In this study, an ultrasensitive surface-enhanced Raman scattering (SERS) detection of alkaline phosphatase (ALP) has been developed, in which nile blue A (NBA) was chosen to replace nitro blue tetrazolium chloride (NBT) in a reactive system of 5-bromo-4-chloro-3-indolyl phosphate (BCIP), NBT, and ALP.
In this work we describe the development of an optofluidic device for surface enhanced Raman scattering (SERS) based detection of biological pathogens. The chip exploits the use of electro-active microwells which serve to both physically concentrate the Raman enhancers and to reduce the total analysis time through a unique electrokinetically driven on-chip mixing effect. To quantify the concentration performance of the device we use 44 nm polystyrene particles at low electric field strength (between 1.00–2.00 V) and demonstrate close to 90% concentration saturation within 2.5 s. We demonstrate the mixing capability through the enhanced detection of dengue virus serotype 2 (DENV-2). With DENV-2, we successfully detected the SERS signals with a limit of detection of 30 pM.
A dielectrophoretic chip with a roughened metal surface for on-chip surface-enhanced Raman scattering analysis of bacteria