Cryo-EM structure of the
            Blastochloris viridis LH1–RC complex at 2.9 Å

A novel Gram-negative, motile, bacteriochlorophyll b-containing purple non-sulfur bacterium, strain JA248T, was isolated from phototrophic enrichments of a yellow–green epilithic biofilm sample collected from Gulmarg, India. The genomic DNA G+C content of strain JA248T was 63.8 mol%. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain JA248T had highest similarity to members of the genus Blastochloris and was closely related to Blastochloris sulfoviridis DSM 729T (98.5 % sequence similarity) and Blastochloris viridis DSM 133T (98.4 %) of the class Alphaproteobacteria. Strain JA248T was characterized based on polyphasic taxonomy, and distinct phenotypic and molecular differences based on DNA–DNA hybridization (relatedness of <46.5 % with the two species of the genus Blastochloris), multilocus sequence analysis, and phenotypic and chemotaxonomic evidence separated strain JA248T from other species of the genus Blastochloris. Strain JA248T therefore represents a novel species in the genus Blastochloris, for which the name Blastochloris gulmargensis sp. nov. is proposed. The type strain is JA248T ( = JCM 14795T = DSM 19786T).

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Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways

Scientific Reports ◽

10.1038/srep09741 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 11

Author(s):

Yusuke Tsukatani ◽

Jiro Harada ◽

Jiro Nomata ◽

Haruki Yamamoto ◽

Yuichi Fujita ◽

...

Keyword(s):

Rhodobacter Sphaeroides ◽

Biosynthetic Pathways ◽

Blastochloris Viridis

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Mapping lipid and detergent molecules at the surface of membrane proteins

Biochemical Society Transactions ◽

10.1042/bst0390775 ◽

2011 ◽

Vol 39 (3) ◽

pp. 775-779 ◽

Cited By ~ 4

Author(s):

Richard J. Cogdell ◽

Alastair T. Gardiner ◽

Aleksander W. Roszak ◽

Sigitas Stončius ◽

Pavel Kočovský ◽

...

Keyword(s):

Membrane Proteins ◽

Crystal Structures ◽

Present Article ◽

Electron Density ◽

Rhodobacter Sphaeroides ◽

Binding Sites ◽

Heavy Atom ◽

Hydrophobic Surface ◽

Density Maps ◽

Blastochloris Viridis

Electron-density maps for the crystal structures of membrane proteins often show features suggesting binding of lipids and/or detergent molecules on the hydrophobic surface, but usually it is difficult to identify the bound molecules. In our studies, heavy-atom-labelled phospholipids and detergents have been used to unequivocally identify these binding sites at the surfaces of test membrane proteins, the reaction centres from Rhodobacter sphaeroides and Blastochloris viridis. The generality of this method is discussed in the present article.

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Revised Genome Sequence of the Purple Photosynthetic Bacterium Blastochloris viridis

Genome Announcements ◽

10.1128/genomea.01520-15 ◽

2016 ◽

Vol 4 (1) ◽

Cited By ~ 3

Author(s):

Lu-Ning Liu ◽

Matthew Faulkner ◽

Xuan Liu ◽

Fang Huang ◽

Alistair C. Darby ◽

...

Keyword(s):

Genetic Engineering ◽

Genome Sequence ◽

Photosynthetic Bacterium ◽

Purple Bacterium ◽

Metabolic Versatility ◽

Purple Photosynthetic Bacterium ◽

Blastochloris Viridis

Blastochloris viridis is a unique anaerobic, phototrophic purple bacterium that produces bacteriochlorophyll b . Here we report an improved genome sequence of Blastochloris viridis DSM133, which is instrumental to the studies of photosynthesis, metabolic versatility, and genetic engineering of this microorganism.

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