A Novel Impedimetric Microfluidic Analysis System for Transgenic Protein Cry1Ab Detection

Microfluidic devices are of considerable interest, since such technology offers great promise for the development of powerful and versatile miniaturized analyzers. Accordingly, the present work describes a microfluidic screening system that is composed of a microchip, hydrodynamic pumping unit and fluorescence detectors. To develop an assay for RNA-aminoglycoside interactions, microchips are designed and fabricated on a glass substrate, then flow simulations are performed in the microchannels. After optimizing the flow control and buffer composition for fluorescence-based biochemical assays, a fluorescently labeled aminoglycoside probe and RNA are allowed to flow continuously to the main micro-channel based on hydrodynamic pumping and their interactions monitored by fluorescence quenching, which is reversed upon competition with other aminoglycosides. Consequently, the proposed device can serve as an integrated microfluidic platform for the high-throughput screening of high affinity antibiotics for RNA targets.

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Development of a new microfluidic analysis system based on porous silicon as sensitive element

physica status solidi (c) ◽

10.1002/pssc.200674388 ◽

2007 ◽

Vol 4 (6) ◽

pp. 2093-2097 ◽

Cited By ~ 5

Author(s):

Mihaela Miu ◽

Anca Angelescu ◽

Irina Kleps ◽

Florea Craciunoiu ◽

Monica Simion ◽

...

Keyword(s):

Porous Silicon ◽

Sensitive Element ◽

Microfluidic Analysis ◽

Analysis System

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CMAS: fully integrated portable centrifugal microfluidic analysis system for on-site colorimetric analysis

RSC Advances ◽

10.1039/c3ra42975j ◽

2013 ◽

Vol 3 (36) ◽

pp. 15928 ◽

Cited By ~ 27

Author(s):

Monika Czugala ◽

Damian Maher ◽

Fiachra Collins ◽

Robert Burger ◽

Frank Hopfgartner ◽

...

Keyword(s):

Colorimetric Analysis ◽

Fully Integrated ◽

Microfluidic Analysis ◽

Analysis System

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Development of a Multichannel Microfluidic Analysis System Employing Affinity Capillary Electrophoresis for Immunoassay

Analytical Chemistry ◽

10.1021/ac0007938 ◽

2001 ◽

Vol 73 (7) ◽

pp. 1472-1479 ◽

Cited By ~ 123

Author(s):

Siew Bang Cheng ◽

Cameron D. Skinner ◽

Justine Taylor ◽

Said Attiya ◽

William E. Lee ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Affinity Capillary Electrophoresis ◽

Microfluidic Analysis ◽

Analysis System

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Microfluidic Analysis System

Encyclopedia of Microfluidics and Nanofluidics ◽

10.1007/978-0-387-48998-8_925 ◽

2008 ◽

pp. 1136-1136 ◽

Cited By ~ 1

Keyword(s):

Microfluidic Analysis ◽

Analysis System

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Real-time neurochemical measurement of dynamic metabolic events during cardiac arrest and resuscitation in a porcine model

The Analyst ◽

10.1039/c9an01950b ◽

2020 ◽

Vol 145 (5) ◽

pp. 1894-1902

Author(s):

Sally A. N. Gowers ◽

Isabelle C. Samper ◽

De-Shaine R. K. Murray ◽

Georgia K. Smith ◽

Sarah Jeyaprakash ◽

...

Keyword(s):

Cardiac Arrest ◽

Real Time ◽

Porcine Model ◽

Real Time Monitoring ◽

Dynamic Changes ◽

Fully Integrated ◽

Microfluidic Analysis ◽

Analysis System ◽

The Brain

This work describes a fully-integrated portable microfluidic analysis system for real-time monitoring of dynamic changes in glucose and lactate occurring in the brain as a result of cardiac arrest and resuscitation.

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Automated Image Analysis Of Nuclear And Cytoplasmic Changes In Exfoliated Cells From Tracheas Treated With Carcinogen

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100078638 ◽

1977 ◽

Vol 35 ◽

pp. 220-221

Author(s):

S.F. Stinson ◽

J.C. Lilga ◽

M.B. Sporn

Keyword(s):

Image Analysis ◽

Pattern Analysis ◽

Relative Proportion ◽

Automated Image Analysis ◽

Image Analyzer ◽

Cross Sectional ◽

Exfoliated Cells ◽

Neoplastic Lesions ◽

Analysis System ◽

Morphologic Criterion

Increased nuclear size, resulting in an increase in the relative proportion of nuclear to cytoplasmic sizes, is an important morphologic criterion for the evaluation of neoplastic and pre-neoplastic cells. This paper describes investigations into the suitability of automated image analysis for quantitating changes in nuclear and cytoplasmic cross-sectional areas in exfoliated cells from tracheas treated with carcinogen.Neoplastic and pre-neoplastic lesions were induced in the tracheas of Syrian hamsters with the carcinogen N-methyl-N-nitrosourea. Cytology samples were collected intra-tracheally with a specially designed catheter (1) and stained by a modified Papanicolaou technique. Three cytology specimens were selected from animals with normal tracheas, 3 from animals with dysplastic changes, and 3 from animals with epidermoid carcinoma. One hundred randomly selected cells on each slide were analyzed with a Bausch and Lomb Pattern Analysis System automated image analyzer.

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