scholarly journals Optical deciphering of multinary chiral compound mixtures through organic reaction based chemometric chirality sensing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Diandra S. Hassan ◽  
Christian Wolf
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Scientific Progress ◽  
Chiral Chromatography ◽  
Chiral Compound ◽  
High Throughput Analysis ◽  
Physical Separation ◽  
Waste Production ◽  
High Throughput Experimentation ◽  
Operational Simplicity

AbstractThe advances of high-throughput experimentation technology and chemometrics have revolutionized the pace of scientific progress and enabled previously inconceivable discoveries, in particular when used in tandem. Here we show that the combination of chirality sensing based on small-molecule optical probes that bind to amines and amino alcohols via dynamic covalent or click chemistries and powerful chemometric tools that achieve orthogonal data fusion and spectral deconvolution yields a streamlined multi-modal sensing protocol that allows analysis of the absolute configuration, enantiomeric composition and concentration of structurally analogous—and therefore particularly challenging—chiral target compounds without laborious and time-consuming physical separation. The practicality, high accuracy, and speed of this approach are demonstrated with complicated quaternary and octonary mixtures of varying chemical and chiral compositions. The advantages over chiral chromatography and other classical methods include operational simplicity, increased speed, reduced waste production, low cost, and compatibility with multiwell plate technology if high-throughput analysis of hundreds of samples is desired.

Nanomole-scale high-throughput chemistry for the synthesis of complex molecules

Science ◽  
2014 ◽  
Vol 347 (6217) ◽  
pp. 49-53 ◽  
Author(s):  
Alexander Buitrago Santanilla ◽  
Erik L. Regalado ◽  
Tony Pereira ◽  
Michael Shevlin ◽  
Kevin Bateman ◽  
...  
Keyword(s):  
High Throughput ◽  
Scale Up ◽  
Natural Product Synthesis ◽  
Test Case ◽  
Coupling Reactions ◽  
High Throughput Analysis ◽  
Complex Molecules ◽  
Cross Coupling Reactions ◽  
High Throughput Experimentation ◽  
Palladium Catalyzed

At the forefront of new synthetic endeavors, such as drug discovery or natural product synthesis, large quantities of material are rarely available and timelines are tight. A miniaturized automation platform enabling high-throughput experimentation for synthetic route scouting to identify conditions for preparative reaction scale-up would be a transformative advance. Because automated, miniaturized chemistry is difficult to carry out in the presence of solids or volatile organic solvents, most of the synthetic “toolkit” cannot be readily miniaturized. Using palladium-catalyzed cross-coupling reactions as a test case, we developed automation-friendly reactions to run in dimethyl sulfoxide at room temperature. This advance enabled us to couple the robotics used in biotechnology with emerging mass spectrometry–based high-throughput analysis techniques. More than 1500 chemistry experiments were carried out in less than a day, using as little as 0.02 milligrams of material per reaction.

Low-cost dedicated mini-arrays for high-throughput analysis of DNA copy-number alterations in neuroblastoma

2008 ◽  
Vol 269 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Katleen De Preter ◽  
Björn Menten ◽  
Sara De Brouwer ◽  
Candy Kumps ◽  
Evi Michels ◽  
...  
Keyword(s):  
High Throughput ◽  
Copy Number ◽  
Low Cost ◽  
Copy Number Alterations ◽  
Dna Copy Number ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Dna Copy Number Alterations

scholarly journals Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion

2021 ◽  
Author(s):  
Marcelo S. Conzentino ◽  
Tatielle P. C. Santos ◽  
Khaled A. Selim ◽  
Berenike Wagner ◽  
Janette T. Alford ◽  
...  
Keyword(s):  
High Throughput ◽  
Magnetic Beads ◽  
Low Cost ◽  
Magnetic Bead ◽  
Minimal Amount ◽  
High Throughput Analysis ◽  
Major Drawback ◽  
Analytical Time ◽  
Magnetic Extraction ◽  
Bead Immunoassay

ABSTRACTA technique allowing high throughput, fast and low-cost quantitative analysis of human IgG antibodies reacting to SARS-CoV-2 antigens will be required to understand the levels of protecting antibodies in the population raised in response to infections and/or to immunization. We described previously a fast, simple, and inexpensive Ni2+ magnetic bead immunoassay which allowed detection of human antibodies reacting against the SARS-CoV-2 nucleocapsid protein using a minimal amount of serum or blood. A major drawback of the previously described system was that it only processed 12 samples simultaneously. Here we describe a manually operating inexpensive 96 well plate magnetic extraction / homogenization process which allows high throughput analysis delivering results of 96 samples in chromogenic format in 12 minutes or in fluorescent ultrafast format which takes only 7 minutes. We also show that His tag antigen purification can be performed on the fly while loading antigens to the Ni2+ magnetic beads in a process which takes only 12 min reducing the pre analytical time and cost. Finally, we show that the magnetic bead immunoassay is antigen flexible and can be performed using either Nucleocapsid, Spike or Spike RBD. The method performed with low inter and intra assay variability using different antigens and detection modes and was able to deliver >99.5% specificity and >95% sensitivity for a cohort of 203 pre pandemic and 63 COVID-19 positive samples.

scholarly journals High throughput analysis enables high throughput experimentation in pharmaceutical process research

2019 ◽  
Vol 4 (11) ◽  
pp. 1895-1911 ◽  
Author(s):  
Christopher J. Welch
Keyword(s):  
High Throughput ◽  
Process Research ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
High Throughput Experimentation

High throughput experimentation has become widely used in the discovery and development of new medicines.

FreeLoader: An Open Source Universal Testing Machine for High-Throughput Experimentation

2011 ◽  
Author(s):  
John R. Amend ◽  
Hod Lipson
Keyword(s):  
Open Source ◽  
High Throughput ◽  
Low Cost ◽  
Testing Machine ◽  
Universal Testing Machine ◽  
Universal Testing ◽  
High Throughput Experimentation ◽  
Compressive Loads ◽  
Order Of Magnitude ◽  
The Cost

We present a low cost, desktop size, open source, universal testing machine, designed for inexpensive high-throughput material testing. The tester can apply tensile and compressive loads up to 5 kN at rates ranging from 2 mm/min to 30 mm/min. Force measurements are achieved with ±1.8 N accuracy. The parts list for this machine represents an order of magnitude reduction in the cost per testing unit as compared to commercial systems. We describe the design and construction of the tester and validate its performance. The design, parts list, control software, and user manual are made available freely online under the open source BSD license.

scholarly journals Cycling gradient capillary electrophoresis: A low-cost tool for high-throughput analysis of genetic variations

2003 ◽  
Vol 24 (11) ◽  
pp. 1716-1722 ◽  
Author(s):  
Marek Minarik ◽  
Lucie Minarikova ◽  
Jens Bjørheim ◽  
Per Olaf Ekstrøm
Keyword(s):  
Capillary Electrophoresis ◽  
High Throughput ◽  
Low Cost ◽  
Genetic Variations ◽  
High Throughput Analysis ◽  
Throughput Analysis

Combination of Free Software, Light Microscopy and Non-specific Dyes for a High-throughput Analysis in Live/Dead Cell Count

2015 ◽  
Vol 11 (4) ◽  
pp. 233-238 ◽  
Author(s):  
Luciano Cardoso ◽  
Suellen Cordeiro ◽  
Marcio Fronza ◽  
Denise Endringer ◽  
Tadeu de Andrade ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Cell Count ◽  
High Throughput ◽  
Free Software ◽  
Dead Cell ◽  
High Throughput Analysis ◽  
Throughput Analysis

Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell–ECM Interactions

2021 ◽  
Author(s):  
Ruoxing Lei ◽  
Erin A. Akins ◽  
Kelly C. Y. Wong ◽  
Nicole A. Repina ◽  
Kayla J. Wolf ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Analysis ◽  
Throughput Analysis
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