scholarly journals Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion

2021 ◽  
Author(s):  
Marcelo S. Conzentino ◽  
Tatielle P. C. Santos ◽  
Khaled A. Selim ◽  
Berenike Wagner ◽  
Janette T. Alford ◽  
...  
Keyword(s):  
High Throughput ◽  
Magnetic Beads ◽  
Low Cost ◽  
Magnetic Bead ◽  
Minimal Amount ◽  
High Throughput Analysis ◽  
Major Drawback ◽  
Analytical Time ◽  
Magnetic Extraction ◽  
Bead Immunoassay

ABSTRACTA technique allowing high throughput, fast and low-cost quantitative analysis of human IgG antibodies reacting to SARS-CoV-2 antigens will be required to understand the levels of protecting antibodies in the population raised in response to infections and/or to immunization. We described previously a fast, simple, and inexpensive Ni2+ magnetic bead immunoassay which allowed detection of human antibodies reacting against the SARS-CoV-2 nucleocapsid protein using a minimal amount of serum or blood. A major drawback of the previously described system was that it only processed 12 samples simultaneously. Here we describe a manually operating inexpensive 96 well plate magnetic extraction / homogenization process which allows high throughput analysis delivering results of 96 samples in chromogenic format in 12 minutes or in fluorescent ultrafast format which takes only 7 minutes. We also show that His tag antigen purification can be performed on the fly while loading antigens to the Ni2+ magnetic beads in a process which takes only 12 min reducing the pre analytical time and cost. Finally, we show that the magnetic bead immunoassay is antigen flexible and can be performed using either Nucleocapsid, Spike or Spike RBD. The method performed with low inter and intra assay variability using different antigens and detection modes and was able to deliver >99.5% specificity and >95% sensitivity for a cohort of 203 pre pandemic and 63 COVID-19 positive samples.

scholarly journals A magnetic bead immunoassay to detect human IgG reactive to SARS-CoV-2 Spike S1 RBD produced in Escherichia coli

2021 ◽  
Author(s):  
Marcelo dos Santos Conzentino ◽  
Ana C Goncalves ◽  
NIgella M Paula ◽  
Fabiane G Rego ◽  
Dalila Zanette ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Receptor Binding ◽  
High Throughput ◽  
Low Cost ◽  
Magnetic Bead ◽  
Binding Domain ◽  
Receptor Binding Domain ◽  
Operating Characteristics ◽  
Human Igg ◽  
Bead Immunoassay

Immunological assays to detect SARS-CoV-2 Spike receptor binding domain antigen seroconversion in humans are important tools to monitor the levels of protecting antibodies in the population in response to infection and/or immunization. Here we describe a simple, low cost and high throughput Ni2+ magnetic bead immunoassay to detect human IgG reactive to Spike S1 RBD Receptor Binding Domain produced in Escherichia coli. A 6xHis tagged Spike S1 RBD was expressed in E. coli and purified by affinity chromatography. The protein was mobilized on the surface of Ni2+ magnetic beads and used to investigate the presence of reactive IgG in the serum obtained from pre-pandemic and COVID-19 confirmed cases. The method was validated with a cohort of 290 samples and an area under the receiver operating characteristics curve of 0.94 was obtained. The method operated with>82% sensitivity at 98% specificity and was also able to track human IgG raised in response to vaccination with Comirnaty with 85% sensitivity. The IgG signal obtained with the described method was well correlated with the signal obtained when pre fusion Spike produced in HEK cell lines were used as antigen. This novel low-cost and high throughput immunoassay may act as an important tool to investigate protecting IgG antibodies against SARS-CoV-2 in the human population.

