scholarly journals DISCOVER: A facile structure-based screening method for vinyl compound producing microbes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yuji Aso ◽  
Mei Sano ◽  
Hikari Kuroda ◽  
Hitomi Ohara ◽  
Hiroshi Ando ◽  
...  
Keyword(s):  
Heck Reaction ◽  
Aspergillus Terreus ◽  
Agar Medium ◽  
Screening Method ◽  
Coupling Reactions ◽  
Ene Reaction ◽  
Vinyl Compound ◽  
Novel Structure ◽  
Iodine Test ◽  
Microbial Screening

Abstract Here we report a novel structure-based microbial screening method for vinyl compound discovery, DISCOVER (direct screening method based on coupling reactions for vinyl compound producers). Through a two-step screening procedure based on selective coupling reactions of terminal alkenes, the thiol-ene reaction (1st step of screening) and Mizoroki-Heck reaction, followed by iodine test (2nd step of screening), microbes producing vinyl compounds like itaconic acid (IA) can be isolated from soil samples. In the 1st step of screening, soil sources are plated on agar medium supplemented with an antimicrobial agent, α-thioglycerol (TG), and a radical initiator, VA-044 (VA). In the 2nd step of screening, vinyl compounds produced in the cultures are labelled with iodobenzene via the Mizoroki-Heck reaction, followed by an iodine test, leading to the detection and characterisation of labelled products. We evaluated the validity of DISCOVER using IA and its producer Aspergillus terreus. Experimental data supported our hypothesis that IA reacts with TG in the medium via the thiol-ene reaction and consequently, A. terreus rapidly forms colonies on the agar medium because of the loss of the antimicrobial activity of TG. Using DISCOVER, high throughput and selective isolation of A. terreus strains producing IA was possible from soils.

scholarly journals Microbial Screening Based on the Mizoroki–Heck Reaction Permits Exploration of Hydroxyhexylitaconic-Acid-Producing Fungi in Soils

2020 ◽  
Vol 8 (5) ◽  
pp. 648 ◽  
Author(s):  
Mei Sano ◽  
Ryoki Yada ◽  
Yusuke Nomura ◽  
Takahiro Kusukawa ◽  
Hiroshi Ando ◽  
...  
Keyword(s):  
Cell Line ◽  
Heck Reaction ◽  
Screening Method ◽  
Cancer Cell Line ◽  
Diploid Cell ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela ◽  
Human Cervical Cancer Cell ◽  
Microbial Screening ◽  
Diploid Cell Line

Recently, we developed a unique microbial screening method based on the Mizoroki–Heck reaction for itaconic acid (IA)-producing fungi. This method revealed that 37 out of 240 fungal strains isolated from soils produce vinyl compounds, including IA. In this study, we further characterized these compounds in order to verify that the screening method permits the isolation of fungi that produce other vinyl compounds, excluding IA. HPLC analysis showed that 11 out of 37 isolated strains produced IA, similar to Aspergillus terreus S12-1. Surprisingly, the other 8 isolated strains produced two vinyl compounds with HPLC retention times different from that of IA. From these strains, the vinyl compounds of Aspergillus niger S17-5 were characterized. Mass spectrometric and NMR analyses showed that they were identical to 8-hydroxyhexylitaconic acid (8-HHIA) and 9-HHIA. This finding showed that 8-HHIA- and 9-HHIA-producing fungi, as well as IA-producing fungi, are ubiquitously found in soils. Neither 8-HHIA nor 9-HHIA showed antibacterial or anti-inflammatory activities. Interestingly, 8-HHIA and 9-HHIA showed cytotoxicity against the human cervical cancer cell line (HeLa) and human diploid cell line (MRC-5), and MRC-5 only, respectively, compared to IA at the same concentration. This study indicates that the screening method could easily discover fungi producing 8-HHIA and 9-HHIA in soils.

