scholarly journals Effects of different combined regimens of cisplatin, metformin, and quercetin on nasopharyngeal carcinoma cells and subcutaneous xenografts

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhongwei Chen ◽  
Zhen Zeng ◽  
Shanshan Zhu ◽  
Ying Zeng ◽  
Qihuang Lin ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Drug Combination ◽  
Drug Application ◽  
Inhibitory Effects ◽  
Single Drug ◽  
Combined Application ◽  
Subcutaneous Xenograft ◽  
Recurrent Nasopharyngeal Carcinoma ◽  
Combination Regimens

AbstractCisplatin, metformin, and quercetin are all reliable anticancer drugs. However, it is unclear how effective their different combination regimens are on the growth of nasopharyngeal carcinoma cell line Sune-1 and subcutaneous xenograft in nude mice. This study evaluated the effects of single-drug, two-drug, and three-drug simultaneous or sequential combined application of these drugs on the growth of Sune-1 cells and subcutaneous xenograft tumors in nude mice. The results showed that the different combination regimens of cisplatin, metformin and quercetin all had significant inhibitory effects on the proliferation of Sune-1 cells and the growth of subcutaneous xenografts in nude mice (P < 0.01), and the inhibition rate of the three drugs simultaneous combined application was significant Higher than the two-drug combination or single-drug application (P < 0.05), the contribution level of each drug in the three-drug combination application from high to low were cisplatin > metformin > quercetin. In summary, our results indicate that the simultaneous combination of cisplatin, metformin, and quercetin may synergistically inhibit the growth of Sune-1 cells and subcutaneous xenografts in nude mice through their different anticancer mechanisms, which may be clinically refractory and provide reference for chemotherapy of patients with recurrent nasopharyngeal carcinoma.

Inhibitory effects of myocardial cells culture medium on growth of human nasopharyngeal carcinoma and its mechanism in nude mice

2010 ◽  
Vol 34 (8) ◽  
pp. S53-S53
Author(s):  
Jing‑Bo Wu ◽  
Yu Zheng ◽  
Ling‑lin Yang ◽  
Qing‑Lian Wen ◽  
Zhou Su ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Culture Medium ◽  
Myocardial Cells ◽  
Inhibitory Effects ◽  
Human Nasopharyngeal Carcinoma

FOXD3 inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma through regulation of the PI3K–Akt pathway

2020 ◽  
Vol 98 (6) ◽  
pp. 653-660 ◽  
Author(s):  
Xiaoxing Xie ◽  
Gaoyun Xiong ◽  
Wenjun Chen ◽  
Hongdan Fu ◽  
Mingqian Li ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Nasopharyngeal Carcinoma ◽  
Cell Apoptosis ◽  
Inhibitory Effect ◽  
Akt Pathway ◽  
Migration And Invasion ◽  
Inhibitory Effects ◽  
Protein Levels ◽  
Flow Cytometric ◽  
Phosphoinositide 3 Kinase

FOXD3 has been found previously to positively regulate miR-26b, a tumor inhibitor of nasopharyngeal carcinoma (NPC). However, FOXD3’s precise function and associated mechanism of action in NPC have not yet been investigated. In this study, the expression of FOXD3 mRNA and protein was evaluated using RT-qPCR, western blotting, and immunohistochemistry. Protein levels involved in the phosphoinositide 3-kinase – protein kinase B (PI3K–Akt) pathway were assessed by western blot, and cell proliferation was determined by MTT and colony forming assays. Additionally, cell apoptosis was assessed by flow cytometric assay. Finally, the migration and invasion capabilities of the NPC cells were determined using wound healing and Transwell assays. We found that FOXD3 levels were relatively low in NPC tissue and cells, while an increase caused the inhibition of the PI3K–Akt pathway. Functional experiments found that overexpression of FOXD3 suppressed cell proliferation, migration, and invasion and enhanced cell apoptosis in NPC C6661 cells. IGF-1, an activator of the PI3K–Akt pathway, reversed the inhibitory effect of FOXD3. Furthermore, we found upregulation of the PI3K–Akt pathway and upregulation of the inhibitory effects of FOXD3 on C6661 cellular activities. In conclusion, FOXD3 negatively affected the PI3K–Akt pathway to restrain the processes involved in C6661 cell pathology. These findings further exposed the function and downstream axis of FOXD3 in NPC and displayed a promising new target for NPC therapy.

Transpalatal endoscopic resection of residual nasopharyngeal carcinoma after sequential chemoradiotherapy

2004 ◽  
Vol 118 (12) ◽  
pp. 951-954 ◽  
Author(s):  
Jong-Lyel Roh
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Endoscopic Resection ◽  
Salvage Surgery ◽  
Limited Extent ◽  
Operative Morbidity ◽  
First Report ◽  
Recurrent Nasopharyngeal Carcinoma ◽  
Type Of Surgery

Residual or recurrent nasopharyngeal carcinoma (NPC) is usually managed by re-irradiation or salvage surgery. This case presented a residual NPC with limited extent and an accessible location in the nasopharynx after completion of sequential chemoradiotherapy. The lesion was completely resected via the transpalatal route under endoscopic visualization with no significant post-operative morbidity. This is the first report of NPC successfully treated by this type of surgery.

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