Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires

Abstract Background With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a disposable electrode with high-sensitivity and accuracy to evaluate its performances for several target viruses. Results Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-co-doped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of concentration from 1.45 × 102 to 1.45 × 105 DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml. Conclusion The engineered sensor electrode can retain the detection activity up to 5 weeks, to confirm its long-term stability, required for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing as a potential diagnostic tool to monitor WSSV in the aquaculture industry.

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Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires

10.21203/rs.3.rs-58911/v2 ◽

2020 ◽

Author(s):

Kenshin Takemura ◽

Jun Satoh ◽

Jirayu Boonyakida ◽

Sungjo Park ◽

Ankan Dutta Chowdhury ◽

...

Keyword(s):

Quantum Dots ◽

White Spot Syndrome Virus ◽

Graphene Quantum Dots ◽

High Sensitivity ◽

White Spot ◽

Sensing Matrix ◽

Disposable Electrode ◽

Sensing Applications ◽

Doped Graphene ◽

White Spot Syndrome

Abstract Background: With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a disposable electrode with high-sensitivity and accuracy to evaluate its performances for several target viruses.Results: Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-co-doped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of concentration from 1.45 × 102 to 1.45 × 10⁵ DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml.Conclusion: The engineered sensor electrode can retain the detection activity up to 5 weeks, to confirm its long-term stability, required for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing as a potential diagnostic tool to monitor WSSV in the aquaculture industry.

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Electrochemical Detection of White Spot Syndrome Virus With a Silicone Rubber Disposable Electrode Composed of Graphene Quantum Dots and Gold Nanoparticle-embedded Polyaniline Nanowires

10.21203/rs.3.rs-58911/v1 ◽

2020 ◽

Author(s):

Kenshin Takemura ◽

Jun Satoh ◽

Jirayu Boonyakida ◽

Sungjo Park ◽

Ankan Dutta Chowdhury ◽

...

Keyword(s):

Quantum Dots ◽

White Spot Syndrome Virus ◽

Graphene Quantum Dots ◽

High Sensitivity ◽

White Spot ◽

Sensing Matrix ◽

Disposable Electrode ◽

Sensing Applications ◽

Polyaniline Nanowires ◽

White Spot Syndrome

Abstract Background: With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a high-sensitivity and high-accuracy disposable electrode and evaluated it for several target viruses.Results: Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-codoped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of 1.45 × 102 to 1.45 × 10⁵ DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml.Conclusion: The engineered sensor electrode can retain the detection activity for up to 5 weeks, a vital long-term stability requirement for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing a potential diagnostic tool to monitor WSSV in the aquaculture industry.

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Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires

10.21203/rs.3.rs-58911/v3 ◽

2020 ◽

Author(s):

Kenshin Takemura ◽

Jun Satoh ◽

Jirayu Boonyakida ◽

Sungjo Park ◽

Ankan Dutta Chowdhury ◽

...

Keyword(s):

Quantum Dots ◽

White Spot Syndrome Virus ◽

Graphene Quantum Dots ◽

High Sensitivity ◽

White Spot ◽

Sensing Matrix ◽

Disposable Electrode ◽

Sensing Applications ◽

Polyaniline Nanowires ◽

White Spot Syndrome

Abstract Background: With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a high-sensitivity and high-accuracy disposable electrode and evaluated it for several target viruses. Results: Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-codoped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which wassuccessfully applied for the detection of WSSV over a wide linear range of 1.45 × 10 2 to 1.45 × 10⁵ DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml. Conclusion: The engineered sensor electrode can retain the detection activity for up to 5 weeks, a vital long-term stability requirement for disposable sensing applications. This is the first demonstration of the detection of WSSV by ananofabricated sensing electrode with high sensitivity, selectivity, and stability, providing a potential diagnostic tool to monitor WSSV in the aquaculture industry.

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Nanostructured carbon electrode modified with N-doped graphene quantum dots–chitosan nanocomposite: a sensitive electrochemical dopamine sensor

Royal Society Open Science ◽

10.1098/rsos.171199 ◽

2017 ◽

Vol 4 (11) ◽

pp. 171199 ◽

Cited By ~ 24

Author(s):

Sami Ben Aoun

Keyword(s):

