Exploring the coordination chemistry of isomerizable terpyridine derivatives for successful analyses of cis and trans isomers by travelling wave ion mobility mass spectrometry

Butterfly pea flower have great sensory attraction, but they have not yet been used widely in Vietnam. Extracts of butterfly pea flowers can be used conveniently as a natural blue colorant for food products. In this study, the identification of anthocyanin compounds in butterfly pea flowers was performed by UPLC coupled with a UV and Mass spectrometer instrument. Positive and negative ion electrospray MS/MS chromatograms and spectra of the anthocyanin compounds were determined. By analyzing the chromatograms and spectra for each ion, five anthocyanins were identified in the butterfly pea flower extract; these were delphinidin-3-(6”‐p-coumaroyl)-rutinoside, cyanidin 3-(6”-p-coumaroyl)-rutinoside, delphinidin-3-(p-coumaroyl) glucose in both cis- and trans- isomers, cyanidin-3-(p-coumaroyl-glucoside) and delphinidin-3-pyranoside. Additionally, based on their intensity, it was determined that cyanidin-3-(p-coumaroyl-glucoside) was the most abundant anthocyanin, followed by cyanidin 3-(6”-p-coumaroyl)-rutinoside, delphinidin-3-(p-coumaroyl-glucoside), delphinidin-3-(6”-p-coumaroyl)-rutinoside and delphinidin-3-pyranoside. In this study, cyanidin derivatives were discovered in butterfly pea flower extract, where these compounds had not been detected in previous studies.

Download Full-text

Separation of cis and trans Isomers of Polyproline by FAIMS Mass Spectrometry

Journal of the American Society for Mass Spectrometry ◽

10.1007/s13361-016-1482-1 ◽

2016 ◽

Vol 27 (12) ◽

pp. 2071-2074 ◽

Cited By ~ 4

Author(s):

Andrew J. Creese ◽

Helen J. Cooper

Keyword(s):

Mass Spectrometry ◽

Trans Isomers ◽

Cis And Trans Isomers ◽

Cis And Trans

Download Full-text

Amphitrite: A program for processing travelling wave ion mobility mass spectrometry data

International Journal of Mass Spectrometry ◽

10.1016/j.ijms.2012.09.005 ◽

2013 ◽

Vol 345-347 ◽

pp. 54-62 ◽

Cited By ~ 44

Author(s):

Ganesh N. Sivalingam ◽

Jun Yan ◽

Harpal Sahota ◽

Konstantinos Thalassinos

Keyword(s):

Mass Spectrometry ◽

Ion Mobility ◽

Travelling Wave ◽

Mass Spectrometry Data ◽

Ion Mobility Mass Spectrometry

Download Full-text

Assessment of the gas phase stability of quadruplex DNA using travelling wave ion mobility mass spectrometry

International Journal of Mass Spectrometry ◽

10.1016/j.ijms.2010.10.033 ◽

2011 ◽

Vol 304 (2-3) ◽

pp. 195-203 ◽

Cited By ~ 7

Author(s):

Karina C. Porter ◽

Jennifer L. Beck

Keyword(s):

Mass Spectrometry ◽

Gas Phase ◽

Phase Stability ◽

Ion Mobility ◽

Travelling Wave ◽

Ion Mobility Mass Spectrometry ◽

Quadruplex Dna

Download Full-text

Characterisation of Calmodulin Structural Transitions by Ion Mobility Mass Spectrometry

Australian Journal of Chemistry ◽

10.1071/ch12047 ◽

2012 ◽

Vol 65 (5) ◽

pp. 504 ◽

Cited By ~ 5

Author(s):

Antonio N. Calabrese ◽

Lauren A. Speechley ◽

Tara L. Pukala

Keyword(s):

Mass Spectrometry ◽

Cross Section ◽

Cross Sections ◽

Ion Mobility ◽

Collision Cross Section ◽

Travelling Wave ◽

Ion Mobility Mass Spectrometry ◽

Structural Transitions ◽

Calmodulin Binding ◽

Negative Mode

This study demonstrates the ability of travelling wave ion mobility-mass spectrometry to measure collision cross-sections of ions in the negative mode, using a calibration based approach. Here, negative mode ion mobility-mass spectrometry was utilised to understand structural transitions of calmodulin upon Ca2+ binding and complexation with model peptides melittin and the plasma membrane Ca2+ pump C20W peptide. Coexisting calmodulin conformers were distinguished on the basis of their mass and cross-section, and identified as relatively folded and unfolded populations, with good agreement in collision cross-section to known calmodulin geometries. Titration of calcium tartrate to physiologically relevant Ca2+ levels provided evidence for intermediately metalated species during the transition from apo- to holo-calmodulin, with collision cross-section measurements indicating that higher Ca2+ occupancy is correlated with more compact structures. The binding of two representative peptides which exemplify canonical compact (melittin) and extended (C20W) peptide-calmodulin binding models has also been interrogated by ion mobility mass spectrometry. Peptide binding to calmodulin involves intermediates with metalation states from 1–4 Ca2+, which demonstrate relatively collapsed structures, suggesting neither the existence of holo-calmodulin or a pre-folded calmodulin conformation is a prerequisite for binding target peptides or proteins. The biological importance of the different metal unsaturated calmodulin complexes, if any, is yet to be understood.

Download Full-text