scholarly journals Molecular diversity and evolutionary trends of cysteine-rich peptides from the venom glands of Chinese spider Heteropoda venatoria

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jie Luo ◽  
Yiying Ding ◽  
Zhihao Peng ◽  
Kezhi Chen ◽  
Xuewen Zhang ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Molecular Diversity ◽  
Insect Pests ◽  
Phylogenetic Analyses ◽  
Spider Venom ◽  
Evolutionary Trends ◽  
The Family ◽  
Venom Glands ◽  
Venom Evolution ◽  
Expressed Sequence

AbstractHeteropoda venatoria in the family Sparassidae is highly valued in pantropical countries because the species feed on domestic insect pests. Unlike most other species of Araneomorphae, H. venatoria uses the great speed and strong chelicerae (mouthparts) with toxin glands to capture the insects instead of its web. Therefore, H. venatoria provides unique opportunities for venom evolution research. The venom of H. venatoria was explored by matrix-assisted laser desorption/ionization tandem time-of-flight and analyzing expressed sequence tags. The 154 sequences coding cysteine-rich peptides (CRPs) revealed 24 families based on the phylogenetic analyses of precursors and cysteine frameworks in the putative mature regions. Intriguingly, four kinds of motifs are first described in spider venom. Furthermore, combining the diverse CRPs of H. venatoria with previous spider venom peptidomics data, the structures of precursors and the patterns of cysteine frameworks were analyzed. This work revealed the dynamic evolutionary trends of venom CRPs in H. venatoria: the precursor has evolved an extended mature peptide with more cysteines, and a diminished or even vanished propeptides between the signal and mature peptides; and the CRPs evolved by multiple duplications of an ancestral ICK gene as well as recruitments of non-toxin genes.

Molecular diversification based on analysis of expressed sequence tags from the venom glands of the Chinese bird spider Ornithoctonus huwena,

Toxicon ◽  
2008 ◽  
Vol 51 (8) ◽  
pp. 1479-1489 ◽  
Author(s):  
Liping Jiang ◽  
Li Peng ◽  
Jinjun Chen ◽  
Yongqun Zhang ◽  
Xia Xiong ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Venom Glands ◽  
Expressed Sequence ◽  
Ornithoctonus Huwena

scholarly journals Expressed sequence tags in venomous tissue of Scorpaena plumieri (Scorpaeniformes: Scorpaenidae)

2014 ◽  
Vol 12 (4) ◽  
pp. 871-878 ◽  
Author(s):  
Fábio L. S. Costa ◽  
Maria E. De Lima ◽  
Adriano C. Pimenta ◽  
Suely G. Figueiredo ◽  
Evanguedes Kalapothakis ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Venom Gland ◽  
Regulatory Proteins ◽  
Structural Proteins ◽  
Eastern Coast ◽  
Hypothetical Proteins ◽  
Respiratory Effects ◽  
The Family ◽  
Systemic Symptoms ◽  
Expressed Sequence

Species of the family Scorpaenidae are responsible for accidents and sporadic casualties by the shore they inhabit. The species Scorpaena plumierifrom this family populate the Northeastern and Eastern coast of Brazil causing human envenomation characterized by local and systemic symptoms. In experimental animals the venom induces cardiotoxic, hypotensive, and airway respiratory effects. As first step to identify the venom components we isolated gland mRNA to produce a cDNA library from the fish gland. This report describes the partial sequencing of 356 gland transcripts from S. plumieri. BLAST analysis of transcripts showed that 30% were unknown sequences, 17% hypothetical proteins, 17% related to metabolic enzymes, 14% belonged to signal transducing functions and the remaining groups (7-8%) composed by gene related with expressing proteins, regulatory proteins and structural proteins. A considerable number of these EST were not found in available databases suggesting the existence of new proteins and/or functions yet to be discovered. By screening the library with antibodies against a lectin fraction from S. plumierivenom we identified several clones whose DNA sequence showed similarities with lectins found in fish. In silicoanalysis of these clones confirm the identity of these molecules in the venom gland of S. plumieri.

