scholarly journals Microbial sulfate reduction by Desulfovibrio is an important source of hydrogen sulfide from a large swine finishing facility

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Olga V. Karnachuk ◽  
Igor I. Rusanov ◽  
Inna A. Panova ◽  
Mikhail A. Grigoriev ◽  
Viacheslav S. Zyusman ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Large Scale ◽  
Solid Phase ◽  
Agricultural Waste ◽  
Reduction Rate ◽  
Gene Profiling ◽  
Sulfate Reduction Rate ◽  
Rrna Gene ◽  
Microbial Sulfate Reduction ◽  
Manure Slurry

AbstractThere is still a lack of understanding of H2S formation in agricultural waste, which leads to poor odour prevention and control. Microbial sulfate reduction is a major process contributing to sulfide formation in natural and technogenic environments with high sulfate and low oxygen concentration. Agricultural waste can be considered a low-sulfate system with no obvious input of oxidised sulfur compounds. The purpose of this study was to characterise a microbial community participating in H2S production and estimate the microbial sulfate reduction rate (SRR) in manure slurry from a large-scale swine finishing facility in Western Siberia. In a series of manure slurry microcosms, we identified bacterial consortia by 16S rRNA gene profiling and metagenomic analysis and revealed that sulfate-reducing Desulfovibrio were key players responsible for H2S production. The SRR measured with radioactive sulfate in manure slurry was high and comprised 7.25 nmol S cm−3 day−1. Gypsum may be used as a solid-phase electron acceptor for sulfate reduction. Another plausible source of sulfate is a swine diet, which often contains supplements in the form of sulfates, including lysine sulfate. Low-sulfur diet, manure treatment with iron salts, and avoiding gypsum bedding are possible ways to mitigate H2S emissions from swine manure.

Interactions between filamentous sulfur bacteria, sulfate reducing bacteria and poly-P accumulating bacteria in anaerobic-oxic activated sludge from a municipal plant

1998 ◽  
Vol 37 (4-5) ◽  
pp. 599-603 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
Edja. Kofi. Bosque-Hamilton
Keyword(s):  
Activated Sludge ◽  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reduction Rate ◽  
Filamentous Bulking ◽  
Sulfate Reducing ◽  
Sulfur Bacteria ◽  
Sulfate Reduction Rates ◽  
Reducing Bacteria

The interactions between filamentous sulfur bacteria (FSB), sulfate reducing bacteria (SRB) and poly-P accumulating bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions were examined in batch experiments using return sludge (RAS) and settled sewage. Phosphate release and sulfate reduction occurred simultaneously under anaerobic conditions. SRB were more sensitive to temperature changes than PAB. SRB played an important role in the decomposition of propionate to acetate. When the sulfate reduction rates were high, there was a tendency for the maximum release of phosphate also to be high. This was explained by the fact that PAB utilized the acetate produced by SRB. Sulfur oxidizing bacteria were sensitive to temperature change. When the sulfate reduction rate was high, the sulfide oxidizing rate was also high and filamentous bulking occurred. The results showed that sulfate reduction was a cause of filamentous bulking due to Type 021N that could utilize reduced sulfur.

scholarly journals Diversity of Sulfur Isotope Fractionations by Sulfate-Reducing Prokaryotes

2001 ◽  
Vol 67 (2) ◽  
pp. 888-894 ◽  
Author(s):  
Jan Detmers ◽  
Volker Brüchert ◽  
Kirsten S. Habicht ◽  
Jan Kuever
Keyword(s):  
Sulfate Reduction ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Reduction Rate ◽  
Sulfate Reduction Rate ◽  
Dissimilatory Sulfate Reduction ◽  
Systematic Effect ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation

ABSTRACT Batch culture experiments were performed with 32 different sulfate-reducing prokaryotes to explore the diversity in sulfur isotope fractionation during dissimilatory sulfate reduction by pure cultures. The selected strains reflect the phylogenetic and physiologic diversity of presently known sulfate reducers and cover a broad range of natural marine and freshwater habitats. Experimental conditions were designed to achieve optimum growth conditions with respect to electron donors, salinity, temperature, and pH. Under these optimized conditions, experimental fractionation factors ranged from 2.0 to 42.0‰. Salinity, incubation temperature, pH, and phylogeny had no systematic effect on the sulfur isotope fractionation. There was no correlation between isotope fractionation and sulfate reduction rate. The type of dissimilatory bisulfite reductase also had no effect on fractionation. Sulfate reducers that oxidized the carbon source completely to CO2 showed greater fractionations than sulfate reducers that released acetate as the final product of carbon oxidation. Different metabolic pathways and variable regulation of sulfate transport across the cell membrane all potentially affect isotope fractionation. Previous models that explained fractionation only in terms of sulfate reduction rates appear to be oversimplified. The species-specific physiology of each sulfate reducer thus needs to be taken into account to understand the regulation of sulfur isotope fractionation during dissimilatory sulfate reduction.