A low cost and high throughput magnetic bead-based immuno-agglutination assay in confined droplets

Lab on a Chip ◽  
2013 ◽  
Vol 13 (12) ◽  
pp. 2344 ◽  
Author(s):  
Bruno Teste ◽  
Anaïs Ali-Cherif ◽  
Jean Louis Viovy ◽  
Laurent Malaquin
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Magnetic Bead ◽  
Agglutination Assay

Low-cost dedicated mini-arrays for high-throughput analysis of DNA copy-number alterations in neuroblastoma

2008 ◽  
Vol 269 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Katleen De Preter ◽  
Björn Menten ◽  
Sara De Brouwer ◽  
Candy Kumps ◽  
Evi Michels ◽  
...  
Keyword(s):  
High Throughput ◽  
Copy Number ◽  
Low Cost ◽  
Copy Number Alterations ◽  
Dna Copy Number ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Dna Copy Number Alterations

scholarly journals Optical deciphering of multinary chiral compound mixtures through organic reaction based chemometric chirality sensing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Diandra S. Hassan ◽  
Christian Wolf
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Scientific Progress ◽  
Chiral Chromatography ◽  
Chiral Compound ◽  
High Throughput Analysis ◽  
Physical Separation ◽  
Waste Production ◽  
High Throughput Experimentation ◽  
Operational Simplicity

AbstractThe advances of high-throughput experimentation technology and chemometrics have revolutionized the pace of scientific progress and enabled previously inconceivable discoveries, in particular when used in tandem. Here we show that the combination of chirality sensing based on small-molecule optical probes that bind to amines and amino alcohols via dynamic covalent or click chemistries and powerful chemometric tools that achieve orthogonal data fusion and spectral deconvolution yields a streamlined multi-modal sensing protocol that allows analysis of the absolute configuration, enantiomeric composition and concentration of structurally analogous—and therefore particularly challenging—chiral target compounds without laborious and time-consuming physical separation. The practicality, high accuracy, and speed of this approach are demonstrated with complicated quaternary and octonary mixtures of varying chemical and chiral compositions. The advantages over chiral chromatography and other classical methods include operational simplicity, increased speed, reduced waste production, low cost, and compatibility with multiwell plate technology if high-throughput analysis of hundreds of samples is desired.

scholarly journals Cycling gradient capillary electrophoresis: A low-cost tool for high-throughput analysis of genetic variations

2003 ◽  
Vol 24 (11) ◽  
pp. 1716-1722 ◽  
Author(s):  
Marek Minarik ◽  
Lucie Minarikova ◽  
Jens Bjørheim ◽  
Per Olaf Ekstrøm
Keyword(s):  
Capillary Electrophoresis ◽  
High Throughput ◽  
Low Cost ◽  
Genetic Variations ◽  
High Throughput Analysis ◽  
Throughput Analysis

Combination of Free Software, Light Microscopy and Non-specific Dyes for a High-throughput Analysis in Live/Dead Cell Count

2015 ◽  
Vol 11 (4) ◽  
pp. 233-238 ◽  
Author(s):  
Luciano Cardoso ◽  
Suellen Cordeiro ◽  
Marcio Fronza ◽  
Denise Endringer ◽  
Tadeu de Andrade ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Cell Count ◽  
High Throughput ◽  
Free Software ◽  
Dead Cell ◽  
High Throughput Analysis ◽  
Throughput Analysis

Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell–ECM Interactions

2021 ◽  
Author(s):  
Ruoxing Lei ◽  
Erin A. Akins ◽  
Kelly C. Y. Wong ◽  
Nicole A. Repina ◽  
Kayla J. Wolf ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Analysis ◽  
Throughput Analysis

Development of pattern recognition based on the nanosheet-DNA probes and an extendible DNA library

The Analyst ◽  
2021 ◽  
Author(s):  
Jiawei Qi ◽  
Pinhua Rao ◽  
Lele Wang ◽  
Li Xu ◽  
Yanli Wen ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
High Throughput ◽  
Analysis Data ◽  
Dna Probes ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Dna Library ◽  
Array Sensing

Pattern recognition, also called “array sensing” is a recognition strategy with a wide and expandable analysis range, based on the high-throughput analysis data. In this work, we constructed a sensor...

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

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