Genetic architecture and mechanism of seed number per pod in rapeseed: elucidated through linkage and near-isogenic line analysis

The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.

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Development of a near isogenic line of ‘Koshihikari’ with a seed dormancy gene and an evaluation of its resistance to heat-induced quality decline

Breeding Research ◽

10.1270/jsbbr.18.1 ◽

2016 ◽

Vol 18 (1) ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Asako Kobayashi ◽

Kazuhiko Sugimoto ◽

Takeshi Hayashi ◽

Motohiko Kondo ◽

Junya Sonoda ◽

...

Keyword(s):

Seed Dormancy ◽

Isogenic Line ◽

Near Isogenic Line ◽

Resistance To Heat

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Genetic Dissection of Seed Dormancy using Chromosome Segment Substitution Lines in Rice (Oryza sativa L.)

International Journal of Molecular Sciences ◽

10.3390/ijms21041344 ◽

2020 ◽

Vol 21 (4) ◽

pp. 1344 ◽

Cited By ~ 1

Author(s):

Shaowen Yuan ◽

Yuntong Wang ◽

Chaopu Zhang ◽

Hanzi He ◽

Sibin Yu

Keyword(s):

Seed Dormancy ◽

Genetic Architecture ◽

Oryza Sativa L ◽

Expression Profiles ◽

Chromosome Segment ◽

Isogenic Line ◽

Substitution Lines ◽

Chromosome Segment Substitution Lines ◽

Segment Substitution ◽

Seed Dormancy And Germination

Timing of germination determines whether a new plant life cycle can be initiated; therefore, appropriate dormancy and rapid germination under diverse environmental conditions are the most important features for a seed. However, the genetic architecture of seed dormancy and germination behavior remains largely elusive. In the present study, a linkage analysis for seed dormancy and germination behavior was conducted using a set of 146 chromosome segment substitution lines (CSSLs), of which each carries a single or a few chromosomal segments of Nipponbare (NIP) in the background of Zhenshan 97 (ZS97). A total of 36 quantitative trait loci (QTLs) for six germination parameters were identified. Among them, qDOM3.1 was validated as a major QTL for seed dormancy in a segregation population derived from the qDOM3.1 near-isogenic line, and further delimited into a genomic region of 90 kb on chromosome 3. Based on genetic analysis and gene expression profiles, the candidate genes were restricted to eight genes, of which four were responsive to the addition of abscisic acid (ABA). Among them, LOC_Os03g01540 was involved in the ABA signaling pathway to regulate seed dormancy. The results will facilitate cloning the major QTLs and understanding the genetic architecture for seed dormancy and germination in rice and other crops.

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