Molecular diversity and evolutionary trends of cysteine-rich peptides from the venom glands of Chinese spider Heteropoda venatoria

2020 ◽  
Author(s):  
Yiying Ding ◽  
Kezhi Chen ◽  
Xuewen Zhang ◽  
Tiaoyi Xiao ◽  
Jinjun Chen
Keyword(s):  
Insect Pests ◽  
Venom Gland ◽  
Evolutionary Analysis ◽  
Evolutionary Trends ◽  
Holistic View ◽  
Carl Linnaeus ◽  
Peptide Precursor ◽  
Venom Glands ◽  
Peptide Toxin ◽  
Peptide Toxins

Abstract Background: The huntsman spider ( Heteropoda venatoria Carl Linnaeus, 1767) in family Sparassidae, is highly valued in tropical and subtropical countries because the species capture and feed on cockroaches and other domestic insect pests. Unlike most other species of Araneomorphae, the huntsman spiders do not use webs to capture prey. Their great speed and strong chelicerae (mouthparts) with toxin glands are used to capture the insects.Results: We identified 154 novel putative cysteine-rich peptide toxin precursors by analyzing expressed sequence tags of the spider H. venatoria venom gland. The sequences of cysteine-rich peptide precursor revealed 24 families based on the phylogenetics analyses of signal peptide and cysteine framework in mature region, including 8 families of classic Inhibitory cystine knot toxins, 2 families of novel 6-cys motifs, 13 families of long cysteine-rich peptides with 8, 10 and 12-cys, and one family of 2-cys peptides. Intriguingly, four kinds of motifs are first described in spider venom. Furthermore, combining the diverse cysteine-rich peptide sequences of H. venatoria with the sequences from represent spiders explored previously, the dynamic evolutionary trends of venom cysteine-rich peptides were investigated based on the analysis of the structures of precursors and the patterns of cysteine scaffolds in the phylogenetic framework.Conclusion: H. venatoria is an appropriate intermediate species for the evolutionary analysis of spider peptide toxins from Mygalomorphae to Araneomorphae with a holistic view. This work revealed the dynamic evolutionary trends of venom cysteine-rich peptides of spider: the mature peptides have been developed longer with more cysteines; and the propeptides between the signal and mature peptides have been diminished and even vanished. With respect to potential insecticidal applications, the work provides promising new templates and gene clones for further exploration.

Transcriptome analysis of expressed sequence tags from the venom glands of the fish Thalassophryne nattereri

Biochimie ◽  
2006 ◽  
Vol 88 (6) ◽  
pp. 693-699 ◽  
Author(s):  
G.S. Magalhães ◽  
I.L.M. Junqueira-de-Azevedo ◽  
M. Lopes-Ferreira ◽  
D.M. Lorenzini ◽  
P.L. Ho ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Expressed Sequence Tags ◽  
Venom Glands ◽  
Expressed Sequence

Emergence of New Potential Insect Pests of Date Palm: Could Climate Change Be the Reason?

2019 ◽  
Vol 30 (6) ◽  
pp. 242-245
Author(s):  
Hamadttu A. F. El-Shafie
Keyword(s):  
Climate Change ◽  
Date Palm ◽  
Insect Pests ◽  
Anthropogenic Activities ◽  
Economic Damage ◽  
South American ◽  
Palm Fruit ◽  
The Family ◽  
American Palm ◽  
Sorghum Chafer

Four insect species were reported as new potential pests of date palm in recent years. They are sorghum chafer (Pachnoda interrupta), the rose chafer (Potosia opaca), the sericine chafer beetle (Maladera insanablis), and the South American palm borer (Pysandisia archon). The first three species belong to the order Coleoptera and the family Scarabaeidae, while the fourth species is a lepidopteran of the family Castniidae. The injury as well as the economic damage caused by the four species on date palm need to be quantified. Due to climate change and anthropogenic activities, the date palm pest complex is expected to change in the future. To the author's knowledge, this article provides the first report of sorghum chafer as a pest damaging date palm fruit.

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