Heat-shock transformation of Escherichia coli in nanolitre droplets formed in a capillary-composited microfluidic device

2011 ◽  
Vol 3 (9) ◽  
pp. 1988 ◽  
Author(s):  
Jun Sha ◽  
Yaolei Wang ◽  
Jianchun Wang ◽  
Wenming Liu ◽  
Qin Tu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Microfluidic Device

Capillary-composited microfluidic device for heat shock transformation of Escherichia coli

2011 ◽  
Vol 112 (4) ◽  
pp. 373-378 ◽  
Author(s):  
Jun Sha ◽  
Yaolei Wang ◽  
Jianchun Wang ◽  
Li Ren ◽  
Qin Tu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Microfluidic Device

Expression and characterization of an α-glucosidase from Thermoanaerobacter ethanolicus JW200 with potential for industrial application

Biologia ◽  
2009 ◽  
Vol 64 (6) ◽  
Author(s):  
Yue-Hong Wang ◽  
Yu Jiang ◽  
Zuo-Ying Duan ◽  
Wei-Lan Shao ◽  
Hua-Zhong Li
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Optimal Condition ◽  
Industrial Application ◽  
Conversion Rate ◽  
Hydrolytic Activity ◽  
Thermoanaerobacter Ethanolicus ◽  
Transglycosylation Activity

AbstractIn this study, a new α-glucosidase gene from Thermoanaerobacter ethanolicus JW200 was cloned and expressed in Escherichia coli by a novel heat-shock vector pHsh. The recombinant α-glucosidase exhibited its maximum hydrolytic activity at 70°C and pH 5.0∼5.5. With p-nitrophenyl-α-D-glucoside as a substrate and under the optimal condition (70°C, pH 5.5), K m and V max of the enzyme was 1.72 mM and 39 U/mg, respectively. The purified α-glucosidase could hydrolyze oligosaccharides with both α-1,4 and α-1,6 linkages. The enzyme also had strong transglycosylation activity when maltose was used as sugar donor. The transglucosylation products towards maltose are isomaltose, maltotriose, panose, isomaltotriose and tetrasaccharides. The enzyme could convert 400 g/L maltose to oligosaccharides with a conversion rate of 52%, and 83% of the oligosaccharides formed were prebiotic isomaltooligosaccharides (containing isomaltose, panose and isomaltotriose).

scholarly journals Sequence of the lon gene in Escherichia coli. A heat-shock gene which encodes the ATP-dependent protease La.

1988 ◽  
Vol 263 (24) ◽  
pp. 11718-11728 ◽  
Author(s):  
D T Chin ◽  
S A Goff ◽  
T Webster ◽  
T Smith ◽  
A L Goldberg
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Heat Shock Gene ◽  
Shock Gene

Heat shock regulation in the ftsH null mutant of Escherichia coli: dissection of stability and activity control mechanisms of sigma32 in vivo

1998 ◽  
Vol 30 (3) ◽  
pp. 583-593 ◽  
Author(s):  
Takashi Tatsuta ◽  
Toshifumi Tomoyasu ◽  
Bernd Bukau ◽  
Masanari Kitagawa ◽  
Hirotada Mori ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Control Mechanisms ◽  
Null Mutant
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