Phylogenetic relationships of Sakhalin taimen Parahucho perryi inferred from PCR-RFLP analysis of mitochondrial DNA

In this study, twenty local durian accessions obtained from Central Java in situ collection were characterized using the morphological characteristics and the restriction patterns which generated from the region spanning the internal transcribed spacers ITS LEU and ITS 4. Morphological characteristics of durian leaf, stem, tree, and fruit showed variations for the different accessions, whereas polymerase chain reaction (PCR) products of ribosomal DNA region showed a low length of variation. The size of the PCR products and the restriction analyses with the restriction endonucleases Bsp1431yielded a restriction pattern for each accessions. The results of this study can be utilized by local durian farmers as a preliminary reference for durian propagation. The data obtained need to be supported by further research using the other molecular markers to obtain more accurate data. The clear identity of durian species can help the management of propagation systems by farmers to get superior local durian.How to CiteFibriana, F., & Hadiyanti, L. N. (2016). Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA. Biosaintifika: Journal of Biology & Biology Education, 8(3), 362-370.

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PCR-based genetic identification of marlin and other billfish

Marine and Freshwater Research ◽

10.1071/mf98007 ◽

1998 ◽

Vol 49 (5) ◽

pp. 383 ◽

Cited By ~ 8

Author(s):

B. H. Innes ◽

P. M. Grewe ◽

R. D. Ward

Keyword(s):

Mitochondrial Dna ◽

Genetic Test ◽

Rflp Analysis ◽

Restriction Enzymes ◽

Genetic Identification ◽

Dna Molecule ◽

Pcr Rflp ◽

D Loop ◽

Specific Variation ◽

Species Specific

A genetic test was developed for the identification of the six species of billfish found in Australian waters (black marlin, Indo–Pacific blue marlin, striped marlin, Indo–Pacific sailfish, shortbill spearfish and broadbill swordfish). The test was based on the PCR–RFLP analysis of a 1400 bp region of the mitochondrial DNA molecule, the d-loop, using four restriction enzymes (Hinf I, Rsa I and Sau3A I andTaq I). A total of 33 composite haplotypes were observed among 160 fish; all were species-specific. Three of the species—black marlin, striped marlin and broadbill swordfish—showed sufficient intra-specific variation to be useful in population structure analyses.

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PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies

Chinese Science Bulletin ◽

10.1360/03tb9098 ◽

2003 ◽

Vol 48 (5) ◽

pp. 465 ◽

Cited By ~ 2

Author(s):

Jianfeng ZHOU

Keyword(s):

Mitochondrial Dna ◽

Common Carp ◽

Cyprinus Carpio ◽

Rflp Analysis ◽

Genetic Discrimination ◽

Common Carp Cyprinus Carpio ◽

Pcr Rflp ◽

Carp Cyprinus Carpio

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Mitochondrial DNA Mutation Detection by Electrospray Mass Spectrometry

Clinical Chemistry ◽

10.1373/clinchem.2006.074823 ◽

2007 ◽

Vol 53 (2) ◽

pp. 195-203 ◽

Cited By ~ 12

Author(s):

Yun Jiang ◽

Thomas A Hall ◽

Steven A Hofstadler ◽

Robert K Naviaux

Keyword(s):

Mass Spectrometry ◽

Mitochondrial Dna ◽

Rflp Analysis ◽

Endocrine Disorders ◽

Ion Cyclotron Resonance ◽

Accurate Identification ◽

Mtdna Mutations ◽

Fticr Ms ◽

Pcr Rflp ◽

Mtdna Variants

Abstract Background: Mitochondrial DNA (mtDNA) mutations cause a large spectrum of clinically important neurodegenerative, neuromuscular, cardiovascular, and endocrine disorders. We describe the novel application of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR MS) to the rapid and accurate identification of pathogenic mtDNA variants. Methods: In a blinded study, we used ESI-FTICR MS to analyze 24 unrelated samples of total cellular DNA containing 12 mtDNA variants and compared the results with those obtained by conventional PCR-restriction fragment length polymorphism (PCR-RFLP) analysis and gel electrophoresis. Results: From the 24-sample blinded panel, we correctly identified 12 of the samples as bearing an mtDNA variant and found the remaining 12 samples to have no pathogenic variants. The correlation coefficient between the 2 methods for mtDNA variant detection was 1.0; there were no false positives or false negatives in this sample set. In addition, the ESI-FTICR method identified 4 single-nucleotide polymorphisms (SNP) that had previously been missed by standard PCR-RFLP analysis. Conclusions: ESI-FTICR MS is a rapid, sensitive, and accurate method for the identification and quantification of mtDNA mutations and SNPs.

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