Vanilloid receptors on capsaicin-sensitive sensory nerves mediate relaxation to methanandamide in the rat isolated mesenteric arterial bed and small mesenteric arteries

The effects of hibernation on mesenteric arterial innervation and function were examined using pharmacological and immunohistochemical techniques in age-matched controls, cold-exposed controls, and 4-wk-hibernated golden hamsters. Electrical field stimulation of the isolated mesenteric arterial bed elicited frequency-dependent vasoconstriction. The sensitivity of responses was significantly increased in tissues from hibernating animals compared with cold-exposed controls. Vasoconstrictor responses to exogenous norepinephrine were also increased in hibernation. However, there was a significant decrease in sensitivity of vasoconstriction to ATP in hibernated and cold-exposed tissue compared with age-matched controls. In preparations preconstricted with methoxamine, endothelium-dependent vasodilator responses to acetylcholine and ATP were similar among the groups. Immunohistochemical investigation of mesenteric arteries revealed no differences among the groups in density of innervation by nerves immunoreactive for tyrosine hydroxylase, neuropeptide Y, and calcitonin gene-related peptide. Postjunctional changes appear to occur in hibernation, leading to augmentation of sympathetic vasoconstriction, which is consistent with the increase in peripheral vascular resistance in hibernation. Endothelium-dependent vasodilatation is not significantly changed in hibernation in the hamster mesenteric arterial bed.

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Effect of captopril on neurally induced contraction and relaxation of mesenteric arteries of renal hypertensive rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y00-011 ◽

2000 ◽

Vol 78 (6) ◽

pp. 469-475 ◽

Cited By ~ 5

Author(s):

Carlos F Santos ◽

Eduardo B Coelho ◽

Maria Cristina O. Salgado

Keyword(s):

Perfusion Pressure ◽

Renal Hypertension ◽

Electrical Field Stimulation ◽

Mesenteric Arteries ◽

Hypertensive Rats ◽

Frequency Dependent ◽

Hypertensive Group ◽

Mesenteric Arterial Bed ◽

Captopril Treatment ◽

Neurogenic Vasodilation

The effect of captopril treatment on neurally induced vasoconstrictor and vasodilator responses was examined in the isolated mesenteric arterial bed from normotensive and one-kidney, one clip hypertensive (1K1C) rats. In isolated mesenteric beds, electrical field stimulation (EFS) of perivascular nerves at basal tone induced a frequency-dependent increase in perfusion pressure that was greater in preparations from hypertensive rats compared with those from normotensive rats. Captopril treatment was associated with a decrease in vasoconstrictor responses in the hypertensive group compared with its non-treated control. Responses to norepinephrine (320 ng) were greater in hypertensive than normotensive groups; captopril reduced this response only in the hypertensive group. In preconstricted mesenteric arteries perfused with solutions containing guanethidine (5 µM) and atropine (1 µM), EFS elicited a frequency-dependent decrease in perfusion pressure that was abolished by tetrodotoxin (1 µM). Vasodilator responses to EFS were not affected by captopril treatment, although they were smaller in the hypertensive group. Acetylcholine (10 ng) induced similar decreases in perfusion pressure of normotensive and 1K1C groups; captopril did not influence these responses. These results indicate that captopril treatment does not affect the reduced neurogenic vasodilation but normalizes the augmented sympathetic-mediated vasoconstrictor responses of mesenteric resistance vessels of chronic 1K1C hypertensive rats.Key words: neurogenic vasodilation, calcitonin gene-related peptide, angiotensin, renal hypertension, angiotensin converting enzyme.

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Interaction between sympathetic and sensory nerves in rat small arteries: involvement of nitric oxide

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1996.271.3.h969 ◽

1996 ◽

Vol 271 (3) ◽

pp. H969-H976 ◽

Cited By ~ 2

Author(s):

A. Ahluwalia ◽

P. Vallance

Keyword(s):

