Over the past two decades, there have been increased emphases on the topic of green chemistry and chemical processes. Utilization of non toxic chemicals, environmentally benign solvents, and renewable materials are some of the key issues that merit important consideration in a green synthetic strategy. The Datura Inoxia leaves possesses biomolecules such as cardiac glycosides, proteins, phenolic compounds, flavonoids and sugar, which could be used as reducing agent to react with ferrous and ferric ions and as scaffolds to direct the formation of Fe3O4 NPs in solution. To the best of our knowledge, the use of Dhatura innoxia plant extract at room temperature for the bio-reductive synthesis of Fe3O4 nanoparticles has not been reported. The formation of the Fe3O4 magnetic nanoparticles was first monitored using UV-Vis absorption spectroscopy. FT-IR spectroscopy and TGA/DTG analysis further confirms the formation of plant protein coated magnetite nanobio hybrid. The dried form of synthesized nanoparticles was further characterized using XRD, TEM.