scholarly journals Wide-field mosaics of the corneal subbasal nerve plexus in Parkinson’s disease using in vivo confocal microscopy

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Reza A. Badian ◽  
Stephan Allgeier ◽  
Fabio Scarpa ◽  
Mattias Andréasson ◽  
Andreas Bartschat ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Confocal Microscopy ◽  
Cellular Level ◽  
Nerve Plexus ◽  
Control Group ◽  
Wide Field ◽  
Image Size ◽  
Subbasal Nerve Plexus

AbstractIn vivo confocal microscopy (IVCM) is a non-invasive imaging technique facilitating real-time acquisition of images from the live cornea and its layers with high resolution (1–2 µm) and high magnification (600 to 800-fold). IVCM is extensively used to examine the cornea at a cellular level, including the subbasal nerve plexus (SBNP). IVCM of the cornea has thus gained intense interest for probing ophthalmic and systemic diseases affecting peripheral nerves. One of the main drawbacks, however, is the small field of view of IVCM, preventing an overview of SBNP architecture and necessitating subjective image sampling of small areas of the SBNP for analysis. Here, we provide a high-quality dataset of the corneal SBNP reconstructed by automated mosaicking, with an average mosaic image size corresponding to 48 individual IVCM fields of view. The mosaic dataset represents a group of 42 individuals with Parkinson’s disease (PD) with and without concurrent restless leg syndrome. Additionally, mosaics from a control group (n = 13) without PD are also provided, along with clinical data for all included participants.

An in vivo confocal microscopy study of corneal changes in pseudoexfoliation syndrome

2018 ◽  
Vol 29 (5) ◽  
pp. 555-560 ◽  
Author(s):  
Luca Terracciano ◽  
Michela Cennamo ◽  
Eleonora Favuzza ◽  
Litasova Julia ◽  
Orsola Caporossi ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Anterior Segment ◽  
Senile Cataract ◽  
Nerve Plexus ◽  
Pseudoexfoliation Syndrome ◽  
Control Group ◽  
In Vivo Confocal Microscopy ◽  
Corneal Sensitivity ◽  
Corneal Layer

Purpose: To evaluate, through the in vivo confocal microscopy, the pathological changes of each corneal layer in eyes affected by pseudoexfoliation syndrome. Methods: We studied 40 eyes of 40 patients with diagnosis of unilateral senile cataract associated with pseudoexfoliation syndrome and 40 eyes of 40 control subjects with senile cataract without pseudoexfoliation syndrome. All patients underwent a complete ophthalmic examination including best corrected visual acuity, slit-lamp examination, corneal sensitivity measurement using a Cochet-Bonnet nylon thread esthesiometer, and anterior segment optical coherence tomography (Visante OCT, Carl Zeiss Meditec AG, Germany); in vivo confocal microscopy of corneal sections (endothelium, stroma, sub-basal nerve plexus, and superficial and basal epithelium) was performed with the ConfoScan 4.0 (Nidek, Japan). Results: In pseudoexfoliation syndrome group, the mean corneal sensitivity was 44.1 ± 1.3 mm and in the control group was 55.6 ± 4.7 mm. The corneas of the eyes with pseudoexfoliation syndrome were significantly less sensitive than those of control group eyes (p < 0.001). Pseudoexfoliation syndrome eyes had a lower nerve density and less nerve beadings and a higher degree of tortuosity in sub-basal plexus compared to the control group. The cell density of epithelial and endothelial layers was significantly lower in pseudoexfoliation syndrome eyes than controls. In 80% of pseudoexfoliation syndrome eyes, we found activated keratocytes and inflammatory cells in the anterior stroma. Conclusion: Our study demonstrates the morpho-structural corneal alterations in eyes affected by pseudoexfoliation syndrome, using corneal in vivo confocal microscopy as a non-invasive and high-reproducible technique to evaluate pathophysiology of each corneal layer; the sub-basal nerve plexus alterations are correlated with the lower corneal sensitivity.

Image Reconstruction of the Subbasal Nerve Plexus with In Vivo Confocal Microscopy

2011 ◽  
Vol 52 (9) ◽  
pp. 5022 ◽  
Author(s):  
Stephan Allgeier ◽  
Andrey Zhivov ◽  
Franz Eberle ◽  
Bernd Koehler ◽  
Susanne Maier ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Confocal Microscopy ◽  
Nerve Plexus ◽  
In Vivo Confocal Microscopy ◽  
Subbasal Nerve Plexus

scholarly journals Assessment of Corneal subbasal nerve plexus using confocal in vivo microscopy in patients with pseudoexfoliation syndrome after phacoemulsification

2018 ◽  
Vol 11 (2) ◽  
pp. 13-18 ◽  
Author(s):  
Vitaly V. Potyomkin ◽  
Tatyana S. Varganova ◽  
Irina V. Terehova ◽  
Elena V. Ageeva
Keyword(s):  
Cataract Surgery ◽  
Main Group ◽  
Nerve Plexus ◽  
Pseudoexfoliation Syndrome ◽  
Control Group ◽  
In Vivo Microscopy ◽  
Before And After ◽  
The Impact ◽  
Subbasal Nerve Plexus

