scholarly journals Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Simone Morris ◽  
Pradeep Manuneedhi Cholan ◽  
Warwick J. Britton ◽  
Stefan H. Oehlers
Keyword(s):  
Immune Cells ◽  
Egg Yolk ◽  
High Fat ◽  
Zebrafish Larvae ◽  
Chicken Egg ◽  
Exogenous Glucose ◽  
Glucose Injection ◽  
Chicken Egg Yolk ◽  
Plug Formation ◽  
Glucose Supplementation

AbstractHyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.

scholarly journals Glucose inhibits neutrophil migration and haemostasis, and accelerates diet-induced hyperlipidaemia in zebrafish embryos

2021 ◽  
Author(s):  
Simone Morris ◽  
Pradeep Cholan ◽  
Warwick J Britton ◽  
Stefan H Oehlers
Keyword(s):  
Zebrafish Embryo ◽  
Neutrophil Migration ◽  
Egg Yolk ◽  
Zebrafish Embryos ◽  
High Fat ◽  
Exogenous Glucose ◽  
Chicken Egg Yolk ◽  
Plug Formation ◽  
Effect Of Glucose ◽  
Glucose Supplementation

ABSTRACTHyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish embryos to model the effect of glucose on leukocyte recruitment, thrombosis, and hyperlipidaemia by treating embryos with exogenous glucose. Exogenous glucose supplementation reduced neutrophil recruitment, and thrombocyte and fibrin plug formation following tail transection in zebrafish embryos, but did not affect the recruitment of macrophages. We observed accelerated lipid accumulation in glucose-supplemented embryos challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose in the tractable zebrafish embryo model system compared to mammals.

Chicken egg yolk antibodies (IgY)-based antivenom for neutralization of snake venoms: a review

Toxin Reviews ◽  
2021 ◽  
pp. 1-12
Author(s):  
Ankit Choraria ◽  
Rajeswari Somasundaram ◽  
S. Janani ◽  
Selvakumar Rajendran ◽  
Naoual Oukkache ◽  
...  
Keyword(s):  
Egg Yolk ◽  
Snake Venoms ◽  
Chicken Egg ◽  
Egg Yolk Antibodies ◽  
Chicken Egg Yolk

Development of viper-venom antibodies in chicken egg yolk and assay of their antigen binding capacity

Toxicon ◽  
2002 ◽  
Vol 40 (7) ◽  
pp. 857-861 ◽  
Author(s):  
C Maya Devi ◽  
M Vasantha Bai ◽  
L.K Krishnan
Keyword(s):  
Binding Capacity ◽  
Egg Yolk ◽  
Antigen Binding ◽  
Chicken Egg ◽  
Chicken Egg Yolk

Isolation of Immunoglobulin from Chicken Egg Yolk using Single-Stage Ultrafiltration with 100-kDa Regenerated Cellulose Membranes

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jianguo Liu ◽  
Juan Yang ◽  
Hai Xu ◽  
Hu Zhu ◽  
Jianbo Qu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Egg Yolk ◽  
Single Stage ◽  
Regenerated Cellulose ◽  
Cellulose Membrane ◽  
Permeate Flux ◽  
Operational Parameters ◽  
Solution Ph ◽  
Chicken Egg ◽  
Chicken Egg Yolk

The aim of this work is to develop a membrane-based cost-effective process for the rapid isolation of immunoglobulin from chicken egg yolk. It was found that a single-stage ultrafiltration using a 100 kDa molecular weight cut-off regenerated cellulose membrane could be employed to isolate immunoglobulin from the crude feedstock. The effects of operational parameters (solution pH, ionic strength, stirring speed and permeate flux) on the transmission of immunoglobulin and the presence of impurity protein with molecular weight close to immunoglobulin were quantified using the parameter scanning ultrafiltration technique. Under optimized conditions, the purity of immunoglobulin obtained was about 85 percent after the single-stage ultrafiltration process, and the recovery of immunoglobulin from the feedstock was 91 percent.

scholarly journals A simple method for isolating chicken egg yolk immunoglobulin using effective delipidation solution and ammonium sulfate

2015 ◽  
Vol 94 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Chenyao Tong ◽  
Fang Geng ◽  
Zhenjiao He ◽  
Zhaoxia Cai ◽  
Meihu Ma
Keyword(s):  
Ammonium Sulfate ◽  
Egg Yolk ◽  
Simple Method ◽  
Chicken Egg ◽  
Egg Yolk Immunoglobulin ◽  
Chicken Egg Yolk

scholarly journals COMPARISON OF ANTIVENOM POTENTIAL OF CHICKEN EGG YOLK ANTIBODIES GENERATED AGAINST BENTONITE AND ADJUVENT COATED VENOMES OF COMMON POISONOUS SNAKE IN INDIA

1970 ◽  
Vol 7 (1) ◽  
pp. 259-267 ◽  
Author(s):  
S. Meenatchisundaram ◽  
R. Selvakumaran ◽  
G. Parameswari ◽  
A. Michael
Keyword(s):  
Room Temperature ◽  
Deae Cellulose ◽  
Egg Yolk ◽  
Precipitation Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Snake Venoms ◽  
Chicken Egg ◽  
Ammonium Sulphate Precipitation ◽  
Egg Yolk Antibodies ◽  
Chicken Egg Yolk

Antivenom antibodies were generated in white leghorn chicken against bentonite and adjuvant coated venoms of Common Indian Poisonous Snakes (Cobra, Krait, Russell's viper and Saw Scaled viper).The antivenom from immunized chicken egg yolk were purified by polyethylene glycol (PEG) and ammonium sulphate precipitation method and further purified by DEAE cellulose ion exchange column chromatography and concentrated by polyvinyl pyrolidone powder at room temperature. The titer of antibodies was estimated using Enzyme Linked Immunosorbent Assay (ELISA).The chickens immunized with Freund's complete adjuvant showed slightly higher titre when compared to bentonite. Inhibition of lethal, edema, haemorrhagic, procoagulant and phospholipase A2 and fibrinolytic activities of snake venoms were determined. The chicken egg yolk antivenom was effective in neutralization of these toxic and enzymatic activities of venom. The median effective dose (ED50) of chicken egg yolk antibodies raised against adjuvant coated venoms showed effective neutralizing venom toxicity when compared to the antibodies raised using bentonite coated venoms.

scholarly journals EVALUATION OF CULTURE SUBSTRATE FOR THE ISOLATION AND CULTIVATION OF LEGIONELLA WITH THE USE OF THE STRAINS ISOLATED DURING THE XXII OLYMPIC GAMES-2014 IN SOCHI

2017 ◽  
pp. 49-50
Author(s):  
N.V. Zharinova ◽  
E.B. Zhilchenko ◽  
L.S. Katunina ◽  
O.A. Konyaeva ◽  
N.S. Serdyuk ◽  
...  
Keyword(s):  
Experimental Evaluation ◽  
Olympic Games ◽  
Egg Yolk ◽  
Enzymatic Hydrolysate ◽  
Long Period ◽  
Chicken Egg ◽  
Environmental Objects ◽  
Culture Substrate ◽  
Chicken Egg Yolk ◽  
And Storage

The article presents the results of experimental evaluation of the effectiveness of new culture substrate developed for the isolation and cultivation of Legionella, the strains isolated from environmental objects in the resort city of Sochi before and during XXII Olympic games. Dense nutrient substrate prepared on the basis of enzymatic hydrolysate of chicken egg yolk and enzymatic hydrolysate of the lung of the pig with additional stimulants allowed saving number of colonies in the earlier stages of the study and storage of the isolated strains during long period of time compared with domestic commercial nutrient substrate.

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