scholarly journals Cuticular protein with a low complexity sequence becomes cross-linked during insect cuticle sclerotization and is required for the adult molt

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Seulgi Mun ◽  
Mi Young Noh ◽  
Neal T. Dittmer ◽  
Subbaratnam Muthukrishnan ◽  
Karl J. Kramer ◽  
...  
Keyword(s):  
Low Complexity ◽  
Insect Cuticle ◽  
Cuticular Protein ◽  
Cuticle Sclerotization

Biphenyltetrols and Dibenzofuranones from Oxidative Coupling of Resorcinols with 4-Alkylpyrocatechols: New Ciues to the Mechanism of Insect Cuticle Sclerotization

1991 ◽  
Vol 74 (6) ◽  
pp. 1205-1212 ◽  
Author(s):  
Merle Miessner ◽  
Orlando Crescenzi ◽  
Alessandra Napolitano ◽  
Giuseppe Prota ◽  
Svend Olav Andersen ◽  
...  
Keyword(s):  
Oxidative Coupling ◽  
Insect Cuticle ◽  
Cuticle Sclerotization

scholarly journals Comparative transcriptomic analysis and endocuticular protein gene expression of alate adults, workers and soldiers of the termite Reticulitermes aculabialis

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Humaira Rasheed ◽  
Chenxu Ye ◽  
Yufeng Meng ◽  
Yuehua Ran ◽  
Jing Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Structural Characteristics ◽  
Division Of Labour ◽  
Structural Proteins ◽  
Pcr Analysis ◽  
Insect Cuticle ◽  
Cuticular Protein ◽  
Qrt Pcr ◽  
Comparative Transcriptomic Analysis

Abstract Background The insect cuticle is mainly composed of exocuticle and endocuticle layers that consist of a large number of structural proteins. The thickness and synthesis of the exocuticle depend on different castes that perform various functions in alates, workers and soldiers. However, it is not clear whether the soft endocuticle is involved in the division of labour in termite colonies. To reveal the structural characteristics of the endocuticle in different castes, we investigated the thickness of endocuticle layers in alates, workers and soldiers of the termite Reticulitermes aculabialis, and then we sequenced their transcriptome and detected the endocuticle protein genes. The differential expression levels of the endocuticular protein genes were confirmed in the three castes. Results We found that there was a great difference in the thickness of the endocuticle among the alates, soldiers and workers. The thickest endocuticle layers were found in the heads of the workers 7.88 ± 1.67 μm. The endocuticle layer in the head of the workers was approximately three-fold and nine-fold thicker than that in the heads of soldiers and alates, respectively. The thinnest endocuticle layers occurred in the head, thorax and abdomen of alates, which were 0.86 ± 0.15, 0.76 ± 0.24 and 0.52 ± 0.17 μm thick, respectively, and had no significant differences. A total of 43,531,650 clean sequencing reads was obtained, and 89,475 unigenes were assembled. Of the 70 identified cuticular protein genes, 10 endocuticular genes that belong to the RR-1 family were selected. qRT-PCR analysis of the five endocuticular genes (SgAbd-2, SgAbd-9, Abd-5, SgAbd-2-like and Abd-4-like) revealed that the endocuticle genes were more highly expressed in workers than in soldiers and alates. Conclusion These results suggest that SgAbd and Abd are the key components of the endocuticle. We infer that the thicker endocuticle in workers is helpful for them to perform their functions against environmental stress.

Model Insect Cuticle Sclerotization: Reactions of Catecholamine Quinones with the Nitrogen-Centered Nucleophiles Imidazole andN-Acetylhistidine

1997 ◽  
Vol 25 (3) ◽  
pp. 179-202 ◽  
Author(s):  
Xin Huang ◽  
Rongda Xu ◽  
M.Dale Hawley ◽  
Karl J. Kramer
Keyword(s):  
Insect Cuticle ◽  
Cuticle Sclerotization

Model reactions for insect cuticle sclerotization: Participation of amino groups in the cross-linking of Manduca sexta cuticle protein MsCP36

2010 ◽  
Vol 40 (3) ◽  
pp. 252-258 ◽  
Author(s):  
Richard J. Suderman ◽  
Neal T. Dittmer ◽  
Karl J. Kramer ◽  
Michael R. Kanost
Keyword(s):  
Manduca Sexta ◽  
Cross Linking ◽  
Insect Cuticle ◽  
Amino Groups ◽  
Cuticle Sclerotization ◽  
Cuticle Protein ◽  
The Cross ◽  
Model Reactions

ChemInform Abstract: Biphenyltetrols and Dibenzofuranones from Oxidative Coupling of Resorcinols with 4-Alkylpyrocatechols: New Clues to the Mechanism of Insect Cuticle Sclerotization.

ChemInform ◽  
2010 ◽  
Vol 22 (48) ◽  
pp. no-no
Author(s):  
M. MIESSNER ◽  
O. CRESCENZI ◽  
A. NAPOLITANO ◽  
G. PROTA ◽  
S. O. ANDERSEN ◽  
...  
Keyword(s):  
Oxidative Coupling ◽  
Insect Cuticle ◽  
Cuticle Sclerotization

Insect Cuticle Sclerotization

1992 ◽  
Vol 37 (1) ◽  
pp. 273-302 ◽  
Author(s):  
T L Hopkins ◽  
K J Kramer
Keyword(s):  
Insect Cuticle ◽  
Cuticle Sclerotization

Reconstruction of Chitin-Protein Complexes Using Correlation Averaging Methods: Ovipositor of the Ichneumon Fly Megarhyssa

1980 ◽  
Vol 38 ◽  
pp. 38-39
Author(s):  
L. T. Germinario ◽  
J. Blackwell ◽  
J. Frank
Keyword(s):  
Protein Complexes ◽  
Hexagonal Lattice ◽  
Uranyl Acetate ◽  
Optical Diffraction ◽  
Insect Cuticle ◽  
High Dose ◽  
Wide Angle ◽  
X Ray ◽  
Averaging Methods ◽  
Digital Correlation

This report describes the use of digital correlation and averaging methods 1,2 for the reconstruction of high dose electron micrographs of the chitin-protein complex from Megarhyssa ovipositor. Electron microscopy of uranyl acetate stained insect cuticle has demonstrated a hexagonal array of unstained chitin monofibrils, 2.4−3.0 nm in diameter, in a stained protein matrix3,4. Optical diffraction Indicated a hexagonal lattice with a = 5.1-8.3 nm3 A particularly well ordered complex is found in the ovipositor of the ichneumon fly Megarhyssa: the small angle x-ray data gives a = 7.25 nm, and the wide angle pattern shows that the protein consists of subunits arranged in a 61 helix, with an axial repeat of 3.06 nm5.

Sign in / Sign up

Export Citation Format

Share Document