Graphene Quantum Dots for Cell Proliferation, Nucleus Imaging, and Photoluminescent Sensing Applications

Abstract Background With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a disposable electrode with high-sensitivity and accuracy to evaluate its performances for several target viruses. Results Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-co-doped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of concentration from 1.45 × 102 to 1.45 × 105 DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml. Conclusion The engineered sensor electrode can retain the detection activity up to 5 weeks, to confirm its long-term stability, required for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing as a potential diagnostic tool to monitor WSSV in the aquaculture industry.

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Spotlighting graphene quantum dots and beyond: Synthesis, properties and sensing applications

Applied Materials Today ◽

10.1016/j.apmt.2017.09.002 ◽

2017 ◽

Vol 9 ◽

pp. 350-371 ◽

Cited By ~ 29

Author(s):

Manila Ozhukil Valappil ◽

Vijayamohanan K. Pillai ◽

Subbiah Alwarappan

Keyword(s):

Quantum Dots ◽

Graphene Quantum Dots ◽

Sensing Applications ◽

Synthesis Properties

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Carbon and graphene quantum dots: a review on syntheses, characterization, biological and sensing applications for neurotransmitter determination

RSC Advances ◽

10.1039/d0ra00799d ◽

2020 ◽

Vol 10 (26) ◽

pp. 15406-15429 ◽

Cited By ~ 22

Author(s):

Somayeh Tajik ◽

Zahra Dourandish ◽

Kaiqiang Zhang ◽

Hadi Beitollahi ◽

Quyet Van Le ◽

...

Keyword(s):

Quantum Dots ◽

Graphene Quantum Dots ◽

Sensing Applications

Carbon and graphene quantum dots for biological and sensing applications of neurotransmitters.

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Electrochemical Detection of White Spot Syndrome Virus With a Silicone Rubber Disposable Electrode Composed of Graphene Quantum Dots and Gold Nanoparticle-embedded Polyaniline Nanowires

10.21203/rs.3.rs-58911/v1 ◽

2020 ◽

Author(s):

Kenshin Takemura ◽

Jun Satoh ◽

Jirayu Boonyakida ◽

Sungjo Park ◽

Ankan Dutta Chowdhury ◽

...

Keyword(s):

Quantum Dots ◽

White Spot Syndrome Virus ◽

Graphene Quantum Dots ◽

High Sensitivity ◽

White Spot ◽

Sensing Matrix ◽

Disposable Electrode ◽

Sensing Applications ◽

Polyaniline Nanowires ◽

White Spot Syndrome

Abstract Background: With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a high-sensitivity and high-accuracy disposable electrode and evaluated it for several target viruses.Results: Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-codoped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of 1.45 × 102 to 1.45 × 10⁵ DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml.Conclusion: The engineered sensor electrode can retain the detection activity for up to 5 weeks, a vital long-term stability requirement for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing a potential diagnostic tool to monitor WSSV in the aquaculture industry.

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Waste derived approach towards wealthy fluorescent N-doped graphene quantum dots for cell imaging and H2O2 sensing applications

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2021.120453 ◽

2022 ◽

Vol 266 ◽

pp. 120453

Author(s):

Rahul V. Khose ◽

Prachi Bangde ◽

Mahesh P. Bondarde ◽

Pratik S. Dhumal ◽

Madhuri A. Bhakare ◽

...

Keyword(s):

Quantum Dots ◽

Cell Imaging ◽

Graphene Quantum Dots ◽

Sensing Applications ◽

Doped Graphene ◽

H2o2 Sensing

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Graphene quantum dots: recent progress in preparation and fluorescence sensing applications

RSC Advances ◽

10.1039/c6ra24349e ◽

2016 ◽

Vol 6 (112) ◽

pp. 110775-110788 ◽

Cited By ~ 68

Author(s):

Shenghai Zhou ◽

Hongbo Xu ◽

Wei Gan ◽

Qunhui Yuan

Keyword(s):

Quantum Dots ◽

Recent Progress ◽

Graphene Quantum Dots ◽

Fluorescence Sensing ◽

Sensing Applications

This paper reviews recent activities in the preparation and fluorescence sensing applications of graphene quantum dots.