Nitric Oxide ◽

Electrical Field Stimulation ◽

Ruthenium Red ◽

Sensory Nerves ◽

Mesenteric Arteries ◽

Resistance Arteries ◽

C Fibers ◽

Endothelial Denudation ◽

Cgrp Receptor Antagonist ◽

Endothelial Nitric Oxide

The aim of this study was to determine the effects of C-fiber activation on the reactivity of resistance arteries. Rat small mesenteric arteries were mounted in a tension myograph. Electrical field stimulation (EFS) of vessels produced brief contractions that were abolished by tetrodotoxin (1 microM, n = 4) or guanethidine (5 microM, n = 4). Capsaicin caused concentration-related attenuation of the EFS contraction [giving EFS responses of 61.7 +/- 4.3, 39.8 +/- 5.9, and 14.0 +/- 3.9% (n = 13-16) of control EFS contraction in the presence of 1, 3, and 10 microM capsaicin, respectively]. This effect was attenuated by the calcitonin gene-related peptide (CGRP) receptor antagonist CGRP-(8-37) (1 microM, n = 4, P < 0.05), NG-monomethyl-L-arginine (L-NMMA; 100 microM, n = 4, P < 0.01), or endothelial denudation (n = 5, P < 0.001). CGRP concentration-dependent inhibited (n = 5) EFS contraction, but this was unaffected by (L-NMMA (100 microM, n = 4). Capsaicin also relaxed preconstricted vessels (U-46619). This response was attenuated by ruthenium red (30 microM, n = 5, concentration ratio of 3.5 +/- 1.0) and CGRP-(8-37) (1 microM, n = 5, P < 0.05), while L-NMMA (100 microM, n = 6) showed variable effects, and denudation had no effect. These results show directly for the first time responses to activation of capsaicin-sensitive C fibers which modulate reactivity and the responses to sympathetic stimulation of isolated rat small mesenteric arteries, with the latter being an endothelial nitric oxide-dependent effect.

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Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide

Nature ◽

10.1038/22761 ◽

1999 ◽

Vol 400 (6743) ◽

pp. 452-457 ◽

Cited By ~ 1515

Author(s):

Peter M. Zygmunt ◽

Jesper Petersson ◽

David A. Andersson ◽

Huai-hu Chuang ◽

Morten Sørgård ◽

...

Keyword(s):

Sensory Nerves ◽

Vanilloid Receptors ◽

Vasodilator Action

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The effects of acute and chronic lipopolysaccharide infusion in rats on the efferent function of sensory nerves in the isolated mesenteric arterial bed

Autonomic Neuroscience ◽

10.1016/s1566-0702(03)00019-5 ◽

2003 ◽

Vol 107 (2) ◽

pp. 105-110 ◽

Cited By ~ 2

Author(s):

Matthew R. Farmer ◽

Sheila M. Gardiner ◽

Vera Ralevic

Keyword(s):

Sensory Nerves ◽

Mesenteric Arterial Bed ◽

Efferent Function

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Membrane-associated microtubular areays induced in proximal myelinated processes of dorsal root ganglia by large doses of vitamin B6

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143468 ◽

1986 ◽

Vol 44 ◽

pp. 368-369

Author(s):

V.J. Montpetit ◽

S. Dancea ◽

L. Tryphonas ◽

D.F. Clapin

Keyword(s):

Animal Model ◽

Endoplasmic Reticulum ◽

Dorsal Root Ganglia ◽

Rough Endoplasmic Reticulum ◽

Dorsal Root ◽

Vitamin B6 ◽

Morphological Changes ◽

Model System ◽

Sensory Nerves ◽

Peripheral Sensory

Very large doses of pyridoxine (vitamin B6) are neurotoxic in humans, selectively affecting the peripheral sensory nerves. We have undertaken a study of the morphological and biochemical aspects of pyridoxine neurotoxicity in an animal model system. Early morphological changes in dorsal root ganglia (DRG) associated with pyridoxine megadoses include proliferation of neurofilaments, ribosomes, rough endoplasmic reticulum, and Golgi complexes. We present in this report evidence of the formation of unique aggregates of microtubules and membranes in the proximal processes of DRG which are induced by high levels of pyridoxine.

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Glucuronyl 3-sulfate occurs exclusively on distal unmyelinated terminals of selected sensory neurons in the peripheral nervous system

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141160 ◽

1995 ◽

Vol 53 ◽

pp. 958-959

Author(s):

S.S. Spicer ◽

B.A. Schulte

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Cerebral Cortex ◽

Peripheral Nervous System ◽

Sensory Neurons ◽

Sensory Nerves ◽

Tissue Antigens ◽

The Central Nervous System ◽

The Brain ◽

Leu 7

Generation of monoclonal antibodies (MAbs) against tissue antigens has yielded several (VC1.1, HNK- 1, L2, 4F4 and anti-leu 7) which recognize the unique sugar epitope, glucuronyl 3-sulfate (Glc A3- SO4). In the central nervous system, these MAbs have demonstrated Glc A3-SO4 at the surface of neurons in the cerebral cortex, the cerebellum, the retina and other widespread regions of the brain.Here we describe the distribution of Glc A3-SO4 in the peripheral nervous system as determined by immunostaining with a MAb (VC 1.1) developed against antigen in the cat visual cortex. Outside the central nervous system, immunoreactivity was observed only in peripheral terminals of selected sensory nerves conducting transduction signals for touch, hearing, balance and taste. On the glassy membrane of the sinus hair in murine nasal skin, just deep to the ringwurt, VC 1.1 delineated an intensely stained, plaque-like area (Fig. 1). This previously unrecognized structure of the nasal vibrissae presumably serves as a tactile end organ and to our knowledge is not demonstrable by means other than its selective immunopositivity with VC1.1 and its appearance as a densely fibrillar area in H&E stained sections.

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