scholarly journals Investigating BB0405 as a novel Borrelia afzelii vaccination candidate in Lyme borreliosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. J. Klouwens ◽  
J. J. Trentelman ◽  
J. I. Ersoz ◽  
F. Nieves Marques Porto ◽  
R. Sima ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Dna Vaccine ◽  
Lyme Borreliosis ◽  
Ixodes Ricinus ◽  
Dna Vaccination ◽  
Sensu Stricto ◽  
Antibody Responses ◽  
Mammalian Host ◽  
Control Groups ◽  
Borrelia Afzelii

AbstractBB0405 is a surface exposed Borrelia burgdorferi protein and its vaccination protected mice against B. burgdorferi infection. As BB0405 is highly conserved across different B. burgdorferi sensu lato species, we investigated whether vaccination with recombinant BB0405 or through intradermal bb0405 DNA tattoo vaccination could provide protection against different Borrelia species, specifically against Borrelia afzelii, the predominant B. burgdorferi sensu lato genospecies causing Lyme borreliosis across Eurasia. We immunized C3H/HeN mice with recombinant BB0405 or with a codon-optimized bb0405 DNA vaccine using the pVAC plasmid and immunized corresponding control groups mice with only adjuvant or empty vectors. We subsequently subjected these immunized mice to a tick challenge with B. afzelii CB43-infected Ixodes ricinus nymphs. Upon vaccination, recombinant BB0405 induced a high total IgG response, but bb0405 DNA vaccination did not elicit antibody responses. Both vaccine formulations did not provide protection against Borrelia afzelii strain CB43 after tick challenge. In an attempt to understand the lack of protection of the recombinant vaccine, we determined expression of BB0405 and showed that B. afzelii CB43 spirochetes significantly and drastically downregulate the expression of BB0405 protein at 37 °C compared to 33 °C, where as in B. burgdorferi B31 spirochetes expression levels remain unaltered. Vaccination with recombinant BB0405 was previously shown to protect against B. burgdorferi sensu stricto. Here we show that vaccination with either recombinant BB0405 (or non-immunogenic bb0405 DNA), despite being highly conserved among B. burgdorferi sl genospecies, does not provide cross-protection against B. afzelii, mostly likely due to downregulation of this protein in B. afzelii in the mammalian host.

Association of Borrelia afzelii with rodents in Europe

Parasitology ◽  
2003 ◽  
Vol 126 (1) ◽  
pp. 11-20 ◽  
Author(s):  
K. HANINCOVÁ ◽  
S. M. SCHÄFER ◽  
S. ETTI ◽  
H.-S. SEWELL ◽  
V. TARAGELOVÁ ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Borreliosis ◽  
Tick Infestation ◽  
Zoonotic Transmission ◽  
Host Association ◽  
Borrelia Afzelii ◽  
Hard Ticks ◽  
Bank Voles ◽  
Endemic Focus ◽  
Questing Ticks

Borrelia burgdorferi sensu lato (s.l.) is maintained in nature by complex zoonotic transmission cycles, involving a large variety of vertebrates as hosts and hard ticks of the genus Ixodes as vectors. Recent studies suggest that the genospecies of B. burgdorferi s.l. and sometimes their subtypes are propagated by different spectra of hosts, mainly birds and rodents. In order to test the concept of host-association, we analysed the relationships between Borrelia genospecies, rodent hosts and I. ricinus ticks in an endemic focus of Lyme borreliosis in western Slovakia. Rodents and questing ticks were collected at a forested lowland locality near Bratislava. Tick infestation levels on rodents were determined, and spirochaete infections in ticks and in ear punch biopsies were analysed by PCR followed by genotyping. Mice were more heavily infested with ticks than bank voles, and a higher proportion of mice was infected with spirochaetes than voles. However, the infectivity of voles was much higher than that of mice. The vast majority of infections detected in the skin and in ticks feeding on the rodents represented B. afzelii. In contrast, more than half of all infections in questing ticks collected in the same region of Slovakia were identified as B. valaisiana and B. garinii. In conclusion, whilst the study reveals that mice and voles play different quantitative roles in the ecology of Lyme borreliosis, it demonstrates that B. afzelii is specifically maintained by European rodents, validating the concept of host-association of B. burgdorferi s.l.

scholarly journals DNA Microarray Assessment of Putative Borrelia burgdorferi Lipoprotein Genes

2002 ◽  
Vol 70 (6) ◽  
pp. 3300-3303 ◽  
Author(s):  
Fang Ting Liang ◽  
F. Kenneth Nelson ◽  
Erol Fikrig
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Dna Microarray ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Genes Encoding ◽  
The Individual

ABSTRACT A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.

