Multiparameter fluorescence image spectroscopy to study molecular interactions

2009 ◽  
Vol 8 (4) ◽  
pp. 470 ◽  
Author(s):  
Stefanie Weidtkamp-Peters ◽  
Suren Felekyan ◽  
Andrea Bleckmann ◽  
Rüdiger Simon ◽  
Wolfgang Becker ◽  
...  
Keyword(s):  
Molecular Interactions ◽  
Fluorescence Image ◽  
Image Spectroscopy

scholarly journals Cancer Diagnostics by Multiparameter Fluorescence Image Spectroscopy: A Bioinformatic Classifier Trained on Cultured Immunostained Cells

2013 ◽  
Vol 104 (2) ◽  
pp. 342a
Author(s):  
Peter D. Zentis ◽  
Manuel Frohnapfel ◽  
Ville Rantanen ◽  
Stefanie Weidtkamp-Peters ◽  
Suren Felekyan ◽  
...  
Keyword(s):  
Cancer Diagnostics ◽  
Fluorescence Image ◽  
Image Spectroscopy

scholarly journals Multiparameter Fluorescence Image Spectroscopy

2011 ◽  
Vol 100 (3) ◽  
pp. 139a
Author(s):  
Ralf Kuehnemuth ◽  
Heike Hornen ◽  
Suren Felekyan ◽  
Stefanie Weidtkamp-Peters ◽  
Elisabeth Knust ◽  
...  
Keyword(s):  
Fluorescence Image ◽  
Image Spectroscopy

scholarly journals Guanylate binding proteins directly attack Toxoplasma gondii via supramolecular complexes

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Elisabeth Kravets ◽  
Daniel Degrandi ◽  
Qijun Ma ◽  
Thomas-Otavio Peulen ◽  
Verena Klümpers ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Parasitophorous Vacuole ◽  
Supramolecular Complexes ◽  
Parasitophorous Vacuole Membrane ◽  
Gtp Hydrolysis ◽  
Fluorescence Image ◽  
Vacuole Membrane ◽  
Live Cell Microscopy ◽  
Image Spectroscopy ◽  
Cell Microscopy

GBPs are essential for immunity against intracellular pathogens, especially for Toxoplasma gondii control. Here, the molecular interactions of murine GBPs (mGBP1/2/3/5/6), homo- and hetero-multimerization properties of mGBP2 and its function in parasite killing were investigated by mutational, Multiparameter Fluorescence Image Spectroscopy, and live cell microscopy methodologies. Control of T. gondii replication by mGBP2 requires GTP hydrolysis and isoprenylation thus, enabling reversible oligomerization in vesicle-like structures. mGBP2 undergoes structural transitions between monomeric, dimeric and oligomeric states visualized by quantitative FRET analysis. mGBPs reside in at least two discrete subcellular reservoirs and attack the parasitophorous vacuole membrane (PVM) as orchestrated, supramolecular complexes forming large, densely packed multimers comprising up to several thousand monomers. This dramatic mGBP enrichment results in the loss of PVM integrity, followed by a direct assault of mGBP2 upon the plasma membrane of the parasite. These discoveries provide vital dynamic and molecular perceptions into cell-autonomous immunity.

scholarly journals Characterizing Membrane Protein Interactions in Vivo by Multiparameter Fluorescence Image Spectroscopy

2014 ◽  
Vol 106 (2) ◽  
pp. 399a-400a
Author(s):  
Qijun Ma ◽  
Marc Somssich ◽  
Stefanie Weidtkamp-Peters ◽  
Yvonne Stahl ◽  
Suren Felekyan ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Interactions ◽  
Fluorescence Image ◽  
Image Spectroscopy

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

Fluorescence of BODIPY Dyes in Gas Phase at near Ambient Conditions

2020 ◽  
Author(s):  
Kseniya A. Mariewskaya ◽  
Denis Larkin ◽  
Yuri Samoilichenko ◽  
Vladimir Korshun ◽  
Alex Ustinov
Keyword(s):  
Gas Phase ◽  
Molecular Interactions ◽  
Atmospheric Pressure ◽  
Fluorescence Spectra ◽  
Visible Range ◽  
Intrinsic Properties ◽  
Ambient Conditions ◽  
Ideal Model ◽  
Molecular Fluorescence ◽  
Tesla Coil

Molecular fluorescence is a phenomenon that is usually observed in condensed phase. It is strongly affected by molecular interactions. The study of fluorescence spectra in the gas phase can provide a nearly-ideal model for the evaluation of intrinsic properties of the fluorophores. Unfortunately, most conventional fluorophores are not volatile enough to allow study of their fluorescence in the gas phase. Here we report very bright gas phase fluorescence of simple BODIPY dyes that can be readily observed at atmospheric pressure using conventional fluorescence instrumentation. To our knowledge, this is the first example of visible range gas phase fluorescence at near ambient conditions. Evaporation of the dye in vacuum allowed us to demonstrate organic molecular electroluminescence in gas discharge excited by electric field produced by a Tesla coil.

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