Stable isotope labelling and FPLC–ICP-SFMS for the accurate determination of clinical iron status parameters in human serum

The Analyst ◽  
2008 ◽  
Vol 133 (3) ◽  
pp. 379 ◽  
Author(s):  
M. Estela del Castillo Busto ◽  
Maria Montes-Bayón ◽  
Jörg Bettmer ◽  
Alfredo Sanz-Medel
Keyword(s):  
Stable Isotope ◽  
Human Serum ◽  
Iron Status ◽  
Accurate Determination ◽  
Stable Isotope Labelling ◽  
Isotope Labelling

scholarly journals Natural isotope correction improves analysis of protein modification dynamics

2021 ◽  
Author(s):  
Jörn Dietze ◽  
Alienke van Pijkeren ◽  
Anna-Sophia Egger ◽  
Mathias Ziegler ◽  
Marcel Kwiatkowski ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Modification ◽  
High Resolution Mass Spectrometry ◽  
Accurate Determination ◽  
Mass Spectrometry Data ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Naturally Occurring ◽  
Correct Estimation

AbstractStable isotope labelling in combination with high-resolution mass spectrometry approaches are increasingly used to analyze both metabolite and protein modification dynamics. To enable correct estimation of the resulting dynamics, it is critical to correct the measured values for naturally occurring stable isotopes, a process commonly called isotopologue correction or deconvolution. While the importance of isotopologue correction is well recognized in metabolomics, it has received far less attention in proteomics approaches. Although several tools exist that enable isotopologue correction of mass spectrometry data, the majority is tailored for the analysis of low molecular weight metabolites. We here present PICor which has been developed for isotopologue correction of complex isotope labelling experiments in proteomics or metabolomics and demonstrate the importance of appropriate correction for accurate determination of protein modifications dynamics, using histone acetylation as an example.

A stable isotope labelling assisted LC-MS method for the determination of polyamines in micro-tissues of rice

2017 ◽  
Vol 9 (23) ◽  
pp. 3541-3548 ◽  
Author(s):  
Qiu-Yi Wang ◽  
Tiantian Ye ◽  
Shu-Jian Zheng ◽  
Er-Cui Ye ◽  
Ren-Qi Wang ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Rice Plant ◽  
Cadmium Stress ◽  
Plant Tissues ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Highly Sensitive

A stable isotope labelling assisted LC-MS method for highly sensitive quantitation of polyamines in micro-regions of rice plant tissues under cadmium stress.

Dynamic lipidomics with stable isotope labelling

2009 ◽  
Vol 877 (26) ◽  
pp. 2716-2721 ◽  
Author(s):  
Anthony D. Postle ◽  
Alan N. Hunt
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Labelling ◽  
Isotope Labelling

scholarly journals Stable isotope labelling in vivo as an aid to protein identification in peptide mass fingerprinting

PROTEOMICS ◽  
2002 ◽  
Vol 2 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Julie M. Pratt ◽  
Duncan H. L. Robertson ◽  
Simon J. Gaskell ◽  
Isabel Riba-Garcia ◽  
Simon J. Hubbard ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Protein Identification ◽  
Peptide Mass Fingerprinting ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Peptide Mass

scholarly journals The contributions of biosynthesis and acyl chain remodelling to the molecular species profile of phosphatidylcholine in yeast

2005 ◽  
Vol 33 (5) ◽  
pp. 1146-1149 ◽  
Author(s):  
H.A. Boumann ◽  
A.I.P.M. de Kroon
Keyword(s):  
Stable Isotope ◽  
Species Composition ◽  
Molecular Species ◽  
Acyl Chain ◽  
Membrane Lipid ◽  
Molecular Species Composition ◽  
Stable Isotope Labelling ◽  
Membrane Function ◽  
Isotope Labelling ◽  
Acyl Chains

Phosphatidylcholine (PC) is a very abundant membrane lipid in most eukaryotes, including yeast. The molecular species profile of PC, i.e. the ensemble of PC molecules with acyl chains differing in number of carbon atoms and double bonds, is important for membrane function. Pathways of PC synthesis and turnover maintain PC homoeostasis and determine the molecular species profile of PC. Studies addressing the processes involved in establishing the molecular species composition of PC in yeast using stable isotope labelling combined with detection by MS are reviewed.

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