In vivo two-photon imaging of T cell motility in joint-draining lymph nodes in a mouse model of rheumatoid arthritis

2012 ◽  
Vol 278 (1-2) ◽  
pp. 158-165 ◽  
Author(s):  
Tamás Kobezda ◽  
Sheida Ghassemi-Nejad ◽  
Tibor T. Glant ◽  
Katalin Mikecz
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
Mouse Model ◽  
Lymph Nodes ◽  
Cell Motility ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Draining Lymph Nodes

scholarly journals In Vivo Two-Photon Imaging of Neurons and Glia in the Mouse Spinal Cord

2012 ◽  
Vol 2012 (12) ◽  
pp. pdb.prot072264-pdb.prot072264 ◽  
Author(s):  
H. Steffens ◽  
F. Nadrigny ◽  
F. Kirchhoff
Keyword(s):  
Spinal Cord ◽  
Mouse Spinal Cord ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Kilohertz full-frame two-photon imaging of cortical hemodynamics in vivo

2021 ◽  
Author(s):  
Guanghan Meng ◽  
Jiang Lan Fan ◽  
Qinrong Zhang ◽  
Kevin K. Tsia ◽  
Na Ji
Keyword(s):  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Flexible Simultaneous Mesoscale Two-Photon Imaging of Neural Activity at High Speeds

2021 ◽  
Author(s):  
Mitchell Clough ◽  
Ichen Anderson Chen ◽  
Seong-Wook Park ◽  
Allison M Ahrens ◽  
Jeffrey N Stirman ◽  
...  
Keyword(s):  
Brain Function ◽  
Brain Regions ◽  
Two Photon ◽  
High Speeds ◽  
Trade Offs ◽  
Acquisition Speed ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Global Brain ◽  
The Brain

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

Organotypic cell cultures and two-photon imaging: Tools for in vitro and in vivo assessment of percutaneous drug delivery and skin toxicity

2012 ◽  
Vol 161 (2) ◽  
pp. 656-667 ◽  
Author(s):  
Sari Pappinen ◽  
Evgeny Pryazhnikov ◽  
Leonard Khiroug ◽  
Marica B. Ericson ◽  
Marjo Yliperttula ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Cultures ◽  
Skin Toxicity ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Percutaneous Drug Delivery ◽  
In Vivo Assessment

scholarly journals Ultralow Impedance Graphene Microelectrodes with High Optical Transparency for Simultaneous Deep Two-Photon Imaging in Transgenic Mice

2018 ◽  
Vol 28 (31) ◽  
pp. 1800002 ◽  
Author(s):  
Yichen Lu ◽  
Xin Liu ◽  
Ryoma Hattori ◽  
Chi Ren ◽  
Xingwang Zhang ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Optical Transparency ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging
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