Non-target arthropods may be affected by toxins derived from Bacillus thuringiensis (Bt) expressed in transgenic maize. The objective of this study was to evaluate the possible impacts of Bt maize on the diversity and the composition of non-target arthropod species by analyzing one field cultivated with conventional maize (no expressing transgenic protein) and three fields cultivated with transgenic maize (expressing Bt proteins). In each field was sampled 50 entirely plants for the number of arthropod specimens and registred the degree of injury caused by the chewing insects. A total of 2.525 specimens of arthropods, comprising 29 species from 25 families, were recorded on 3.000 sampled plants. The most diverse family belonged to the order Hemiptera. Based on Shannon and Simpson indexes, the Bt-transgenic cultivar EXP3320YG had lower level of non-target arthropod diversity than other cultivars. From this study, it is clear that the diversity of non-target arthropods on maize crop is negatively affected by Cry1Ab protein, while the Cry1A105+Cry2Ab2+Cry1F proteins, and Cry1A105+Cry2Ab2+Cry3Bb1 proteins do not have any effect on arthropod species diversity and composition.
Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein