scholarly journals Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yunxing Zhang ◽  
Xiaojiao Han ◽  
Shuangshuang Chen ◽  
Liu Zheng ◽  
Xuelian He ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Abiotic Stresses ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Quantitative Real Time Pcr ◽  
Salix Matsudana ◽  
Suitable Reference ◽  
Selection Of

Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Mulberry (Morus alba L.) under different abiotic stresses

2019 ◽  
Vol 46 (2) ◽  
pp. 1809-1817 ◽  
Author(s):  
Pawan Shukla ◽  
Ramesha A. Reddy ◽  
Kangayam M. Ponnuvel ◽  
Gulab Khan Rohela ◽  
Aftab A. Shabnam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Analysis ◽  
Real Time Pcr ◽  
Abiotic Stresses ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Quantitative Real Time Pcr ◽  
Suitable Reference ◽  
Selection Of

scholarly journals Selection of appropriate reference genes for quantitative real-time PCR in Clerodendrum trichotomum

2019 ◽  
Author(s):  
Yajie Hua ◽  
Yuanzheng Yue ◽  
Gongwei Chen ◽  
Taotao Yan ◽  
Wenjie Ding ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Quantitative Real Time Pcr ◽  
Mechanism Research ◽  
The Stability ◽  
Suitable Reference ◽  
Important Medicinal Plant ◽  
Selection Of

AbstrsctClerodendrum trichotomum, an important medicinal plant, has excellent salt tolerance and beautiful ornamental character. However, reliable reference genes for quantitative real-time PCR data (qRT-PCR) in C. trichotomum have not been investigated. Using our previous transcriptome data, 17 reference genes were selected in different tissues (leaves, flowers, fruits, stems, and roots) and under various abiotic stresses (salt, drought, flood, and heat) for C. trichotomum, using four different reference gene analysis software types: GeNorm, NormFinder, BestKeeper and ReFinder. The results identified RPL, ACT and HSP70 as the three most suitable reference genes for tissues. Genes ACT and AP-2 were most stably expressed under drought stress; MDH and UBCE2 were stable under flooding stress; RPL and UBCE2 were most stable under salt stress; and MDH and EF-1A were most appropriate under heat stress. For abiotic treatments, RPL, MDH and AP-2 were the most stable reference genes; and AP-2, RPL and ACT were stably expressed in all examined samples. The expression profile of the genes for Na+/H+ Exchanger1 (ClNHX1) and laccase (ClLAC) were selected to validate the stability of the determined reference genes. Our study provided reliable normalization for gene expression analysis and ensured more accurate data for further molecular mechanism research in C. trichotomum.

Selection of suitable reference genes for reverse transcription quantitative real-time PCR studies on different experimental systems from carrot (Daucus carota L.)

2015 ◽  
Vol 186 ◽  
pp. 115-123 ◽  
Author(s):  
Maria Doroteia Campos ◽  
António Miguel Frederico ◽  
Thomas Nothnagel ◽  
Birgit Arnholdt-Schmitt ◽  
Hélia Cardoso
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Daucus Carota ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Daucus Carota L ◽  
Experimental Systems ◽  
Suitable Reference ◽  
Selection Of

scholarly journals Selection of Suitable Reference Genes for Quantitative Real-time PCR in Sapium sebiferum

2017 ◽  
Vol 8 ◽  
Author(s):  
Xue Chen ◽  
Yingji Mao ◽  
Shengwei Huang ◽  
Jun Ni ◽  
Weili Lu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Sapium Sebiferum ◽  
Quantitative Real Time Pcr ◽  
Suitable Reference ◽  
Selection Of

scholarly journals Selection of appropriate reference genes for the detection of rhythmic gene expression via quantitative real-time PCR in Tibetan hulless barley

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190559 ◽  
Author(s):  
Jing Cai ◽  
Pengfei Li ◽  
Xiao Luo ◽  
Tianliang Chang ◽  
Jiaxing Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Hulless Barley ◽  
Rhythmic Gene ◽  
Tibetan Hulless Barley ◽  
Selection Of
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