Phacoemulsification (PE) is the leading method of cataract surgery. Purpose. To assess the impact of PE on corneal subbasal nerve plexus in patients with pseudoexfoliation syndrome (PEX) using confocal in vivo microscopy. Methods. 42 patients (42 eyes) were enrolled in the study. The main group consisted of 24 patients (24 eyes) with PEX syndrome, and 18 patients (18 eyes) without it composed the control group. Confocal in vivo microscopy was performed before and after PHACO. Results. In patients with PEX after PE, an increase in number of nerve branches and pellet-like structures in them were noticed (p < 0,05).

scholarly journals An in vivo confocal microscopy study of corneal changes in patients with systemic sclerosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eszter Szalai ◽  
Gabriella Szucs ◽  
Szilvia Szamosi ◽  
Zsuzsa Aszalos ◽  
Ildiko Afra ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Confocal Microscopy ◽  
Ocular Surface ◽  
Healthy Subjects ◽  
Ocular Surface Disease ◽  
Nerve Plexus ◽  
In Vivo Confocal Microscopy ◽  
Software Analysis ◽  
Subbasal Nerve Plexus

AbstractTo investigate corneal microstructure of systemic sclerosis (SSc) patients using in vivo confocal microscopy (IVCM). 33 patients with SSc and 30 age-matched healthy subjects were recruited. All participants underwent comprehensive ophthalmic examination including IVCM (Heidelberg Retina Tomograph III, Heidelberg Engineering GmbH, Heidelberg, Germany) and ocular surface evaluation. Subbasal nerve plexus morphology was investigated using automated software analysis (ACCMetrics V3; University of Manchester, Manchester, UK). Keratocyte cell densities in the anterior stroma were significantly lower in patients with SSc compared to controls (P < 0.0001). In 7 SSc patients no keratocyte nuclei were identified in the anterior stroma and in most patients scattered hyperreflective punctate material were observed in the anterior stroma. Significantly lower subbasal nerve fiber parameters were found in patients with SSc compared to healthy subjects (P < 0.05). There were no significant correlations between the duration of SSc and any of the corneal cell density values. Tear break-up time values (4.82 ± 3.15 s) and Ocular Surface Disease Index scores (33.27 ± 30.11) were abnormal, Schirmer values (6.78 ± 5.82 mm) were borderline in SSc patients. In SSc, corneal morphological changes and accumulation of punctate material in the stroma was detected with confocal microscopy. Severe ocular surface disease was observed in SSc patients with significant impairment in subbasal nerve plexus morphology resembling peripheral neuropathy.

scholarly journals Parkinson’s disease with restless legs syndrome—an in vivo corneal confocal microscopy study

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mattias Andréasson ◽  
Neil Lagali ◽  
Reza A. Badian ◽  
Tor Paaske Utheim ◽  
Fabio Scarpa ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Confocal Microscopy ◽  
Restless Legs Syndrome ◽  
Cross Sectional ◽  
Restless Legs ◽  
Small Fiber ◽  
Corneal Confocal Microscopy ◽  
Large Fiber

AbstractSmall fiber neuropathy (SFN) has been suggested as a trigger of restless legs syndrome (RLS). An increased prevalence of peripheral neuropathy has been demonstrated in Parkinson’s disease (PD). We aimed to investigate, in a cross-sectional manner, whether SFN is overrepresented in PD patients with concurrent RLS relative to PD patients without RLS, using in vivo corneal confocal microscopy (IVCCM) and quantitative sensory testing (QST) as part of small fiber assessment. Study participants comprised of age- and sex-matched PD patients with (n = 21) and without RLS (n = 21), and controls (n = 13). Diagnosis of RLS was consolidated with the sensory suggested immobilization test. Assessments included nerve conduction studies (NCS), Utah Early Neuropathy Scale (UENS), QST, and IVCCM, with automated determination of corneal nerve fiber length (CNFL) and branch density (CNBD) from wide-area mosaics of the subbasal nerve plexus. Plasma neurofilament light (p-NfL) was determined as a measure of axonal degeneration. No significant differences were found between groups when comparing CNFL (p = 0.81), CNBD (p = 0.92), NCS (p = 0.82), and QST (minimum p = 0.54). UENS scores, however, differed significantly (p = 0.001), with post-hoc pairwise testing revealing higher scores in both PD groups relative to controls (p = 0.018 and p = 0.001). Analysis of all PD patients (n = 42) revealed a correlation between the duration of l-dopa therapy and CNBD (ρ = −0.36, p = 0.022), and p-NfL correlated with UENS (ρ = 0.35, p = 0.026) and NCS (ρ = −0.51, p = 0.001). Small and large fiber neuropathy do not appear to be associated with RLS in PD. Whether peripheral small and/or large fiber pathology associates with central neurodegeneration in PD merits further longitudinal studies.

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