scholarly journals Salinity Responses of Benthic Microbial Communities in a Solar Saltern (Eilat, Israel)

2004 ◽  
Vol 70 (3) ◽  
pp. 1608-1616 ◽  
Author(s):  
Ketil Bernt S�rensen ◽  
Donald E. Canfield ◽  
Aharon Oren
Keyword(s):  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sulfate Reduction Rate ◽  
Anoxygenic Phototrophs ◽  
Sulfate Reducing ◽  
Oxygen Budget ◽  
Reducing Bacteria ◽  
Increased Activity ◽  
Optimum Salinity

ABSTRACT The salinity responses of cyanobacteria, anoxygenic phototrophs, sulfate reducers, and methanogens from the laminated endoevaporitic community in the solar salterns of Eilat, Israel, were studied in situ with oxygen microelectrodes and in the laboratory in slurries. The optimum salinity for the sulfate reduction rate in sediment slurries was between 100 and 120‰, and sulfate reduction was strongly inhibited at an in situ salinity of 215‰. Nevertheless, sulfate reduction was an important respiratory process in the crust, and reoxidation of formed sulfide accounted for a major part of the oxygen budget. Methanogens were well adapted to the in situ salinity but contributed little to the anaerobic mineralization in the crust. In slurries with a salinity of 180‰ or less, methanogens were inhibited by increased activity of sulfate-reducing bacteria. Unicellular and filamentous cyanobacteria metabolized at near-optimum rates at the in situ salinity, whereas the optimum salinity for anoxygenic phototrophs was between 100 and 120‰.

Ecological interactions among denitrification, poly-P accumulation, sulfate reduction, and filamentous sulfur bacteria in activated sludge

1994 ◽  
Vol 30 (11) ◽  
pp. 201-210 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori
Keyword(s):  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sodium Molybdate ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reduction Rate ◽  
Filamentous Bulking ◽  
Sulfate Reducing ◽  
Sulfur Bacteria ◽  
P Accumulation ◽  
Reducing Bacteria

Effects of anoxic-oxic conditions on the growth of sulfate reduction, poly-P accumulation and filamentous sulfur bacteria were examined in the laboratory scale sequential batch reactors. In the anoxic-oxic conditions, denitrification bacteria are dominant. The growth of sulfate reducing bacteria and poly-P accumulating bacteria was suppressed. The number of sulfate reducing bacteria in the activated sludge was below 104 MPN/g MLSS, and the sulfate reduction rate was very low. Filamentous bulking was also suppressed. On the other hand, when nitrate was removed from the artificial wastewater, sulfate reducing bacteria could grow predominantly in the anaerobic conditions. The number of sulfate reducing bacteria was about 106∼107 MPN/g MLSS and the sulfate reduction rate increased (0.17 ∼ 0.21 g SO4/g MLSS·hr). Filamentous bacteria Type 021N increased over 103 cm/mg MLSS. Sodium molybdate was added to the artificial wastewater in order to prevent sulfate reduction. When the concentration of sodium molybdate increased to 980 mg/L, the number of sulfate reducing bacteria decreased to 103 ∼ 104 MPN/g MLSS and the sulfate reduction rate decreased. Filamentous bulking was completely suppressed in these conditions. These results show that sulfate reduction is a main trigger of the filamentous bulking due to Type 021N that can utilize reduced sulfur for an energy source.