Sandwich Immunoassay as a High-Throughput Screening Method for Cross-Coupling Reactions

2005 ◽  
Vol 117 (42) ◽  
pp. 7023-7026 ◽  
Author(s):  
Paola Vicennati ◽  
Nicolas Bensel ◽  
Alain Wagner ◽  
Christophe Créminon ◽  
Frédéric Taran
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Method ◽  
Cross Coupling ◽  
Sandwich Immunoassay ◽  
Coupling Reactions ◽  
Cross Coupling Reactions

Novel glycosyl pyridyl-triazole@palladium nanoparticles: efficient and recoverable catalysts for C–C cross-coupling reactions

2015 ◽  
Vol 5 (4) ◽  
pp. 2065-2071 ◽  
Author(s):  
Hongyun Shen ◽  
Chao Shen ◽  
Chao Chen ◽  
Anming Wang ◽  
Pengfei Zhang
Keyword(s):  
Heck Reaction ◽  
Palladium Nanoparticles ◽  
Cross Coupling ◽  
Sonogashira Reaction ◽  
Coupling Reactions ◽  
Cross Coupling Reactions ◽  
Recoverable Catalysts

A series of glycosyl pyridyl-triazole@palladium nanoparticles have been prepared and applied in efficient C–C coupling such as Suzuki–Miyaura coupling, Heck reaction and Sonogashira reaction.

Sandwich Immunoassay as a High-Throughput Screening Method for Cross-Coupling Reactions

2005 ◽  
Vol 44 (42) ◽  
pp. 6863-6866 ◽  
Author(s):  
Paola Vicennati ◽  
Nicolas Bensel ◽  
Alain Wagner ◽  
Christophe Créminon ◽  
Frédéric Taran
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Method ◽  
Cross Coupling ◽  
Sandwich Immunoassay ◽  
Coupling Reactions ◽  
Cross Coupling Reactions

scholarly journals A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils

Heliyon ◽  
2019 ◽  
Vol 5 (7) ◽  
pp. e02048 ◽  
Author(s):  
Mei Sano ◽  
Hikari Kuroda ◽  
Hitomi Ohara ◽  
Hiroshi Ando ◽  
Keiji Matsumoto ◽  
...  
Keyword(s):  
High Throughput ◽  
Itaconic Acid ◽  
Heck Reaction ◽  
High Throughput Screening ◽  
Screening Method

scholarly journals Echinocandins and their Activity against Aspergillus terreus Species Complex: A Novel Agar Screening Method

2021 ◽  
Author(s):  
Nina Lackner ◽  
Roya Vahedi-Shahandashti ◽  
Sonja Jähnig ◽  
Lea Schönherr ◽  
Cornelia Lass-Flörl
Keyword(s):  
Species Complex ◽  
Aspergillus Terreus ◽  
Susceptibility Testing ◽  
Screening Method ◽  
The Novel ◽  
Wild Type ◽  
Type Strains

We evaluated the newly proposed agar-screening method for echinocandin susceptibility testing of 144 Aspergillus section Terrei isolates in comparison with Etest®. Both methods defined the isolates to be wild type strains for anidulafungin and micafungin, with Etest® minimal effective concentrations (MECs) being ≤0.004 mg/L. For caspofungin, the novel agar-screening method identified 37 isolates to be caspofungin non-wild type based on their fluffy colony appearance on caspofungin agar. Etest® MECs for caspofungin for these isolates scattered widely from 0.002 to 0.750 mg/L, showing only partial accordance between the two methods.

scholarly journals Rapid Dye Decolorization Method for Screening Potential Wood Preservatives

2000 ◽  
Vol 66 (12) ◽  
pp. 5457-5459 ◽  
Author(s):  
Olga Borokhov ◽  
Stephen Rothenburger
Keyword(s):  
Direct Observation ◽  
Agar Medium ◽  
Screening Method ◽  
Wood Decay ◽  
Dye Decolorization ◽  
Wood Preservatives ◽  
Inhibitory Effects ◽  
Decay Fungi ◽  
Wood Decay Fungi ◽  
Oxidative Agents

ABSTRACT We developed a new screening method for potential wood preservatives based on decolorization of the dye Remazol Brilliant Blue R by extracellular oxidative agents produced by wood decay fungi. Oxidative biodegradation of lignin yielded decolorized zones around and under fungal cultures on a dyed agar medium. Inhibitory effects were detected by direct observation and measurement of the decolorized zones.

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