Determination of Pb-bound ligands in human amniotic fluid by HPSEC-ICP-MS

1999 ◽  
Vol 36 (3) ◽  
pp. 93-95 ◽  
Author(s):  
Gene S. Hall ◽  
Xiangping Zhu ◽  
Eugene G. Martin
Keyword(s):  
Amniotic Fluid ◽  
Human Amniotic Fluid ◽  
Icp Ms

Determination of Bile Acid Concentration in Human Amniotic Fluid for Prenatal Diagnosis of Intestinal Obstruction

PEDIATRICS ◽  
1977 ◽  
Vol 59 (5) ◽  
pp. 647-650
Author(s):  
Guy Délèze ◽  
Dimitri Sidiropoulos ◽  
Gustav Paumgartner
Keyword(s):  
Bile Acid ◽  
Prenatal Diagnosis ◽  
Intestinal Obstruction ◽  
Amniotic Fluid ◽  
Acid Concentration ◽  
Total Bile Acid ◽  
Papilla Of Vater ◽  
Human Amniotic Fluid ◽  
Bile Acid Concentration

Bile acid concentration was measured in amniotic fluid obtained for standard indications from 11 healthy pregnant women without polyhydramnios (28 to 42 weeks of gestation) and from 9 patients with polyhydramnios (28 to 38 weeks of gestation). Two of the latter women delivered infants with intestinal obstruction distal to the papilla of Vater, a condition that causes regurgitation of bile into the amniotic fluid. In the women without polyhydramnios, the total bile acid concentration ranged from 1.4 to 2.4 µmol/liter. In the seven patients with polyhydramnios not associated with fetal intestinal obstruction, the bile acid concentration in amniotic fluid was not significantly different (0.9 to 1.9 µmol/liter). By contrast, the bile acid concentration in amniotic fluid specimens from the two patients with polyhydramnios who gave birth to children with intestinal obstruction was considerably elevated (30.3 and 83.1 µmol/liter). These findings suggest that determination of bile acid concentration in amniotic fluid permits prenatal diagnosis of intestinal obstruction distal to the papilla of Vater.

Determination of cocaine and benzoylecgonine in human amniotic fluid using high flow solid-phase extraction columns and HPLC

1992 ◽  
Vol 56 (2) ◽  
pp. 177-181 ◽  
Author(s):  
Christine Moore ◽  
Susan Browne ◽  
Ian Tebbett ◽  
Adam Negrusz ◽  
William Meyer ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Amniotic Fluid ◽  
Solid Phase ◽  
High Flow ◽  
Phase Extraction ◽  
Human Amniotic Fluid

scholarly journals Determination of bisphenol-A levels in human amniotic fluid samples by liquid chromatography coupled with mass spectrometry

2011 ◽  
Vol 34 (14) ◽  
pp. 1648-1655 ◽  
Author(s):  
Mei Chen ◽  
Andrea G. Edlow ◽  
Tao Lin ◽  
Nicole A. Smith ◽  
Thomas F. McElrath ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Bisphenol A ◽  
Amniotic Fluid ◽  
Human Amniotic Fluid

Methods for extracting phospholipids from human amniotic fluid compared.

1984 ◽  
Vol 30 (2) ◽  
pp. 271-274 ◽  
Author(s):  
C G Duck-Chong ◽  
G J Baker ◽  
S R Murdoch ◽  
R M Price
Keyword(s):  
Amniotic Fluid ◽  
Extraction Method ◽  
Phospholipid Composition ◽  
Fetal Lung ◽  
Absolute Concentration ◽  
Human Amniotic Fluid ◽  
Fetal Lung Maturity ◽  
Lung Maturity

Abstract Phospholipids were extracted from human amniotic fluid by various procedures, including the two most commonly applied to amniotic fluid for evaluation of fetal lung maturity. We find that the yield of phospholipid is greatly procedure dependent. This should be taken into account when one is considering the various reported methods of evaluating fetal lung maturity, because in some of them phospholipid data are expressed in terms of absolute concentration in the amniotic fluid. There were also significant differences in phospholipid composition in extracts prepared by the various procedures, but in general these were not large enough to influence evaluation of fetal lung maturity by methods in which phospholipid data are expressed in relative terms, as ratios or percentages--e.g., in the lecithin/sphingomyelin ratio and "lung profile" procedures. In the extraction method originally recommended for determination of the lecithin/sphingomyelin ratio, both the yield and composition of phospholipid depend on the extent of mixing.

scholarly journals Determination of bisphenol-A levels in human amniotic fluid samples by liquid chromatography coupled with mass spectrometry

10.1002/jssc. ◽  
2011 ◽  
pp. n/a-n/a
Author(s):  
Mei Chen ◽  
Andrea G. Edlow ◽  
Tao Lin ◽  
Nicole A. Smith ◽  
Thomas F. McElrath ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Bisphenol A ◽  
Amniotic Fluid ◽  
Human Amniotic Fluid

Liquid-chromatographic determination of bilirubin and its conjugates in rat serum and human amniotic fluid.

1981 ◽  
Vol 27 (10) ◽  
pp. 1704-1707 ◽  
Author(s):  
P Rosenthal ◽  
N Blanckaert ◽  
P M Kabra ◽  
M M Thaler
Keyword(s):  
Amniotic Fluid ◽  
High Performance ◽  
Methyl Esters ◽  
Chromatographic Determination ◽  
Unconjugated Bilirubin ◽  
Human Amniotic Fluid ◽  
Rat Serum ◽  
Alkaline Methanolysis ◽  
Liquid Chromatographic

Abstract This newly developed and highly specific and sensitive procedure was applied to the determination of unconjugated bilirubin and its ester conjugates in rat serum and human amniotic fluid. Bilirubin conjugates in biological samples are converted to methyl esters by alkaline methanolysis, extracted into chloroform, and the unconjugated bilirubin and esterified pigment derivatives are fractionated by "high-performance" liquid chromatography. The separated pigments are measured spectrophotometrically. Bilirubin and its mono- and di-conjugates are readily quantitated, even in previously undetectable concentrations. Linearity was established from 0.07 to 121.2 mumol/L for unconjugated bilirubin, 0.07 to 34.6 mumol/L for the C-8 monoconjugate, 0.06 to 69.3 mumol/L for the C-12 monoconjugate, and 0.17 to 43.7 mumol/L for the di-conjugate fraction. The detection limit was 0.03 mumol/L for unconjugated bilirubin and for each monoconjugate, and 0.1 mumol/L for the di-conjugated pigment.

scholarly journals Improved immunoassay for the determination of surfactant protein A (SP-A) in human amniotic fluid.

1989 ◽  
Vol 157 (3) ◽  
pp. 269-278 ◽  
Author(s):  
HIROSHI SHIMIZU ◽  
KENJI HOSODA ◽  
MASAHIKO MIZUMOTO ◽  
YOSHIO KUROKI ◽  
HIDEKI SATO ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Protein A ◽  
Surfactant Protein ◽  
Surfactant Protein A ◽  
Human Amniotic Fluid
Sign in / Sign up

Export Citation Format

Share Document