Simultaneous Determination of Phospholipid Classes and the Major Molecular Species of Lecithin in Human Amniotic Fluid by HPLC

Bile acid concentration was measured in amniotic fluid obtained for standard indications from 11 healthy pregnant women without polyhydramnios (28 to 42 weeks of gestation) and from 9 patients with polyhydramnios (28 to 38 weeks of gestation). Two of the latter women delivered infants with intestinal obstruction distal to the papilla of Vater, a condition that causes regurgitation of bile into the amniotic fluid. In the women without polyhydramnios, the total bile acid concentration ranged from 1.4 to 2.4 µmol/liter. In the seven patients with polyhydramnios not associated with fetal intestinal obstruction, the bile acid concentration in amniotic fluid was not significantly different (0.9 to 1.9 µmol/liter). By contrast, the bile acid concentration in amniotic fluid specimens from the two patients with polyhydramnios who gave birth to children with intestinal obstruction was considerably elevated (30.3 and 83.1 µmol/liter). These findings suggest that determination of bile acid concentration in amniotic fluid permits prenatal diagnosis of intestinal obstruction distal to the papilla of Vater.

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Determination of intramuscular phospholipid classes and molecular species in Gaoyou duck

Food Chemistry ◽

10.1016/j.foodchem.2008.05.052 ◽

2009 ◽

Vol 112 (1) ◽

pp. 150-155 ◽

Cited By ~ 27

Author(s):

Daoying Wang ◽

Weimin Xu ◽

Xinglian Xu ◽

Guanghong Zhou ◽

Yongzhi Zhu ◽

...

Keyword(s):

Molecular Species ◽

Phospholipid Classes

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Simultaneous Determination of Zalcitabine and Stavudine in Maternal Plasma, Amniotic Fluid, Placental, and Fetal Tissues using Reversed Phase on Silica Liquid Chromatography

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826070701812715 ◽

2008 ◽

Vol 31 (4) ◽

pp. 482-496 ◽

Cited By ~ 3

Author(s):

Meng Xu ◽

Catherine A. White ◽

Michael G. Bartlett

Keyword(s):

Liquid Chromatography ◽

Amniotic Fluid ◽

Simultaneous Determination ◽

Maternal Plasma ◽

Reversed Phase ◽

Fetal Tissues

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Determination of cocaine and benzoylecgonine in human amniotic fluid using high flow solid-phase extraction columns and HPLC

Forensic Science International ◽

10.1016/0379-0738(92)90176-w ◽

1992 ◽

Vol 56 (2) ◽

pp. 177-181 ◽

Cited By ~ 8

Author(s):

Christine Moore ◽

Susan Browne ◽

Ian Tebbett ◽

Adam Negrusz ◽

William Meyer ◽

...

Keyword(s):

Solid Phase Extraction ◽

Amniotic Fluid ◽

Solid Phase ◽

High Flow ◽

Phase Extraction ◽

Human Amniotic Fluid

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Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.

Clinical Chemistry ◽

10.1093/clinchem/24.7.1144 ◽

1978 ◽

Vol 24 (7) ◽

pp. 1144-1146 ◽

Cited By ~ 23

Author(s):

G R Gotelli ◽

R E Stanfill ◽

P M Kabra ◽

F A Farina ◽

L J Marton

Keyword(s):

Thin Layer ◽

Amniotic Fluid ◽

Simultaneous Determination ◽

Chromatographic Method ◽

White Background ◽

Molybdenum Blue ◽

Spray Reagent ◽

Single Dimension

Abstract We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.

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Importance of simultaneous determination of coproporphyrin and hemoglobin in contaminated amniotic fluid.

Clinical Chemistry ◽

10.1093/clinchem/32.5.877 ◽

1986 ◽

Vol 32 (5) ◽

pp. 877-878 ◽

Cited By ~ 10

Author(s):

J Francoual ◽

A Lindenbaum ◽

C Benattar ◽

M Dehan ◽

H Cohen ◽

...

Keyword(s):

Amniotic Fluid ◽

Simultaneous Determination ◽

Biochemical Analysis ◽

Reliable Method ◽

Visual Examination ◽

Mean Values ◽

The Mean ◽

Comparison Of The Results

Abstract To detect the presence of meconium and blood, we determined coproporphyrin and hemoglobin in 78 amniotic fluid samples, classified according to their visual aspect as clear (n = 45) or meconium-stained (n = 33). Among the 33 samples visually judged as meconial, a comparison of the results of their biochemical analysis with the mean values for clear amniotic fluids showed that only five of the 33 were contaminated solely with meconium. Seven others were exclusively bloody, and 19 were meconial and bloody. In two, the contaminant was neither meconium nor blood. Evidently, when the fluid is brownish, simple visual examination does not suffice for distinguishing meconium from old blood or other pigments. We propose a simple, rapid, and reliable method for simultaneously measuring contamination with meconium or blood.

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