scholarly journals Identification of Anthocyanin Compounds in Butterfly Pea Flowers (Clitoria ternatea L.) by Ultra Performance Liquid Chromatography/Ultraviolet Coupled to Mass Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4539
Author(s):  
Nguyen Minh Thuy ◽  
Vo Minh ◽  
Tran Ben ◽  
My Tuyen Thi Nguyen ◽  
Ho Ha ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ultra Performance Liquid Chromatography ◽  
Negative Ion ◽  
Trans Isomers ◽  
Clitoria Ternatea ◽  
Flower Extract ◽  
Cis And Trans Isomers ◽  
Butterfly Pea ◽  
Cis And Trans

Butterfly pea flower have great sensory attraction, but they have not yet been used widely in Vietnam. Extracts of butterfly pea flowers can be used conveniently as a natural blue colorant for food products. In this study, the identification of anthocyanin compounds in butterfly pea flowers was performed by UPLC coupled with a UV and Mass spectrometer instrument. Positive and negative ion electrospray MS/MS chromatograms and spectra of the anthocyanin compounds were determined. By analyzing the chromatograms and spectra for each ion, five anthocyanins were identified in the butterfly pea flower extract; these were delphinidin-3-(6”‐p-coumaroyl)-rutinoside, cyanidin 3-(6”-p-coumaroyl)-rutinoside, delphinidin-3-(p-coumaroyl) glucose in both cis- and trans- isomers, cyanidin-3-(p-coumaroyl-glucoside) and delphinidin-3-pyranoside. Additionally, based on their intensity, it was determined that cyanidin-3-(p-coumaroyl-glucoside) was the most abundant anthocyanin, followed by cyanidin 3-(6”-p-coumaroyl)-rutinoside, delphinidin-3-(p-coumaroyl-glucoside), delphinidin-3-(6”-p-coumaroyl)-rutinoside and delphinidin-3-pyranoside. In this study, cyanidin derivatives were discovered in butterfly pea flower extract, where these compounds had not been detected in previous studies.

Analysis of d-Phenothrin and Its Optical Isomers in Technical Preparations and Formulations

1985 ◽  
Vol 68 (5) ◽  
pp. 911-916 ◽  
Author(s):  
Tadashi Doi ◽  
Shigeyuki Sakaue ◽  
Masao Horiba
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Optical Isomers ◽  
Isomer Ratio ◽  
Trans Isomers ◽  
Internal Standards ◽  
Cis And Trans Isomers ◽  
Alkaline Conditions ◽  
Cis And Trans

Abstract The content of d-phenothrin in technical preparations was determined by gas chromatography (GC) on a column of 2% PEG-20M or 2% DEGS, using suitable internal standards. The cis- and trans-isomers were separated from each other and determined on a 2% QF-1 column. The 4 optical isomers of rf-phenothrin were hydrolyzed in alkaline conditions to liberate chrysanthemic acid, which was derivatized to diastereoisomeric </-2-octyl esters, and the isomer ratio was determined by GC. The 4 optical isomers were also separated from one another directly on a chiral Sumipax OA-2000 column by liquid chromatography (LC). Also, after the propellant gas in an aerosol formulation was released, and the remaining liquid was analyzed using the same GC conditions as those used for technical preparations

scholarly journals Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Guangqiang Huang ◽  
Jie Liang ◽  
Xiaosi Chen ◽  
Jing Lin ◽  
Jinyu Wei ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Relevant Literature ◽  
Ultra Performance Liquid Chromatography ◽  
Negative Ion ◽  
Volatile Components ◽  
Isolation And Identification ◽  
Fragmentation Patterns ◽  
Nitrogen Containing Compounds

Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules.

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