scholarly journals Trichovariability in rhizosphere soil samples and their biocontrol potential against downy mildew pathogen in pearl millet

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Boregowda Nandini ◽  
Hariprasad Puttaswamy ◽  
Ramesh Kumar Saini ◽  
Harischandra Sripathy Prakash ◽  
Nagaraja Geetha
Keyword(s):  
Genetic Diversity ◽  
Distribution Pattern ◽  
Downy Mildew ◽  
Pearl Millet ◽  
Systemic Resistance ◽  
Diversity Profile ◽  
Biocontrol Potential ◽  
Agroclimatic Zones ◽  
Rapd And Issr ◽  
Direct Suppression

AbstractThe present work is aimed to examine the genetic variability and the distribution pattern of beneficial Trichoderma spp. isolated from rhizosphere samples and their mode of action in improving the plant health. A total of 131 suspected fungi were isolated from the rhizospheric soil and 91 isolates were confirmed as Trichoderma spp. T. asperellum and T. harzianum were found high in the frequency of occurrence. Genetic diversity analysis using RAPD and ISSR revealed the diverse distribution pattern of Trichoderma spp. indicating their capability to adapt to broad agroclimatic conditions. Analysis of genetic diversity using molecular markers revealed intra-species diversity of isolated Trichoderma spp. The frequency of pearl millet (PM) root colonization by Trichoderma spp. was found to be 100%. However, they showed varied results for indole acetic acid, siderophore, phosphate solubilization, β-1,3-glucanase, chitinase, cellulase, lipase, and protease activity. Downy mildew disease protection studies revealed a strong involvement of Trichoderma spp. in direct suppression of the pathogen (mean 37.41) in the rhizosphere followed by inducing systemic resistance. Our findings highlights the probable distribution and diversity profile of Trichoderma spp. as well as narrate the possible utilization of Trichoderma spp. as microbial fungicides in PM cultivation across different agroclimatic zones of India.

scholarly journals Biocontrol potential of three antagonists against downy mildew of pearl millet caused by Sclerospora graminicol (Sacc.) Schoret

2020 ◽  
Vol 34 (3) ◽  
pp. 180-184
Author(s):  
K K. SAINI ◽  
A C. MATHUR ◽  
R S. SHARMA ◽  
VIRENDRA KUMAR ◽  
R K. BAGRI ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Biocontrol Potential

scholarly journals Elicitation of Novel Trichogenic-Lipid Nanoemulsion Signaling Resistance Against Pearl Millet Downy Mildew Disease

Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 25 ◽  
Author(s):  
Boregowda Nandini ◽  
Hariprasad Puttaswamy ◽  
Harischandra Sripathy Prakash ◽  
Shivakanthkumar Adhikari ◽  
Sudisha Jogaiah ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Membrane Lipids ◽  
Growth Parameters ◽  
Droplet Diameter ◽  
Lipid Fraction ◽  
Systemic Resistance ◽  
Ionic Surfactant ◽  
Trichoderma Spp ◽  
Lipid Nanoemulsion

Nanoemulsion was formulated from membrane lipids of Trichoderma spp. with the non-ionic surfactant Tween 80 by the ultrasonic emulsification method. Nanoemulsion with a droplet diameter of 5 to 51 nm was obtained. The possible effects of membrane lipid nanoemulsion on pearl millet (PM) seed growth parameters and elicitation of downy mildew (DM) disease resistance in PM was analyzed to develop an eco-friendly disease management strategy. Seed priming with nanoemulsion illustrates significant protection and elevated levels of early defense gene expression. Lipid profiling of Trichoderma spp. reveals the presence of oleic acid as a major fatty acid molecule. The prominent molecule in the purified lipid fraction of T. brevicompactum (UP-91) responsible for the elicitation of induction of systemic resistance in PM host against DM pathogen was predicted as (E)-N-(1, 3-dihydroxyoctadec-4-en-2yl) acetamide. The results suggest that protection offered by the novel nanoemulsion formulation is systemic in nature and durable and offers a newer sustainable approach to manage biotrophic oomycetous pathogen.

scholarly journals Total crude protein extract of Trichoderma spp. induces systemic resistance in pearl millet against the downy mildew pathogen

3 Biotech ◽  
2017 ◽  
Vol 7 (3) ◽  
Author(s):  
Boregowda Nandini ◽  
Puttaswamy Hariprasad ◽  
Harohalli Nanjegowda Shankara ◽  
Harischandra Sripathy Prakash ◽  
Nagaraja Geetha
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Crude Protein ◽  
Systemic Resistance ◽  
Protein Extract ◽  
Trichoderma Spp ◽  
Crude Protein Extract ◽  
Total Crude Protein

Nitric oxide is involved in chitosan-induced systemic resistance in pearl millet against downy mildew disease

2009 ◽  
Vol 65 (7) ◽  
pp. 737-743 ◽  
Author(s):  
Girigowda Manjunatha ◽  
Sathyanaraya Niranjan-Raj ◽  
Geetha N Prashanth ◽  
Shantharaj Deepak ◽  
Kestur N Amruthesh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Downy Mildew ◽  
Pearl Millet ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Downy Mildew Disease

scholarly journals Efficacy of seed defense proteins in biofortified pearl millet lines against blast and downy mildew

2021 ◽  
Vol 43 (3) ◽  
Author(s):  
Marri Swathi ◽  
Nimmala Naresh ◽  
Tirupaati Swaroopa Rani ◽  
Mahalingam Govindaraj ◽  
Rajan Sharma
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Defense Proteins

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

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