Design and Characterization of Microfluidic Analysis System for RNA-Aminoglycoside Interactions

Microfluidic devices are of considerable interest, since such technology offers great promise for the development of powerful and versatile miniaturized analyzers. Accordingly, the present work describes a microfluidic screening system that is composed of a microchip, hydrodynamic pumping unit and fluorescence detectors. To develop an assay for RNA-aminoglycoside interactions, microchips are designed and fabricated on a glass substrate, then flow simulations are performed in the microchannels. After optimizing the flow control and buffer composition for fluorescence-based biochemical assays, a fluorescently labeled aminoglycoside probe and RNA are allowed to flow continuously to the main micro-channel based on hydrodynamic pumping and their interactions monitored by fluorescence quenching, which is reversed upon competition with other aminoglycosides. Consequently, the proposed device can serve as an integrated microfluidic platform for the high-throughput screening of high affinity antibiotics for RNA targets.

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Synthesis and Characterization of Foamed Cassava Starch Material

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.262.405 ◽

2012 ◽

Vol 262 ◽

pp. 405-409

Author(s):

Yang Liu ◽

Shan Shan Li ◽

Xin Yan Yang ◽

Chong Xing Huang

Keyword(s):

Reaction Time ◽

Cassava Starch ◽

Synthesis And Characterization ◽

Compression Stress ◽

Static Compression ◽

Foaming Agent ◽

New Type ◽

Analysis System ◽

Central Composite

A new-type foam composites were fabricated by baking method from a mixture of cassava starch. A central composite design was constructed using the software Statistics Analysis System 9.0 to evaluate the static compression stress of foamed material. The optimum dosages of adhesive, foaming agent, catalyst were 2.0g, 6.0g, 2.4g, respectively; reaction temperature 65°C; reaction time 16 hour.

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Utilisation d'une ligne de microphotodiodes en spectroscopie dispersive : application à la détection du NO2

Canadian Journal of Physics ◽

10.1139/p83-040 ◽

1983 ◽

Vol 61 (2) ◽

pp. 301-304 ◽

Cited By ~ 3

Author(s):

Jacques Bures ◽

François Leonard ◽

Jean-Pierre Monchalin

Keyword(s):

Absorption Spectrum ◽

Systematic Errors ◽

Sufficient Information ◽

Atmospheric Pollutant ◽

Spatial Frequencies ◽

Low Pass ◽

On Line ◽

Photodiode Array ◽

Analysis System

A self-scanned photodiode array has been used as a multiplex sensor for laboratory detection and measurement, by dispersive spectroscopy, of trace quantities of the atmospheric pollutant NO2. The on-line data acquisition and numerical analysis system allows in particular to eliminate some systematic errors and drifts (Taylor filtering) and the noise associated with high spatial frequencies (low-pass filtering). We have then been able to show that an absorption spectrum, corresponding to low absorber concentrations, has a sufficient information content for the characterization of the pollutant and the measurement of its concentration (ppm m), even when noise and drifts are present. The proposed system can be favorably compared to the ones, based on a single photoelectric detector, which are commercially used.

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