Quantum Dots ◽

Hydrothermal Reaction ◽

Limit Of Detection ◽

Graphene Quantum Dots ◽

High Sensitivity ◽

Carbon Electrode ◽

Limit Of Quantification ◽

Real Sample Analysis ◽

Nitrogen Doped Graphene ◽

Doped Graphene

A highly selective and sensitive dopamine electrochemical sensor based on nitrogen-doped graphene quantum dots–chitosan nanocomposite-modified nanostructured screen printed carbon electrode is presented, for the first time. Graphene quantum dots were prepared via microwave-assisted hydrothermal reaction of glucose, and nitrogen doping was realized by introducing ammonia in the reaction mixture. Chitosan incorporation played a significant role towards the selectivity of the prepared sensor by hindering the ascorbic acid interference and enlarging the peak potential separation between dopamine and uric acid. The proposed sensor's performance was shown to be superior to several recently reported investigations. The as-prepared CS/N,GQDs@SPCE exhibited a high sensitivity (i.e. ca. 418 µA mM cm −2 ), a wide linear range i.e. (1–100 µM) and (100–200 µM) with excellent correlations (i.e. R 2 = 0.999 and R 2 = 1.000, respectively) and very low limit of detection (LOD = 0.145 µM) and limit of quantification (LOQ = 0.482 µM) based on S / N = 3 and 10, respectively. The applicability of the prepared sensor for real sample analysis was tested by the determination of dopamine in human urine in pH 7.0 PBS showing an approximately 100% recovery with RSD < 2% inferring both the practicability and reliability of CS/N,GQDs@SPCE. The proposed sensor is endowed with high reproducibility (i.e. RSD = ca. 3.61%), excellent repeatability (i.e. ca. 0.91% current change) and a long-term stability (i.e. ca. 94.5% retained activity).

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High sensitivity immunochromatographic strip test (ICP11 strip test) for white spot syndrome virus detection using monoclonal antibodies specific to ICP11 non-structural protein

Aquaculture ◽

10.1016/j.aquaculture.2016.12.004 ◽

2017 ◽

Vol 470 ◽

pp. 25-31 ◽

Cited By ~ 5

Author(s):

Pradit Wangman ◽

Ruthairat Siriwattanarat ◽

Siwaporn Longyant ◽

Chalinan Pengsuk ◽

Paisarn Sithigorngul ◽

...

Keyword(s):

Monoclonal Antibodies ◽

White Spot Syndrome Virus ◽

Virus Detection ◽

High Sensitivity ◽

White Spot ◽

Structural Protein ◽

Immunochromatographic Strip ◽

Immunochromatographic Strip Test ◽

White Spot Syndrome ◽

Strip Test

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Waste derived approach towards wealthy fluorescent N-doped graphene quantum dots for cell imaging and H2O2 sensing applications

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2021.120453 ◽

2022 ◽

Vol 266 ◽

pp. 120453

Author(s):

Rahul V. Khose ◽

Prachi Bangde ◽

Mahesh P. Bondarde ◽

Pratik S. Dhumal ◽

Madhuri A. Bhakare ◽

...

Keyword(s):

Quantum Dots ◽

Cell Imaging ◽

Graphene Quantum Dots ◽

Sensing Applications ◽

Doped Graphene ◽

H2o2 Sensing

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Recent advances in heteroatom-doped graphene quantum dots for sensing applications

RSC Advances ◽

10.1039/d1ra04248c ◽

2021 ◽

Vol 11 (41) ◽

pp. 25586-25615

Author(s):

Neeraj Sohal ◽

Banibrata Maity ◽

Soumen Basu

Keyword(s):

Quantum Dots ◽

Graphene Quantum Dots ◽

Sensing Applications ◽

Recent Advances ◽

Doped Graphene ◽

Fluorescent Nanomaterials ◽

Carbon Based

Graphene quantum dots (GQDs) are carbon-based fluorescent nanomaterials having various applications due to attractive properties.

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Zero-dimensional heterostructures: N-doped graphene dots/SnO2 for ultrasensitive and selective NO2 gas sensing at low temperatures

Journal of Materials Chemistry A ◽

10.1039/d0ta03037f ◽

2020 ◽

Vol 8 (23) ◽

pp. 11734-11742

Author(s):

Rahul Purbia ◽

Yeong Min Kwon ◽

Hong-Dae Kim ◽

Yun Sik Lee ◽

Heungjoo Shin ◽

...

Keyword(s):

Quantum Dots ◽

Gas Sensor ◽

Low Temperatures ◽

Gas Sensing ◽

Graphene Quantum Dots ◽

High Sensitivity ◽

Facile Synthesis ◽

Doped Graphene ◽

Excellent Selectivity

Facile synthesis of zero-dimensional heterostructures consisting of N-doped graphene quantum dots (N-GDs) and SnO2 nanoparticles is reported for the NO2 gas sensor with high sensitivity and excellent selectivity.

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