Infestation of sand lizards (Lacerta agilis) resident in the Northeastern Poland by Ixodes ricinus (L.) ticks and their infection with Borrelia burgdorferi sensu lato

2007 ◽  
Vol 52 (2) ◽  
Author(s):  
Alicja Gryczyńska-Siemiątkowska ◽  
Alicja Siedlecka ◽  
Joanna Stańczak ◽  
Miłosława Barkowska
Keyword(s):  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Tick Infestation ◽  
Sensu Stricto ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Passerine Birds ◽  
Northeastern Poland ◽  
Lacerta Agilis ◽  
Etiological Agents ◽  
Age And Sex

AbstractSand lizards (Lacerta agilis) were trapped and examined for ticks from May to September in 2002 and 2003 in Northeastern Poland. A total of 233 Ixodes ricinus (L.) ticks (76 larvae and 157 nymphs) was found on 31 of 235 captured lizards (13.2%). The tick infestation is relatively low compared to that of mammals and passerine birds from the same area (Siński et al. 2006, Gryczyńska et al. 2002). Tick infestation depended on the month of capture, being the highest in spring. In autumn no ticks were recorded on any of the captured lizards. The oldest lizards carried the highest number of ticks but no differences related to sex of the host were found. All the collected ticks were analysed by PCR for the presence of Borrelia burgdorferi sensu lato, the etiological agents of Lyme disease. Spirochetes were detected in 11 out of 233 (4.7%) ticks tested. Genetic analysis confirmed that the spirochetes are members of the Borrelia afzelii, B. garinii and B. burgdorferi sensu stricto genospecies. Mixed infection were not detected. The prevalence of infection was analysed in relation to months of the capture, age and sex of the lizards, but differences were not statistically significant. The obtained results suggest that lizards are probably not B. burgdorferi reservoirs, but further studies are required to confirm this.

scholarly journals Decorin Binding by DbpA and B of Borrelia garinii, Borrelia afzelii, and Borrelia burgdorferi Sensu Stricto

2011 ◽  
Vol 204 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Jemiina Salo ◽  
Vuokko Loimaranta ◽  
Pekka Lahdenne ◽  
Matti K. Viljanen ◽  
Jukka Hytönen
Keyword(s):  
Borrelia Burgdorferi ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Borrelia Burgdorferi Sensu Stricto

scholarly journals Antibodies to Borrelia burgdorferi OspA, OspC, OspF, and C6 Antigens as Markers for Early and Late Infection in Dogs

2012 ◽  
Vol 19 (4) ◽  
pp. 527-535 ◽  
Author(s):  
Bettina Wagner ◽  
Heather Freer ◽  
Alicia Rollins ◽  
David Garcia-Tapia ◽  
Hollis N. Erb ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
The United States ◽  
Sensu Stricto ◽  
Antibody Responses ◽  
Surface Proteins ◽  
Early Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Content Type ◽  
Antibody Levels

ABSTRACTLyme disease in the United States is caused byBorrelia burgdorferisensu stricto, which is transmitted to mammals by infected ticks.Borreliaspirochetes differentially express immunogenic outer surface proteins (Osp). Our aim was to evaluate antibody responses to Osp antigens to aid the diagnosis of early infection and the management of Lyme disease. We analyzed antibody responses during the first 3 months after the experimental infection of dogs using a novel multiplex assay. Results were compared to those obtained with two commercial assays detecting C6 antigen. Multiplex analysis identified antibodies to OspC and C6 as early as 3 weeks postinfection (p.i.) and those to OspF by 5 weeks p.i. Antibodies to C6 and OspF increased throughout the study, while antibodies to OspC peaked between 7 and 11 weeks p.i. and declined thereafter. A short-term antibody response to OspA was observed in 3/8 experimentally infected dogs on day 21 p.i. Quant C6 enzyme-linked immunosorbent assay (ELISA) results matched multiplex results during the first 7 weeks p.i.; however, antibody levels subsequently declined by up to 29%. Immune responses then were analyzed in sera from 125 client-owned dogs and revealed high agreement between antibodies to OspF and C6 as robust markers for infection. Results from canine patient sera supported that OspC is an early infection marker and antibodies to OspC decline over time. The onset and decline of antibody responses toB. burgdorferiOsp antigens and C6 reflect their differential expression during infection. They provide valuable tools to determine the stage of infection, treatment outcomes, and vaccination status in dogs.

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