Competition between methanogenesis and sulfidogenesis in anaerobic wastewater treatment

1998 ◽  
Vol 38 (8-9) ◽  
pp. 317-324 ◽  
Author(s):  
Gong-Ming Zhou ◽  
Herbert H. P. Fang
Keyword(s):  
Wastewater Treatment ◽  
Sulfate Reduction ◽  
Methane Production ◽  
Reduction Rate ◽  
Uasb Reactor ◽  
Sulfate Reduction Rate ◽  
Sulfate Concentration ◽  
Anaerobic Wastewater Treatment ◽  
Sulfate Reducing ◽  
Sulfate Removal

This study was conducted to investigate the methanogenic and sulfidogenic activities of biomass in a UASB reactor treating wastewater containing benzoate (680 mg l−1) and sulfate (increased from 1080 to 2680 mg l−1) at 37°C and 12 hours of hydraulic retention. Results showed that after 120 days of acclimation, sludge consistently removed 99.5% of benzoate regardless of increased sulfate concentrations. Sulfidogenesis gradually out-competed methanogenesis during the acclimation phase, as indicated by the increase of sulfate-reducing efficiency (up to 99%) accompanied by the decrease of methane production. Overall sulfate removal efficiency was limited after the reactor had reached its maximum sulfate reduction rate of 2.1 g S (l d−1). Further increasing sulfate concentration from 1080 mg l−1 to 2680 mg l−1 lowered the sulfate-reducing efficiency from 85% to 39%. Flow of available electrons toward sulfidogenesis increased with the decrease of benzoate concentration, and was only slightly affected by the sulfate concentration or the benzoate/SO42−-S ratio.

A UASB bioreactor using silage as a carbon source to reduce sulfate

2011 ◽  
Vol 11 (2) ◽  
pp. 229-237 ◽  
Author(s):  
Winton Li ◽  
Susan A. Baldwin
Keyword(s):  
Carbon Source ◽  
Sulfate Reduction ◽  
Low Cost ◽  
Reduction Rate ◽  
Anaerobic Sludge ◽  
Sulfate Reduction Rate ◽  
Sulfate Removal ◽  
Cost Treatment ◽  
One Year ◽  
Agricultural Byproduct

Low cost-treatment for sulfate removal is required in many areas where potable water is scarce. The biggest challenge in biological treatment is finding an abundant low or no-cost carbon source. This work demonstrated for the first time that leachate from the agricultural byproduct silage can be used in an upflow anaerobic sludge-bed bioreactor to reduce sulfate for on-farm water treatment. The reactor ran continuously for approximately one year with an average silage leachate feed COD concentration of 4,471 ± 857 mg L−1, and sulfate feed concentrations varying from 1,253 to 2,081 mg L−1. The maximum sulfate reduction rate (SRR) of 9.75 ± 0.23 mmol (L day)−1 was achieved at the high sulfate influent concentration and the amount of organics consumed was between 80–90%. Sulfide levels in the UASB bioreactor were consistently high for most of the experiment, averaging 516.6 ± 188.5 mg L−1. Interestingly, during the last month of operation when sulfide concentrations were highest the SRR continued to increase. It was estimated that 36% of the silage leachate carbon was used directly for sulfate reduction.

Effect of nitrite and nitrate on biogenic sulfide production in sewer biofilms determined by the use of microelectrodes

2003 ◽  
Vol 47 (11) ◽  
pp. 281-288 ◽  
Author(s):  
S. Okabe ◽  
T. Ito ◽  
H. Satoh ◽  
Y. Watanabe
Keyword(s):  
Sulfate Reduction ◽  
Reduction Rate ◽  
Rotating Disk ◽  
Sulfate Reduction Rate ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
H2s Oxidation ◽  
Rotating Disk Reactors ◽  
Microelectrode Measurements

The effects of O2 and NO3− concentrations on in situ sulfate reduction and sulfide reoxidation in microaerophilic wastewater biofilms grown on rotating disk reactors were investigated by the use of microelectrodes for O2, S2−, NO3−, NO2−, and pH. Microelectrode measurements showed the vertical microzonation of O2 respiration, NO3− respiration, H2S oxidation and SO42− reduction in the biofilms. The microelectrode measurements indicate that sulfate reducing activity was largely restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from the O2 and NO3− respiration zones. Anaerobic H2S oxidation with NO3− was also induced by addition of nitrate to the medium. Measurements of the reduced inorganic sulfur compounds (FeS, FeS2 and S0), total-Mn and total-Fe in the biofilm indicated that the produced H2S became immediately oxidized with O2, NO3− and other oxidants, mainly ferric/ferrous hydrates. On the basis of the present results, it was estimated that of all sulfide produced, 13% of the sulfide was precipitated by metal ions as FeS and S0 just above the sulfate reduction zone, 65% was anaerobically oxidized to SO42− with NO3− as an electron acceptor and 22% was aerobically oxidized within the biofilm incubated in 70 μmol l−1 of DO and 280 μmol l−1 of NO3−.

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