scholarly journals Genetic differentiation and phylogenetic relationships among Greek Silurus glanis and Silurus aristotelis (Pisces, Siluridae) populations, assessed by PCR–RFLP analysis of mitochondrial DNA segments

Heredity ◽  
1999 ◽  
Vol 82 (5) ◽  
pp. 503-509 ◽  
Author(s):  
A Triantafyllidis ◽  
T J Abatzopoulos ◽  
P S Economidis
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Differentiation ◽  
Phylogenetic Relationships ◽  
Rflp Analysis ◽  
Silurus Glanis ◽  
Pcr Rflp

Identification of Hake Species (MerlucciusGenus) Using Sequencing and PCR−RFLP Analysis of Mitochondrial DNA Control Region Sequences

2001 ◽  
Vol 49 (11) ◽  
pp. 5108-5114 ◽  
Author(s):  
Javier Quinteiro ◽  
Rodrigo Vidal ◽  
Mónica Izquierdo ◽  
Carmen G. Sotelo ◽  
María José Chapela ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Control Region ◽  
Rflp Analysis ◽  
Mitochondrial Dna Control Region ◽  
Control Region Sequences ◽  
Pcr Rflp

scholarly journals Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA

2016 ◽  
Vol 8 (3) ◽  
pp. 362
Author(s):  
Fidia Fibriana ◽  
Lutfia Nur Hadiyanti
Keyword(s):  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Morphological Characteristics ◽  
Biology Education ◽  
Rflp Analysis ◽  
Restriction Pattern ◽  
Internal Transcribed Spacers ◽  
Central Java ◽  
Pcr Rflp ◽  
Pcr Products

<p>In this study, twenty local durian accessions obtained from Central Java in situ collection were characterized using the morphological characteristics and the restriction patterns which generated from the region spanning the internal transcribed spacers ITS LEU and ITS 4. Morphological characteristics of durian leaf, stem, tree, and fruit showed variations for the different accessions, whereas polymerase chain reaction (PCR) products of ribosomal DNA region showed a low length of variation. The size of the PCR products and the restriction analyses with the restriction endonucleases Bsp1431yielded a restriction pattern for each accessions. The results of this study can be utilized by local durian farmers as a preliminary reference for durian propagation. The data obtained need to be supported by further research using the other molecular markers to obtain more accurate data. The clear identity of durian species can help the management of propagation systems by farmers to get superior local durian.</p><p><strong>How to Cite</strong></p><p>Fibriana, F., &amp; Hadiyanti, L. N. (2016). Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 362-370. </p>

PCR-based genetic identification of marlin and other billfish

1998 ◽  
Vol 49 (5) ◽  
pp. 383 ◽  
Author(s):  
B. H. Innes ◽  
P. M. Grewe ◽  
R. D. Ward
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Test ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Genetic Identification ◽  
Dna Molecule ◽  
Pcr Rflp ◽  
D Loop ◽  
Specific Variation ◽  
Species Specific

A genetic test was developed for the identification of the six species of billfish found in Australian waters (black marlin, Indo–Pacific blue marlin, striped marlin, Indo–Pacific sailfish, shortbill spearfish and broadbill swordfish). The test was based on the PCR–RFLP analysis of a 1400 bp region of the mitochondrial DNA molecule, the d-loop, using four restriction enzymes (Hinf I, Rsa I and Sau3A I andTaq I). A total of 33 composite haplotypes were observed among 160 fish; all were species-specific. Three of the species—black marlin, striped marlin and broadbill swordfish—showed sufficient intra-specific variation to be useful in population structure analyses.

Sign in / Sign up

Export Citation Format

Share Document