scholarly journals Propagation phasor approach for holographic image reconstruction

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Wei Luo ◽  
Yibo Zhang ◽  
Zoltán Göröcs ◽  
Alborz Feizi ◽  
Aydogan Ozcan

Abstract To achieve high-resolution and wide field-of-view, digital holographic imaging techniques need to tackle two major challenges: phase recovery and spatial undersampling. Previously, these challenges were separately addressed using phase retrieval and pixel super-resolution algorithms, which utilize the diversity of different imaging parameters. Although existing holographic imaging methods can achieve large space-bandwidth-products by performing pixel super-resolution and phase retrieval sequentially, they require large amounts of data, which might be a limitation in high-speed or cost-effective imaging applications. Here we report a propagation phasor approach, which for the first time combines phase retrieval and pixel super-resolution into a unified mathematical framework and enables the synthesis of new holographic image reconstruction methods with significantly improved data efficiency. In this approach, twin image and spatial aliasing signals, along with other digital artifacts, are interpreted as noise terms that are modulated by phasors that analytically depend on the lateral displacement between hologram and sensor planes, sample-to-sensor distance, wavelength, and the illumination angle. Compared to previous holographic reconstruction techniques, this new framework results in five- to seven-fold reduced number of raw measurements, while still achieving a competitive resolution and space-bandwidth-product. We also demonstrated the success of this approach by imaging biological specimens including Papanicolaou and blood smears.

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Yair Rivenson ◽  
Yichen Wu ◽  
Aydogan Ozcan

Abstract Recent advances in deep learning have given rise to a new paradigm of holographic image reconstruction and phase recovery techniques with real-time performance. Through data-driven approaches, these emerging techniques have overcome some of the challenges associated with existing holographic image reconstruction methods while also minimizing the hardware requirements of holography. These recent advances open up a myriad of new opportunities for the use of coherent imaging systems in biomedical and engineering research and related applications.


2017 ◽  
Author(s):  
Zihao Wang ◽  
Donghun Ryu ◽  
Kuan He ◽  
Roarke Horstmeyer ◽  
Aggelos Katsaggelos ◽  
...  

Nanoscale ◽  
2014 ◽  
Vol 6 (11) ◽  
pp. 5807-5812 ◽  
Author(s):  
Joseph Louis Ponsetto ◽  
Feifei Wei ◽  
Zhaowei Liu

Fluorescent imaging resolution down to 51 nm is shown by generating tunable localized plasmon excitations on a nano-antenna array.


2021 ◽  
Vol 11 ◽  
Author(s):  
Guang Zeng ◽  
Jiahui He ◽  
Wenjian Qin

Whole slide imaging enables scanning entire stained-glass slides with high resolution into digital images for the tissue morphology/molecular pathology assessment and analysis, which has increased in adoption for both clinical and research applications. As an alternative to conventional optical microscopy, lensfree holography imaging, which offers high resolution and a wide field of view (FOV) with digital focus, has been widely used in various types of biomedical imaging. However, accurate colour holographic imaging with pixel super-resolution reconstruction has remained a great challenge due to its coherent characteristic. In this work, we propose a wide-field pixel super-resolution colour lensfree microscopy by performing wavelength scanning pixel super-resolution and phase retrieval simultaneously on the three channels of red, green and blue (RGB), respectively. High-resolution RGB three-channel composite colour image is converted to the YUV space for separating the colour component and the brightness component, keeping the brightness component unchanged as well as enhancing the colour component through average filter, which not only eliminates the common rainbow artifacts of holographic colour reconstruction but also maintains the high-resolution details collected under different colour illuminations. We conducted experiments on the reconstruction of a USAF1951, stained lotus root and red bone marrow smear for performance evaluation of the spatial resolution and colour reconstruction with an imaging FOV >40 mm2.


2014 ◽  
Vol 39 (8) ◽  
pp. 1202-1213 ◽  
Author(s):  
Heng SU ◽  
Jie ZHOU ◽  
Zhi-Hao ZHANG

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Andreas Markwirth ◽  
Mario Lachetta ◽  
Viola Mönkemöller ◽  
Rainer Heintzmann ◽  
Wolfgang Hübner ◽  
...  

Abstract Super-resolved structured illumination microscopy (SR-SIM) is among the fastest fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-build instruments are able to deliver two-fold resolution enhancement with high acquisition speed. SR-SIM is usually a two-step process, with raw-data acquisition and subsequent, time-consuming post-processing for image reconstruction. In contrast, wide-field and (multi-spot) confocal techniques produce high-resolution images instantly. Such immediacy is also possible with SR-SIM, by tight integration of a video-rate capable SIM with fast reconstruction software. Here we present instant SR-SIM by VIGOR (Video-rate Immediate GPU-accelerated Open-Source Reconstruction). We demonstrate multi-color SR-SIM at video frame-rates, with less than 250 ms delay between measurement and reconstructed image display. This is achieved by modifying and extending high-speed SR-SIM image acquisition with a new, GPU-enhanced, network-enabled image-reconstruction software. We demonstrate high-speed surveying of biological samples in multiple colors and live imaging of moving mitochondria as an example of intracellular dynamics.


Author(s):  
Amin Enayati ◽  
Aleksi Tamminen ◽  
Juha Ala-Laurinaho ◽  
Antti V. Raisanen ◽  
Guy A. E. Vandenbosch ◽  
...  

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