scholarly journals Transcriptome analysis reveals key genes associated with root-lesion nematode Pratylenchus thornei resistance in chickpea

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sonal Channale ◽  
Danamma Kalavikatte ◽  
John P. Thompson ◽  
Himabindu Kudapa ◽  
Prasad Bajaj ◽  
...  
Keyword(s):  
Nematode Species ◽  
Post Inoculation ◽  
Root Lesion Nematode ◽  
Resistant Genotype ◽  
Functional Roles ◽  
Pratylenchus Thornei ◽  
Significant Yield ◽  
Lesion Nematode ◽  
Underlying Mechanisms ◽  
Moderately Resistant

AbstractThe root-lesion nematode, Pratylenchus thornei, is one of the major plant-parasitic nematode species causing significant yield losses in chickpea (Cicer arietinum). In order to identify the underlying mechanisms of resistance to P. thornei, the transcriptomes of control and inoculated roots of three chickpea genotypes viz. D05253 > F3TMWR2AB001 (resistant advanced breeding line), PBA HatTrick (moderately resistant cultivar), and Kyabra (susceptible cultivar) were studied at 20 and 50 days post inoculation using the RNA-seq approach. On analyzing the 633.3 million reads generated, 962 differentially expressed genes (DEGs) were identified. Comparative analysis revealed that the majority of DEGs upregulated in the resistant genotype were downregulated in the moderately resistant and susceptible genotypes. Transcription factor families WRKY and bZIP were uniquely expressed in the resistant genotype. The genes Cysteine-rich receptor-like protein kinase 10, Protein lifeguard-like, Protein detoxification, Bidirectional sugar transporter Sugars Will Eventually be Exported Transporters1 (SWEET1), and Subtilisin-like protease were found to play cross-functional roles in the resistant chickpea genotype against P. thornei. The identified candidate genes for resistance to P. thornei in chickpea can be explored further to develop markers and accelerate the introgression of P. thornei resistance into elite chickpea cultivars.

Metabolomic profiling of wheat genotypes resistant and susceptible to root-lesion nematode Pratylenchus thornei

2021 ◽  
Author(s):  
Md Motiur Rahaman ◽  
Rebecca S. Zwart ◽  
Thusitha W. T. Rupasinghe ◽  
Helen L. Hayden ◽  
John P. Thompson
Keyword(s):  
Root Lesion Nematode ◽  
Wheat Genotypes ◽  
Metabolomic Profiling ◽  
Pratylenchus Thornei ◽  
Lesion Nematode

Fine mapping of root lesion nematode (Pratylenchus thornei) resistance loci on chromosomes 6D and 2B of wheat

2019 ◽  
Vol 133 (2) ◽  
pp. 635-652 ◽  
Author(s):  
Muhammad Shefatur Rahman ◽  
Katherine J. Linsell ◽  
Julian D. Taylor ◽  
Matthew J. Hayden ◽  
Nicholas C. Collins ◽  
...  
Keyword(s):  
Fine Mapping ◽  
Root Lesion Nematode ◽  
Pratylenchus Thornei ◽  
Lesion Nematode

Pathogenicity of the root-lesion nematode, Pratylenchus zeae, on rice genotypes under different hydro-ecologies in Tanzania

Nematology ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 221-233 ◽  
Author(s):  
Yasinta Beda Nzogela ◽  
Sofie Landschoot ◽  
Ashura-Luzi Kihupi ◽  
Danny L. Coyne ◽  
Godelieve Gheysen
Keyword(s):  
Upland Rice ◽  
Growth And Yield ◽  
Post Inoculation ◽  
Root Lesion Nematode ◽  
Rice Genotypes ◽  
Lesion Nematode ◽  
The Impact ◽  
Mixed Stage ◽  
Pratylenchus Zeae ◽  
Better Than

Summary The root-lesion nematode, Pratylenchus zeae, is commonly found in upland rice fields. To measure the impact of the nematode on rice production, a screenhouse experiment was conducted using two farmer-adapted Oryza sativa cultivars, ‘Supa’ (‘SurinamV-880’) and ‘SARO-5’ (‘TXD 306’), under flooded, upland and drought water regimes imposed at 7 days post-inoculation of mixed-stage nematodes (200, 500, 1000, 3000 and 10 000 plant−1). Growth and yield parameters were recorded, and the experiment was terminated after 5 months. ‘Supa’ was shown to be resistant to P. zeae, while ‘SARO-5’ was susceptible. Pratylenchus zeae reduced the growth and yield of both cultivars, though more for ‘SARO-5’ than for ‘Supa’. Yield decreased with increasing final nematode densities. Pratylenchus zeae reproduction was highest at 200 and 500 inoculum levels and under upland water conditions. The yield of ‘SARO-5’ was greater than that of ‘Supa’ under flooded conditions and with no or 200 and 500 nematode inoculum levels, but with high P. zeae inoculum ‘Supa’ yield was better than ‘SARO-5’.

scholarly journals Pathotypes Detected Among Populations of Pratylenchus neglectus Collected From Montana

Plant Disease ◽  
2019 ◽  
Vol 103 (12) ◽  
pp. 3259-3264
Author(s):  
Riyadh T. Al-Khafaji ◽  
Erin E. Gunnink Troth ◽  
Kris N. Lambert ◽  
Jeffrey A. Johnston ◽  
Alan T. Dyer
Keyword(s):  
Population Analysis ◽  
The State ◽  
Crop Rotations ◽  
Root Lesion Nematode ◽  
Pratylenchus Neglectus ◽  
Changing Trends ◽  
Lesion Nematode ◽  
Initial Survey ◽  
Moderately Resistant ◽  
The Impact

