Assays for human telomerase activity: progress and prospects

2012 ◽  
Vol 41 (13) ◽  
pp. 4643 ◽  
Author(s):  
Xiaoming Zhou ◽  
Da Xing
Keyword(s):  
Telomerase Activity ◽  
Human Telomerase

A multicolor DNA tetrahedron nanoprobe for analyzing human telomerase in living cells

2021 ◽  
Author(s):  
Ruiyuan Zhang ◽  
Ruixue Zhang ◽  
Wei Jiang ◽  
Xiaowen Xu
Keyword(s):  
Real Time ◽  
Length Distribution ◽  
Telomerase Activity ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Dna Tetrahedron ◽  
Human Telomerase

A sequentially lighting-up multicolor DNA tetrahedron nanoprobe is constructed for imaging telomerase activity, real-time monitoring telomerase action and determining product length distribution in living cells.

scholarly journals Gold-Nanoparticle Sensors: Visualizing Human Telomerase Activity with Primer-Modified Au Nanoparticles (Small 2/2012)

Small ◽  
2012 ◽  
Vol 8 (2) ◽  
pp. 166-166
Author(s):  
Jiasi Wang ◽  
Li Wu ◽  
Jinsong Ren ◽  
Xiaogang Qu
Keyword(s):  
Gold Nanoparticle ◽  
Au Nanoparticles ◽  
Telomerase Activity ◽  
Human Telomerase

scholarly journals Telomerase Activity Reconstitutedin Vitrowith Purified Human Telomerase Reverse Transcriptase and Human Telomerase RNA Component

2000 ◽  
Vol 275 (29) ◽  
pp. 22568-22573 ◽  
Author(s):  
Kenkichi Masutomi ◽  
Shuichi Kaneko ◽  
Naoyuki Hayashi ◽  
Tatsuya Yamashita ◽  
Yukihiro Shirota ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Telomerase Activity ◽  
Telomerase Reverse Transcriptase ◽  
Telomerase Rna ◽  
Human Telomerase Reverse Transcriptase ◽  
Human Telomerase

scholarly journals Apoptotic endonuclease EndoG regulates alternative splicing of human telomerase catalytic subunit hTERT

2016 ◽  
Vol 62 (5) ◽  
pp. 544-554 ◽  
Author(s):  
D.D. Zhdanov ◽  
D.A. Vasina ◽  
E.V. Orlova ◽  
V.S. Orlova ◽  
M.V. Pokrovskaya ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Telomerase Activity ◽  
Catalytic Subunit ◽  
Over Expression ◽  
Htert Gene ◽  
Template Strand ◽  
Non Coding Rna ◽  
Dna Strand ◽  
Human Telomerase ◽  
Long Non Coding Rna

Human telomerase catalytic subunit hTERT is subjected to alternative splicing results in loss of its function and leads to decrease of telomerase activity. However, very little is known about the mechanism of hTERT pre-mRNA alternative splicing. Apoptotic endonuclease EndoG is known to participate this process. The aim of this study was to determine the role of EndoG in regulation of hTERT alternative splicing. Increased expression of b-deletion splice variant was determined during EndoG over-expression in CaCo-2 cell line, after EndoG treatment of cell cytoplasm and nuclei and after nuclei incubation with EndoG digested cell RNA. hTERT alternative splicing was induced by 47-mer RNA oligonucleotide in naked nuclei and in cells after transfection. Identified long non-coding RNA, that is the precursor of 47-mer RNA oligonucleotide. Its size is 1754 nucleotides. Based on the results the following mechanism was proposed. hTERT pre-mRNA is transcribed from coding DNA strand while long non-coding RNA is transcribed from template strand of hTERT gene. EndoG digests long non-coding RNA and produces 47-mer RNA oligonucleotide complementary to hTERT pre-mRNA exon 8 and intron 8 junction place. Interaction of 47-mer RNA oligonucleotide and hTERT pre-mRNA causes alternative splicing.

Detection of telomerase activity, human telomerase RNA, and telomerase reverse transcriptase in human hepatocellular carcinoma

2000 ◽  
Vol 118 (4) ◽  
pp. A993
Author(s):  
Masaki Shimojima ◽  
Fumihiko Komine ◽  
Hiroshi Aoki ◽  
Toshihiro Shimizu ◽  
Tamiko Kaneko ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Reverse Transcriptase ◽  
Telomerase Activity ◽  
Human Hepatocellular Carcinoma ◽  
Telomerase Reverse Transcriptase ◽  
Telomerase Rna ◽  
Human Telomerase

Curcumin inhibits telomerase activity through human telomerase reverse transcritpase in MCF-7 breast cancer cell line

2002 ◽  
Vol 184 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Cheppail Ramachandran ◽  
Hugo B Fonseca ◽  
Perseus Jhabvala ◽  
Enrique A Escalon ◽  
Steven J Melnick
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Telomerase Activity ◽  
Human Telomerase ◽  
Mcf 7

Quantitative assays for measuring human telomerase activity and DNA binding properties

Methods ◽  
2017 ◽  
Vol 114 ◽  
pp. 85-95 ◽  
Author(s):  
Christopher G. Tomlinson ◽  
Natsuki Sasaki ◽  
Julie Jurczyluk ◽  
Tracy M. Bryan ◽  
Scott B. Cohen
Keyword(s):  
Dna Binding ◽  
Telomerase Activity ◽  
Binding Properties ◽  
Human Telomerase ◽  
Dna Binding Properties
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