AbstractMesenchymal stromal cells (MSCs) from a variety of tissue sources are widely investigated in clinical trials, and the MSCs are often administered immediately after thawing the cryopreserved product. While previous reports have examined the transcriptome of freshly-cultured MSCs from some tissues, little is known about the single-cell transcriptomic profiles of out-of-thaw MSCs from different tissue sources. Such understanding could help determine which tissue origins and delivery methods are best suited for specific indications. Here, we characterized cryopreserved MSCs, immediately post-thaw, from bone marrow (BM) and cord tissue (CT), using single-cell RNA sequencing (scRNA-seq). We show that out-of-thaw BM-vs. CT-MSCs have significant differences in gene expression. Gene-set enrichment analyses implied divergent functional potential. In addition, we show that MSC-batches can vary significantly in cell cycle status, suggesting different proliferative vs. immunomodulatory potentials. Our results provide a comprehensive single-cell transcriptomic landscape of clinically and industrially relevant MSC products.HighlightsSingle cell gene expression comparison between Bone-marrow derived MSCs and Cord-tissue derived MSCsDonor effects and cell heterogeneity on tissue-specific MSC gene expressionSingle Cell Pooling Enhances Differential Expression Analysis for Bone marrow and Cord tissue MSC samplesGene ontology reveals tissue specific unique molecular function and pathways
Tissue-specific Differentiation Potency of Mesenchymal Stromal Cells from Perinatal Tissues