The root lesion nematode, Pratylenchus neglectus, is one of the most damaging nematodes to affect wheat worldwide. The nematode is widely distributed in Montana, primarily affecting winter wheat within the state. Managing the nematode primarily involves rotation to resistant and moderately resistant crops (peas, lentils, and barley). A nematode survey was conducted across the state nearly 10 years after an initial survey, to reassess the nematode threat and assess the impact of changing trends in crop rotations. To assess the broad applicability of rotation crops to control P. neglectus across Montana, greenhouse trials were conducted to challenge rotational crops using eight populations of P. neglectus collected from geographically diverse locations across the state. In the trials, conducted with four Montana crops, a significant interaction was detected between crop and nematode population (analysis of variance P < 0.001). Populations from Hill, Dawson, and Chouteau counties were found to be pathogenic on barley. Male nematodes were detected in seven of the eight pot culture populations, and these were confirmed to be P. neglectus by morphological and molecular methods. These results suggest a re-evaluation of barley and lentils as a management option for P. neglectus in Montana, as pathotypes for each exist within the state.

Occurrence and abundance of nematodes on onion in Turkey and their relationship with soil physicochemical properties

Nematology ◽  
2019 ◽  
Vol 21 (10) ◽  
pp. 1063-1079
Author(s):  
Elif Yavuzaslanoglu ◽  
Ozlem Ates Sonmezoglu ◽  
Nimet Genc ◽  
Z. Mutlu Akar ◽  
Atilla Ocal ◽  
...  
Keyword(s):  
Large Scale ◽  
Calcium Content ◽  
Nematode Species ◽  
Free Living ◽  
Plant Feeding ◽  
Pratylenchus Thornei ◽  
Geographical Regions ◽  
Ditylenchus Dipsaci ◽  
Lesion Nematode ◽  
Production Areas

Summary The distribution of plant-feeding and free-living nematodes in large scale onion production areas in five geographical regions in Turkey was investigated in 2016 and 2017. Ditylenchus spp. and Tylenchus spp. were widely distributed. The stem and bulb nematode, Ditylenchus dipsaci, was found in 48 locations from 13 provinces. Other plant-feeding nematode genera were Pratylenchus, Paratylenchus and Pratylenchoides. Pratylenchus thornei was the most widely distributed root-lesion nematode species in onion fields in 11 locations from seven provinces. Pratylenchus neglectus was present in three locations and P. vulnus was in four locations. Aphelenchus spp. and Aphelenchoides spp. were the principal fungal-feeding nematodes in onion-growing areas. The most abundant bacterial-feeding nematode genera were Acrobeloides, Cephalobus, Eucephalobus and Rhabditis. Acrobeles and Wilsonema genera were low in occurrence and abundance. Nematodes from Dorylaimida and predator nematodes, Mononchus spp., were also found. The numbers of Ditylenchus from plant samples were significantly correlated positively to silt content, and significantly correlated negatively to organic matter and calcium content.

scholarly journals Saccharum spontaneum, a Novel Source of Resistance to Root-Lesion and Root-Knot Nematodes in Sugarcane

Plant Disease ◽  
2019 ◽  
Vol 103 (9) ◽  
pp. 2288-2294
Author(s):  
Shamsul A. Bhuiyan ◽  
Kylie Garlick ◽  
George Piperidis
Keyword(s):  
Nematode Species ◽  
Root Lesion Nematode ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Nematode Reproduction ◽  
Final Population ◽  
Lesion Nematodes ◽  
Commercial Cultivars ◽  
Lesion Nematode ◽  
Root Lesion Nematodes

Root-lesion nematode (Pratylenchus zeae) and root-knot nematode (Meloidogyne javanica) are two important pathogens of sugarcane (Saccharum hybrid). No commercial cultivars are resistant to these nematodes in Australia. Twenty accession lines of S. spontaneum, a wild relative of sugarcane, were tested against these two nematode species. S. spontaneum lines were tested twice for resistance to root-lesion nematode and three times for root-knot nematode. Reproduction (final population/starting population) of root-lesion nematodes was significantly lower in 17 of the 20 S. spontaneum accession lines tested in two experiments compared with two commercial cultivars. Four S. spontaneum lines supported a significantly lower number of root-lesion nematodes per gram of root than that of two commercial sugarcane cultivars. Reproduction of root-knot nematodes was significantly lower in 16 S. spontaneum lines compared with two commercial cultivars. Fourteen of the S. spontaneum lines tested supported significantly fewer eggs per gram of root compared with two commercial cultivars. This study showed that S. spontaneum lines possessed resistance for root-lesion and root-knot nematodes. Targeted crossing with commercial hybrid parental lines should be conducted to introduce nematode resistance into sugarcane cultivars for the Australian sugar industry.

A triumph of tolerance: managing the threat to wheat production by the root lesion nematode Pratylenchus thornei in the subtropical grain region of eastern Australia.

2021 ◽  
pp. 13-19
Author(s):  
Kirsty Owen
Keyword(s):  
Management Practices ◽  
Resistance Breeding ◽  
Nematode Resistance ◽  
Future Research ◽  
Root Lesion Nematode ◽  
Wheat Production ◽  
Production Region ◽  
Pratylenchus Thornei ◽  
Eastern Australia ◽  
Lesion Nematode

Abstract This chapter provides information on the economic importance, host range, geographical distribution, damage symptoms, biology and life cycle and interactions with other nematodes and pathogens of the root lesion nematode, Pratylenchus thornei, a severe and widespread threat to wheat production in the subtropical grain production region of eastern Australia. Some recommended integrated nematode management practices and future research for nematode resistance breeding are also presented.

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