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Hydroxyl edge-functionalized graphene quantum dots for gas-sensing applications

Diamond and Related Materials ◽

10.1016/j.diamond.2020.107790 ◽

2020 ◽

Vol 105 ◽

pp. 107790 ◽

Cited By ~ 6

Author(s):

Sarun Arunragsa ◽

Yotsarayuth Seekaew ◽

Weeraphat Pon-On ◽

Chatchawal Wongchoosuk

Keyword(s):

Quantum Dots ◽

Gas Sensing ◽

Graphene Quantum Dots ◽

Functionalized Graphene ◽

Sensing Applications

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Synthesizing Graphene Quantum Dots for Gas Sensing Applications

Procedia Engineering ◽

10.1016/j.proeng.2016.11.356 ◽

2016 ◽

Vol 168 ◽

pp. 1312-1316 ◽

Cited By ~ 16

Author(s):

D. Raeyani ◽

S. Shojaei ◽

S. Ahmadi Kandjani ◽

W. Wlodarski

Keyword(s):

Quantum Dots ◽

Gas Sensing ◽

Graphene Quantum Dots ◽

Sensing Applications

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Electron-Tunneling Modulation in Percolating Network of Graphene Quantum Dots: Fabrication, Phenomenological Understanding, and Humidity/Pressure Sensing Applications

Nano Letters ◽

10.1021/nl4003443 ◽

2013 ◽

Vol 13 (4) ◽

pp. 1757-1763 ◽

Cited By ~ 102

Author(s):

T. S. Sreeprasad ◽

Alfredo Alexander Rodriguez ◽

Jonathan Colston ◽

Augustus Graham ◽

Evgeniy Shishkin ◽

...

Keyword(s):

Quantum Dots ◽

Electron Tunneling ◽

Graphene Quantum Dots ◽

Pressure Sensing ◽

Sensing Applications ◽

Percolating Network

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Graphene Quantum Dots Electrochemistry and Development of Ultrasensitive Enzymatic Glucose Sensor

MRS Advances ◽

10.1557/adv.2018.324 ◽

2018 ◽

Vol 3 (15-16) ◽

pp. 831-847 ◽

Cited By ~ 4

Author(s):

Sanju Gupta ◽

Tyler Smith ◽

Alexander Banaszak ◽

John Boeckl

Keyword(s):

Quantum Dots ◽

Electron Transfer ◽

Cyclic Voltammetry ◽

Glucose Sensor ◽

Limit Of Detection ◽

Direct Electron Transfer ◽

Graphene Quantum Dots ◽

Optimal Size ◽

Sensing Applications ◽

Edge Site

AbstractGraphene quantum dots (GQDs) - zero-dimensional materials - are sheets of a few nanometers in lateral dimension and exhibit quantum confinement and edge site effects where sp2-bonded carbon nanocore surrounded with edged plane functional moieties is promising as advanced electroactive sensing platforms. In this work, GQDs are synthesized by solvothermal and hydrothermal techniques, with optimal size of 5 nm. Their potential in fundamental (direct electron transfer) and applied (enzymatic glucose biosensor) electrochemistry are demonstrated. Glucose oxidase (GOx) immobilized on glassy carbon (GC) electrodes modified with GQDs are investigated by means of cyclic voltammetry, differential pulse voltammetry, and amperometry. Well-defined quasi-reversible redox peaks observed under various electrochemical parameters helped to determine diffusion coefficient (D) and first-order electron transfer rate (kET). The cyclic voltammetry curves showed homogeneous ion transport for GQD with D ranging between 8.45 × 10−9 m2 s−1 and 3 × 10−8 m2 s−1 following GO < rGO < GQD < GQD (with FcMeOH as redox probe) < GOx/rGO < GOx/GO < HRP/GQDs < GOx/GQDs. The developed GOx-GQDs biosensor responds efficiently and linearly to the presence of glucose over concentrations ranging 10 μM and 3 mM with limit of detection 1.35 μM and sensitivity 0.00769 μA μM−1·cm−2 as compared with rGO (0.025 μA μM−1 cm−2, 4.16 μM) and GO (0.064 μA μM−1 cm−2, 4.82 μM) nanosheets. The high performance and stability of GQDs is attributed to sufficiently large surface-to-volume ratio, excellent biocompatibility, abundant hydrophilic edge site density, and partially hydrophobic planar sites that favors GOx adsorption on the electrode surface and versatile architectures to ensure rapid charge transfer and electron/ion conduction (<10 ms). We also carried out similar studies with other enzymatic protein biomolecules on electrode surfaces prepared from GQD precursors for electrochemical comparison, thus opening up potential sensing applications in medicine as well as bio-nanotechnology.

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